1996 Fiscal Year Annual Research Report

化学感覚細胞における新しい細胞内情報変換機構とそれに関与する受容体のクローニング

Research Project

Project/Area Number	07457533
Research Institution	HOKKAIDO UNIVERSITY
Principal Investigator	栗原賢三北海道大学, 薬学部, 教授 (00016114)
Co-Investigator(Kenkyū-buntansha)	庄司隆行北海道大学, 薬学部, 教務職員 (00241349) 柏柳誠北海道大学, 薬学部, 助手 (20169436) 松岡一郎北海道大学, 薬学部, 助手 (40157269)
Keywords	ファスファチジン酸 / リポ蛋白質 / G蛋白質共役型受容体 / フェロモン / 尿 / 鋤鼻器 / IP_3 / ラット
Research Abstract	先に我々が開発したフォスファチジン酸とβ-ラクトグロブリンからなるリポ蛋白質は,各種の苦味を選択的に抑制する.このリポ蛋白質をカエルの舌に作用させ,苦味を与えたときの味神経のインパルスを測定した.その結果,調べたほとんどの薬物の苦味は抑制されたが,MgCl_2, SsCl,テトラエチルアンモニウムのような塩の苦味は抑制されなかった.明らかに,これらの薬物の受容体は他の薬物の受容体とは異なることがわかった.ついで,カエルの嗅細胞を用いて,単一の嗅細胞がどのくらいの匂いに応答するかを調べた.その結果,単一の嗅細胞は,多数の匂いに応答することがわかった.G蛋白質共役型の受容体は,一個の細胞に一種類した含まれていないので,今回得られた我々の結果は,匂いの受容はG蛋白質共役型受容体だけを介するという機構では説明できない.別の受容体が存在することが示唆された.フェロモン受容体である鋤鼻器感覚組織のスライスを作製し,セカンドメッセンジャー候補の活性を調べた.ラット鋤鼻器細胞を用いたときには,cAMPを注入しても応答は発現しなかった.これにたいして,IP3にはよく応答した.尿中にはフェロモン様物質が含まれているので,部分精製したオスラットの尿標品をメスの鋤鼻器感覚細胞に与えた.この結果,オスの細胞はインパルスを発生した.この応答は,IP3産生の阻害剤で抑制されたので,尿標品のフェロモン情報は,IP3経路を介して発現することが明らかになった.

Research Products
(22 results)

All Other

All Publications (22 results)

[Publications] Y.Katsuragi: "Lipoprotein that selectively inhibits taste nerve responses to bitter substances" Brain Res.713. 240-245 (1996)
[Publications] Y.Katsuragi: "Characteristics of phosphatidic acid-containing lipoproteins which selectively inhibit bitter taste ; high affinity to frog tongue surface and hydrophobic model membranes" Biochem. Biophys. Acta. 1289. 322-328 (1996)
[Publications] H. Nagao: "Effects of phorbol ester on expression of CNTF-mRNA in cultured astrocytes from rat olfactory Bulb" Brain Res. 719. 23-28 (1996)
[Publications] M. Kashiwayanagi: "Odor discrimination of "cAMP-" and "IP_3-dependent" odorants at turtle olfactory bulb" Eur. J. Physiol. 431. 786-790 (1996)
[Publications] M. Kashiwayanagi: "Ca^<2+>-and Cl^--dependence of the turtle olfactory response to forskokin" Comp. Biochem. Physiol. 115A. 43-52 (1996)
[Publications] M. Taniguchi: "Intracellular dialysis of cyclic nucleotide induces inward currents in the turtle vomeronasal receptor neurons" J. Neuersci. 16. 1239-1246 (1996)
[Publications] T. Shoji: "Olfactory responses if a euryhaline fish, The rainbow trout : adaptation of olfactory receptors to sea water and salt dependence of their responses to amino acids" J. Exp. Biol.199. 303-310 (1996)
[Publications] K. Okamoto: "Adenylyl cyclase activity in turtle vomeronasal and olfactory epithelium" Biochem. Biophys. Res. Commun.220. 98-101 (1996)
[Publications] M. Kashiwayanagi: "Existence of multiple receptors in single reurons : responses of single bullfrog olfactory neurons to many cAMP-dependent and independent odorants" Brain. Res.738. 222-228 (1996)
[Publications] M. Kashiwayanagi: "Enhancement of turtle olfactory reponses to forskolin and odorants by increasing mucosal Na^+ concentration" Prim. Sensory Neuron.1. 285-296 (1996)
[Publications] M. Kashiwayanagi: "Specific receptor-and non-receptor-mediated odor responses and their multiple transduction pathways" Prim. Sensory Neuron.1. 311-325 (1996)
[Publications] M. Taniguchi: "Difference in behavior between responses to forskolin and general odorants in turtle vomeronasal organ" Chemical Senses.21. 763-771 (1996)
[Publications] M. Kashiwayanagi: "Dialysis of inositol 1, 4, 5-trisphosphate induces inward currents and Ca^<2+> uptake in frog olfactory receptor cells" Biochem. Biophys. Res. Commun.225. 666-671 (1996)
[Publications] 柏柳誠: "嗅覚・味覚系におけるGTP結合蛋白質共役型受容体" 実験医学. 14. 68-70 (1996)
[Publications] 柏柳誠: "生体膜表面における化学識別-匂い受容膜を例として-" 化学工業. 47. 921-926 (1996)
[Publications] K. Inamura: "Inositol 1, 4, 5-trisphosphate induces responses in receptor neurons in rat vomeronasal sensory slices" Chem. Senser. 22. 93-104 (1997)
[Publications] M. Kashiwayanagi: "Long period recording of olfactory and vomeronasal stimulant-induced waves from the turtle main olfactory bulb and accessory olfactory bulb" Brain Res. Protocols. (印刷中).
[Publications] Y. Katsuragi: "Specific inhibitor for bitter taste : inhibition of frog taste nerve responses and human taste sensation to bitter stimuli" Brain Res.(印刷中).
[Publications] Y. Katsuragi: "Basic studies for the practical use of bitterness inhibitors ; Selective inhibition of bitterness by phospholipids" Pharm Res.(印刷中).
[Publications] K. Inamura: "Odor discrimination of "cAMP-" and "IP_3-dependent" odorants at high temperature and at high ion concentration" J. Physiol. Paris. (印刷中).
[Publications] 柏柳誠: "脳・神経" 羊士社(印刷中),
[Publications] 柏柳誠: "感覚情報-生物の巧みなシグナルのやり取り" 吉岡書店(印刷中),

1996 Fiscal Year Annual Research Report

化学感覚細胞における新しい細胞内情報変換機構とそれに関与する受容体のクローニング

Principal Investigator

栗原 賢三 北海道大学, 薬学部, 教授 (00016114)

Research Products

[Publications] Y.Katsuragi: "Lipoprotein that selectively inhibits taste nerve responses to bitter substances" Brain Res.713. 240-245 (1996)

[Publications] Y.Katsuragi: "Characteristics of phosphatidic acid-containing lipoproteins which selectively inhibit bitter taste ; high affinity to frog tongue surface and hydrophobic model membranes" Biochem. Biophys. Acta. 1289. 322-328 (1996)

[Publications] H. Nagao: "Effects of phorbol ester on expression of CNTF-mRNA in cultured astrocytes from rat olfactory Bulb" Brain Res. 719. 23-28 (1996)

[Publications] M. Kashiwayanagi: "Odor discrimination of "cAMP-" and "IP_3-dependent" odorants at turtle olfactory bulb" Eur. J. Physiol. 431. 786-790 (1996)

[Publications] M. Kashiwayanagi: "Ca^<2+>-and Cl^--dependence of the turtle olfactory response to forskokin" Comp. Biochem. Physiol. 115A. 43-52 (1996)

[Publications] M. Taniguchi: "Intracellular dialysis of cyclic nucleotide induces inward currents in the turtle vomeronasal receptor neurons" J. Neuersci. 16. 1239-1246 (1996)

[Publications] T. Shoji: "Olfactory responses if a euryhaline fish, The rainbow trout : adaptation of olfactory receptors to sea water and salt dependence of their responses to amino acids" J. Exp. Biol.199. 303-310 (1996)

[Publications] K. Okamoto: "Adenylyl cyclase activity in turtle vomeronasal and olfactory epithelium" Biochem. Biophys. Res. Commun.220. 98-101 (1996)

[Publications] M. Kashiwayanagi: "Existence of multiple receptors in single reurons : responses of single bullfrog olfactory neurons to many cAMP-dependent and independent odorants" Brain. Res.738. 222-228 (1996)

[Publications] M. Kashiwayanagi: "Enhancement of turtle olfactory reponses to forskolin and odorants by increasing mucosal Na^+ concentration" Prim. Sensory Neuron.1. 285-296 (1996)

[Publications] M. Kashiwayanagi: "Specific receptor-and non-receptor-mediated odor responses and their multiple transduction pathways" Prim. Sensory Neuron.1. 311-325 (1996)

[Publications] M. Taniguchi: "Difference in behavior between responses to forskolin and general odorants in turtle vomeronasal organ" Chemical Senses.21. 763-771 (1996)

[Publications] M. Kashiwayanagi: "Dialysis of inositol 1, 4, 5-trisphosphate induces inward currents and Ca^<2+> uptake in frog olfactory receptor cells" Biochem. Biophys. Res. Commun.225. 666-671 (1996)

[Publications] 柏柳誠: "嗅覚・味覚系におけるGTP結合蛋白質共役型受容体" 実験医学. 14. 68-70 (1996)

[Publications] 柏柳誠: "生体膜表面における化学識別-匂い受容膜を例として-" 化学工業. 47. 921-926 (1996)

[Publications] K. Inamura: "Inositol 1, 4, 5-trisphosphate induces responses in receptor neurons in rat vomeronasal sensory slices" Chem. Senser. 22. 93-104 (1997)

[Publications] M. Kashiwayanagi: "Long period recording of olfactory and vomeronasal stimulant-induced waves from the turtle main olfactory bulb and accessory olfactory bulb" Brain Res. Protocols. (印刷中).

[Publications] Y. Katsuragi: "Specific inhibitor for bitter taste : inhibition of frog taste nerve responses and human taste sensation to bitter stimuli" Brain Res.(印刷中).

[Publications] Y. Katsuragi: "Basic studies for the practical use of bitterness inhibitors ; Selective inhibition of bitterness by phospholipids" Pharm Res.(印刷中).

[Publications] K. Inamura: "Odor discrimination of "cAMP-" and "IP_3-dependent" odorants at high temperature and at high ion concentration" J. Physiol. Paris. (印刷中).

[Publications] 柏柳誠: "脳・神経" 羊士社(印刷中),

[Publications] 柏柳誠: "感覚情報-生物の巧みなシグナルのやり取り" 吉岡書店(印刷中),

栗原賢三北海道大学, 薬学部, 教授 (00016114)