1996 Fiscal Year Final Research Report Summary
Reinforcement of ability of carbon dioxide fixation of a themophilic cyanobacter ium and its application toward conversion of carbon dioxide into organic resource
| Project/Area Number |
07555254
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 試験 |
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| Research Field |
生物・生体工学
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| Research Institution | The University of Tokyo |
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Principal Investigator |
IGARASHI Yasuo The University of Tokyo, Graduate School of Agricultural and LIfe Sciences, Professor, 大学院・農学生命科学研究科, 教授 (90114363)
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| Co-Investigator(Kenkyū-buntansha) |
SAIKI Hiroshi Central Research Institute of Electric Power Industry, Bio-technology Department, バイオラクノロジー特別研究所, 室長
ISGII Masaharu The University of Tokyo, Graduate School of Agricultural and Life Sciences, Prof, 大学院・農学生命科学研究科, 助手 (30193262)
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| Project Period (FY) |
1995 – 1996
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| Keywords | cyanobacterium / carbon dioxide fixation / phycocyanin / carbon dioxide |
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| Research Abstract |
Condition for the dense culture of a thermophilic cyanobacterium Chroococcidiopsis sp. strain TS-821 was determined by using a 1-liter photobioreactor. In the next step, in order to know how CO_2 is converted within the cells, content of protein, suger, and lipid within the lyophilized cells were determined. The results were as follows ; protein 49%, sugar 37%, and lipid 12%.
The supernatant solution after centrifugation of the lyophilized cells gave a dense blue color. The substance, which was assumed to be phycocyanin, was purified to electric homogeneity by using Superose-6 and Mono-Q column chromatography. The protein was composed of two subunits ; amino acid sequences of the subunits confirmed that the protein is phycocyanin.
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