1997 Fiscal Year Final Research Report Summary
Development of T-cell vaccine for hepatitis C virus
| Project/Area Number |
07557047
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Gastroenterology
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| Research Institution | Jichi Medical School |
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Principal Investigator |
IMAWARI Michio Jichi Medical School, Department of Medicine, Professor, 医学部, 教授 (70134228)
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| Co-Investigator(Kenkyū-buntansha) |
KANEKO Takashi Jichi Medical School, Department of Medicine, Associate, 医学部, 助手 (10254913)
MORIYAMA Takashi Jichi Medical School, Department of Medicine, Instructor, 医学部, 講師 (10240706)
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| Project Period (FY) |
1995 – 1997
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| Keywords | hepatitis C virus / hepatitis C / cytotoxic T cell / DNA vaccine |
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| Research Abstract |
We tried to develop T-cell vaccine to prevent and treat hepatitis C virus (HCV) infection. We established cytotoxic T cell clones from peripheral blood lymphocytes of two HLA B44-positive patients with chronic HCV infection that recognize HCV core antigen 88-96 as a minimal and optimal epitope. We demonstrated that positions 4 and 6 of the 9 amino acid epitope were important for the binding of the epitope and T-cell antigen receptors of the CTL clones. When amino acids at positions 4 and 7 were substituted by other amino acids, a wild type epitope for type 1b and 2a HCV (NEGLGWAGW) and a variant type for 2b HCV (NEGCGWMGW) were recognized most efficitntly by CTL slones. HCV DNA that express HCV core antigen in the tranfected mammalian cells induced HCV-specific CTL when the DNA was injected directly into the spleen of mice. Intramuscular injection of the DNA did not induce CTL in either mice or chimpanzees.
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