1996 Fiscal Year Final Research Report Summary
Development of a new bioartificial liver module
| Project/Area Number |
07557357
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 試験 |
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| Research Field |
General surgery
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| Research Institution | University of Tokyo |
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Principal Investigator |
MUTO Tetsuichiro University of Tokyo, Fac of Med, Professor, 医学部・附属病院, 教授 (20110695)
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| Co-Investigator(Kenkyū-buntansha) |
SAKAI Yasuyuki University of Tokyo, Ins of Industrial Science, Assistar, 生産技術研究所, 助手 (00235128)
SUZUKI Motoyuki University of Tokyo, Ins of Industrial Science, Professo, 生産技術研究所, 教授 (10011040)
NARUSE Katsutoshi University of Tokyo, Fac of Med, Assistant staff, 医学部・附属病院, 助手
NAGASHIMA Ikuo University of Tokyo, Fac of Med, Lecturer, 医学部・附属病院, 講師 (90202423)
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| Project Period (FY) |
1995 – 1996
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| Keywords | Hybrid bioartificial liver / Bioreactor / Polyesten nan-woven fabric / 肝細胞スフェロイド / ブタ肝細胞 / 同種潅流治療 |
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| Research Abstract |
We devised a bioreactor with high hepatic functional capacity by employing large unmbers of viable hepatocytes and allowing direct contact between the hepatocytes and blood. The newly-developed bioreactor was filled with porcine hepatocytes immobilized on non-woven polyester fabric (NWF) and was given the name NWF bioreactor. Our most recently developed NWF bioreactor is a 200-mL radial-flow-type polycarbonate column, which can accommodate a maximum of 1.3*10^<10> hepatocytes per bioreactor unit. We investigated in vitro efficiency of this bioreactor and obtained promicing results. We utilized a porcine operative liver failure model, and investigated the efficacy of the NWF bioreactor in homologous perfusion conducted to treat pigs with liver failure. Our system of homologous perfusion using a NWF bioreactor has shown promising results in the treatment of pigs with severe liver failure. Our ultimate goal is to treat patients by extracorpoleal homologous perfusion using a NWF bioreactor filled with proliferated human hepatocytes. Our hope is that a hybrid bioartificial liver system incorporating the NWF bioreactor can be used clinically in the future.
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