1995 Fiscal Year Annual Research Report

生物学的作用解析用高磁場発生装置の開発と個体〜遺伝子レベルでの磁場効果解明

Research Project

Project/Area Number	07558203
Research Category	Grant-in-Aid for Developmental Scientific Research (B)
Research Institution	Kanazawa University
Principal Investigator	山本博金沢大学, 医学部, 教授 (00115198)
Co-Investigator(Kenkyū-buntansha)	細野隆次金沢大学, 医学部, 講師 (40019617) 谷本能文広島大学, 理学部, 助教授 (10110743) 山田外史金沢大学, 工学部, 教授 (80019786)
Keywords	変動高磁場 / 生体作用 / 線虫 / 成長 / 生殖 / 遺伝子 / ミドリムシ / 走化性
Research Abstract	1.冷却灌流装置とビデオ監視記録装置付き交流高磁場発生装置の作製に成功した。1200V、60Hzで最大ピーク値5テスラの交流高磁場が得られ、90l/minの冷却水灌流により±0.5℃以内の精度で磁場空間を生理的温度に保つことができた。また、次年度計画の遺伝子複製・転写実験ではラジオアイソトープ標識ヌクレオチドを用いることが必須となるため、放射線管理区域内で使用可能な冷却装置付き小型高磁場発生装置も試作した。この装置は、励磁電流200V、192Aのとき実験空間70×100×6mm^3において周波数60Hz、波高値1テスラ、均一度2%以内の磁界を発生しうる。 2.種々の発生段階に同調培養した線虫にピーク値1.7テスラの高磁場を印加した。その結果、孵化後の体長増加と産卵開始時期が非磁場印加群に比し遅延する傾向が認められたが、いずれの形質も磁場解除から一定時間後には正常に復した。器官形成には異常は認められなかった。また、hermaphroditeの産卵数は磁気線量に依存して軽度減少することが見い出された。 3.遺伝子複製・転写反応のための鋳型DNA・プライマーDNAを化学合成しHPLCで精製した。 4.なお、本研究遂行の過程で、ミドリムシが強い勾配磁場中で高磁場方向に走化性を示すことも発見された。

Research Products
(26 results)

All Other

All Publications (26 results)

[Publications] Kazuo Bessho: "Biological responses in Caenorhabditis elegans to high magnetic fields" EXPERIENTIA. 51. 284-288 (1995)
[Publications] Sotoshi YAMADA: ""Effects of High AC Magnetic Field Stimulus to the Nematode"" ADVANCED COMPUTATIONAL AND DESIGN TECHNIQUES IN APPLIED ELECTROMAGNETIC SYSTEMS. 181-184 (1995)
[Publications] Hong Cao: "Effect of High Magnetic Field on the Decay Rate of Chain-Linked Illntramolecular Exciplex Fluorescence" Chemical Physics Letters. 246. 171-175 (1995)
[Publications] Akio Katsuki: "The Effects of High Magnetic Field on the Deposition of Silver" Chemistry Letters. 219-220 (1996)
[Publications] Hisamitsu Ogawa: "Expression, Purification, and Characterization of Recombinant C. elegans UNC-18." Neurochemistry International. (in press). (1996)
[Publications] Toshihiro Sassa: "The Synaptic Protein UNC-18 is Phosphorylated by Protein kinase C." Neurochemistry International. (in press). (1996)
[Publications] Hiroshi Yamamoto: ""Vascular VEGF"in Hypoxia-Induced Proliferation of Vascular Cells." Microcirculation Annual. 11. 11-12 (1996)
[Publications] Motohiro Nomura: "Possible Participation of Autocrine and Paracrine Vascular Endothelial Growth Factors in Hypoxia-Induced Proliferation of Endothelial Cells and Pericytes." J. Biol. Chem.270. 28316-28324 (1995)
[Publications] Sho-ichi Yamagishi: "Receptor-Mediated Toxicity to Pericytes of Advanced Glycosylation End Products:" Biochem. Biophys. Res. Commun.213. 681-687 (1995)
[Publications] Hitomi Takanaga: "cDNA cloning and functional characterization of rat intestinal monocarboxylate transporter." Biochem. Biophys. Res. Commun.217. 370-377 (1995)
[Publications] Emi Nakashima: "Reversal of Drug Sensitivity in MDR Subline of P388 Leukemia by Gene-Targeted Antisense Oligonucleotide." J. Pharmaceutical Sciences. 84. 1205-1209 (1995)
[Publications] Takeda Ryoyu: "Aldosterone biosynthesis and action in vascular cells." Steroids. 60. 120-124 (1995)
[Publications] Motohiro Nomura: "Expression and function of VEGF gene in hypoxia-induced proliferation of vascular cells" Antisense Research and Development. 5. 91 (1995)
[Publications] Sho-ichi Yamagishi: "A role of AGE receptor in the development of diabetic microangiopathy" Antisense Research and Development. 5. 93 (1995)
[Publications] Masahide Kaji: "Antisense oligonucleotides against c-met mRNA inhibit the growth of human gastric cancer cells." Antisense Research and Development. 5. 99-100 (1995)
[Publications] Kayo Uchiyama: "Topical fluconazole: high penetration without corneal toxicity." lens and eye toxicity research. 181-190 (1995)
[Publications] Sho-ichi Yamagishi: "Advanced glycosylation end products stimulate the growth but inhibit the prostacy clin-producing ability of endothelial cells through interactions with their receptors" FEBS Lett.(in press). (1996)
[Publications] 山田外史: "「低周波交流磁界による生体内化学反応への影響」" 電気学会論文誌. 116-C. 187-192 (1996)
[Publications] 谷本能文: "界面活性剤ミセル水溶液中の光化学反応と磁場効果" 表面. 33. 282-290 (1995)
[Publications] 谷本能文: "強磁場中の有機化学" 有機合成化学協会誌. 53. 413-422 (1995)
[Publications] 山本博: "試験管内「血液脳関門」モデル" 学術月報(Japanese Scientific Monthly). 48. 1233 (1995)
[Publications] 山本博: "アンチセンスDNA" 生化学. 68. 155 (1996)
[Publications] 野村素弘: "血管の再構築とその病態" 最新医学社, 138 (1995)
[Publications] 山本博: "持田記念財団研究成果報告書" (財)持田記念医学薬学振興財団, 287 (1995)
[Publications] 山本博: "周皮細胞、「血管新生のメカニズムと疾患」" 医薬ジャーナル社(印刷中), (1996)
[Publications] 山本博: "KEY WORD 1996-'97『炎症・免疫系』" 先端医学社, 191 (1996)

1995 Fiscal Year Annual Research Report

生物学的作用解析用高磁場発生装置の開発と個体〜遺伝子レベルでの磁場効果解明

Principal Investigator

山本 博 金沢大学, 医学部, 教授 (00115198)

Research Products

[Publications] Kazuo Bessho: "Biological responses in Caenorhabditis elegans to high magnetic fields" EXPERIENTIA. 51. 284-288 (1995)

[Publications] Sotoshi YAMADA: ""Effects of High AC Magnetic Field Stimulus to the Nematode"" ADVANCED COMPUTATIONAL AND DESIGN TECHNIQUES IN APPLIED ELECTROMAGNETIC SYSTEMS. 181-184 (1995)

[Publications] Hong Cao: "Effect of High Magnetic Field on the Decay Rate of Chain-Linked Illntramolecular Exciplex Fluorescence" Chemical Physics Letters. 246. 171-175 (1995)

[Publications] Akio Katsuki: "The Effects of High Magnetic Field on the Deposition of Silver" Chemistry Letters. 219-220 (1996)

[Publications] Hisamitsu Ogawa: "Expression, Purification, and Characterization of Recombinant C. elegans UNC-18." Neurochemistry International. (in press). (1996)

[Publications] Toshihiro Sassa: "The Synaptic Protein UNC-18 is Phosphorylated by Protein kinase C." Neurochemistry International. (in press). (1996)

[Publications] Hiroshi Yamamoto: ""Vascular VEGF"in Hypoxia-Induced Proliferation of Vascular Cells." Microcirculation Annual. 11. 11-12 (1996)

[Publications] Motohiro Nomura: "Possible Participation of Autocrine and Paracrine Vascular Endothelial Growth Factors in Hypoxia-Induced Proliferation of Endothelial Cells and Pericytes." J. Biol. Chem.270. 28316-28324 (1995)

[Publications] Sho-ichi Yamagishi: "Receptor-Mediated Toxicity to Pericytes of Advanced Glycosylation End Products:" Biochem. Biophys. Res. Commun.213. 681-687 (1995)

[Publications] Hitomi Takanaga: "cDNA cloning and functional characterization of rat intestinal monocarboxylate transporter." Biochem. Biophys. Res. Commun.217. 370-377 (1995)

[Publications] Emi Nakashima: "Reversal of Drug Sensitivity in MDR Subline of P388 Leukemia by Gene-Targeted Antisense Oligonucleotide." J. Pharmaceutical Sciences. 84. 1205-1209 (1995)

[Publications] Takeda Ryoyu: "Aldosterone biosynthesis and action in vascular cells." Steroids. 60. 120-124 (1995)

[Publications] Motohiro Nomura: "Expression and function of VEGF gene in hypoxia-induced proliferation of vascular cells" Antisense Research and Development. 5. 91 (1995)

[Publications] Sho-ichi Yamagishi: "A role of AGE receptor in the development of diabetic microangiopathy" Antisense Research and Development. 5. 93 (1995)

[Publications] Masahide Kaji: "Antisense oligonucleotides against c-met mRNA inhibit the growth of human gastric cancer cells." Antisense Research and Development. 5. 99-100 (1995)

[Publications] Kayo Uchiyama: "Topical fluconazole: high penetration without corneal toxicity." lens and eye toxicity research. 181-190 (1995)

[Publications] Sho-ichi Yamagishi: "Advanced glycosylation end products stimulate the growth but inhibit the prostacy clin-producing ability of endothelial cells through interactions with their receptors" FEBS Lett.(in press). (1996)

[Publications] 山田外史: "「低周波交流磁界による生体内化学反応への影響」" 電気学会論文誌. 116-C. 187-192 (1996)

[Publications] 谷本能文: "界面活性剤ミセル水溶液中の光化学反応と磁場効果" 表面. 33. 282-290 (1995)

[Publications] 谷本能文: "強磁場中の有機化学" 有機合成化学協会誌. 53. 413-422 (1995)

[Publications] 山本博: "試験管内「血液脳関門」モデル" 学術月報(Japanese Scientific Monthly). 48. 1233 (1995)

[Publications] 山本博: "アンチセンスDNA" 生化学. 68. 155 (1996)

[Publications] 野村素弘: "血管の再構築とその病態" 最新医学社, 138 (1995)

[Publications] 山本博: "持田記念財団 研究成果報告書" (財)持田記念医学薬学振興財団, 287 (1995)

[Publications] 山本博: "周皮細胞、「血管新生のメカニズムと疾患」" 医薬ジャーナル社(印刷中), (1996)

[Publications] 山本博: "KEY WORD 1996-'97『炎症・免疫系』" 先端医学社, 191 (1996)

山本博金沢大学, 医学部, 教授 (00115198)

[Publications] 山本博: "持田記念財団研究成果報告書" (財)持田記念医学薬学振興財団, 287 (1995)