1996 Fiscal Year Final Research Report Summary
Establishment of SCID mouse metastatic model of human malignant lymphoma and analysis of lectin binding properties on metastatic capacity
| Project/Area Number |
07670209
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Fukushima Medical College |
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Principal Investigator |
ABE Masafumi Fukushima Medical College, Professor First Department of Pathology, 医学部, 教授 (00045783)
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| Co-Investigator(Kenkyū-buntansha) |
TASAKI Kazuhiro Fukushima Medical College, Instructor First Department of Pathology, 医学部, 助手 (70244382)
TOMINAGA Kunihiko Fukushima Medical College, Assistant Lecture First Department of Pathology, 医学部, 講師 (20145626)
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| Project Period (FY) |
1995 – 1996
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| Keywords | human B-cell lymphoma / SCID mouse / metastasis / lectin / carbohydrate / prognosis |
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| Research Abstract |
1. Establishment of a metastatic SCID mouse model of human B-cell lymphoma lines :
The human B-cell lymphoma lines (HBL-1, HBL-2, HBL-4, HBL-5, HBL-6, HBL-7, HBL-8, Daudi and Raji) were heterotransplanted subcutaneously into SCID mice. HBL-2, HBL-7 and HBL-8 cell lines showed a high spontaneous metastasis, whereas the other cell lines showed no or low-spontaneous metastasis. These high metastatic cell lines may be a useful tool to elucidate the metastatic mechanisms of human B-cell lymphoma.
2. The relation between the binding sites for Glycine Max (SBA) lectin masked by sialylation and metastatic capacity in a SCID mouse model :
A difference in cell surface carbohydrates between high-and no-or low-spontaneous metastatic human B-cell lymphoma lines were analyzed using lectin staining. The results showed that the masking of SBA lectin binding sites by sialic acid might be closely associated with metastatic capacity of human B-cell lymphoma lines in a SCID mouse model
3. The relation between the lectin binding properties (VVA, ABA and PHA-L) and survival in 46 cases with human diffuse large B-cell lymphoma :
The lectin binding properties on 46 cases with diffuse large B-cell lymphoma was analyzed in relation to survival. The lectin binding properties (VVA,ABA and PHA-L) were correlated with survival of patients with diffuse large B-cell lymphoma : The patients with lack or alterations of lectin binding sites of carbohydrates showed a worse prognosis in comparison to those without it.
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