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1996 Fiscal Year Final Research Report Summary

DNA ploidy and S-phase fraction of neoplastic and non-neoplastic lesions of the human gallbladder.

Research Project

Project/Area Number 07670214
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Human pathology
Research InstitutionSchool of Medicine Iwate medical University

Principal Investigator

NAKAMURA Shin-chi  Iwate Medical University, professor, 医学部, 教授 (20107816)

Project Period (FY) 1995 – 1996
Keywordscrypt isolation technique / gallbladder / polyp / gallbladder cancer / flow cytometry / DNA content / proliferative activity / S-phase fraction
Research Abstract

Studies on the cell kinetics of the human gallbladder are difficult because of epithelial degeneration by bile. Using the epithelial isolation technique, however, we were able to determine the degree of degeneration and to examine the cell kinetics of gallbladder lesions in freshly resected surgical specimens. Normal and neoplastic epithelia were isolated nonenzymatically from freshly resected gallbladder. The nuclear DNA content and S-phase fraction were estimated in 110 patients with gallbladder lesions by flow cytometry (FCM). Normal tissues and all lesions except carcinomas were diploid. The S-phase fraction of gallstone cases was significantly higher (1.47(]SY+-[)0.70% ; mean(]SY+-[)SD) than normal (0.79(]SY+-[)0.39%) (p<0.0006). All gallbladder carcinomas were multiploid, and their S-phase fraction was 11.63(]SY+-[)3.65%. Cell renewal of normal gallbladder is low. In the gallstone cases, the S-phase fraction was increased, possibly correlated with carcinogenesis.

  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] S.Nakamura: "Flow cytometric analysis of DNA synthetic phase fraction of the normal appearing colonic mucosa in patients with colorectal neoplasms." Gut. 37. 398-401 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y,Kitayama: "Expression of p53 and flow cytometric DNA analysis of isolated neoplastic glands of the stomach : an application of the gland isolation method." Virchows Arch. 426. 557-562 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.kitayama: "Cytophotometric and flow cytometric DNA content of isolated glands in gastric neoplasia." Gut. 36. 516-521 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中村眞一: "ヒト大腸腺管の分離とその応用" 医学のあゆみ. 177. 486-487 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] W.Habano: "A novel method for gene analysis of colorectal carcinomas using a crypt isolation technique." Lab Invest. 74. 933-940 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Yoshida: "DNA ploidy and S-phase fraction of neoplastic and non-neoplastic lesions of the human gallbladder." J Surg Oncol. 63. 9-16 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S Nakamura, J Goto, Y Kitayama, JP Sheffield, IC Talbot: "Flow cytometric analysis of DNA synthetic phase fraction of the normal appearing colonic mucosa in patients with colorectal neoplasms." Gut. 37. 398-401 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y Kitayama, H Sugimura, M tanaka, S Nakamura, I Kino: "Expression of p53 and flow cytometric DNA analysis of isolated neoplastic glands of the stomach : an application of the gland isolation method." Virchows Arch. 426. 557-562 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y Kitayama, S Nakamura, H Sugimura, I Kino: "Cytophotometric and flow cytometric DNA content of isolated glands in gastric neoplasia." Gut. 36. 516-521 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] W Habano, T Sugai, S Nakamura, T Yoshida: "A novel method for gene analysis of colorectal carcinomas using a crypt isolation technique." Lab Invest. 74. 933-940 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T Yoshida, S Nakamura, T Sugai: "DNA ploidy and S-phase fraction of neoplastic and non-neoplastic lesions of the human gallbladder." J Surg Oncol. 63. 9-16 (1996)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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