1997 Fiscal Year Final Research Report Summary
Ploidy of a black yeast Exophiala dermatitidis
| Project/Area Number |
07670300
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Chiba University |
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Principal Investigator |
TAKEO Kanji Research Center for Pathogenic Fungi and Microbial Toxicoses Chiba University Professor, 真菌医学研究センター, 教授 (10027097)
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Reiko Research Center for Pathogenic Fungi and Microbial Toxicoses Research Associate, 真菌医学研究センター, 助手 (60143319)
NISHIMURA Kazuko Research Center for Pathogenic Fungi and Microbial Toxicoses Chiba University Pr, 真菌医学研究センター, 教授 (00114314)
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| Project Period (FY) |
1995 – 1997
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| Keywords | Exophiala dermatitidis / ploidy / haploid / black yeasts / pathogenic fungi / diploid / DNA stain / nucleus |
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| Research Abstract |
A pathogenic black yeast Exophiala dematitidis was pleomorphic, and took yeast, pseudomycelium, mycelium, multicellular bodies, and two types of conidia. After fixation with 70% ethanol at 4ﾟC,cells (5-10mg) were suspended in test tube in 1 m1 of NS buffer supplemented with 5 mug/ml propidium iodide and lmg/ml RNase. The intensity of the fluorescence was measured with an Olympus BHS-RFC epifluorescence microscope equipped with a light intensity detector Olympus OSP-1.
Yeast cells were easiest to stain nuclear DNA quantitatively, followed by yeast-like pseudomycelia. These cells were obtained in most strains examined after incubation with shaking for one day in glucose, yeast extract and polypeptone medium (1% each) at 25ﾟC or 30ﾟC.
Most strains (53) of E.dermatitidis examined revealed similar sizes of the cells and of nuclei, and the amounts of DNA per cell were usual (unit amount). They were concluded as haploid. Three strains, however, exhibited clearly larger size of cells and nuclei and double amount of DNA per cell compared with usual strains. Thus, they were concluded as diploid.
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