1996 Fiscal Year Final Research Report Summary
ISOLATION AND CHARACTERIZATION OF CYTOKINES SECRETED FROM CARDIAC MYXOMAS AND APPLICATION FOR CONGESTIVE HEART FAILURE
| Project/Area Number |
07670752
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | GUNMA UNIVERSITY |
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Principal Investigator |
KANDA Tsugiyasu GUNMA UNIVERSITY SCHOOL FROM MEDICINE,DEPARTMENT OF LABORATORY MEDICINE,INSTRUCTOR., 医学部, 助手 (40261838)
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Isao GUNMA UNIVERSITY SCHOOL OF MEDICINE,DEPARTMENT OF LABORATORY MEDICENE,PROFESSOR., 医学部, 教授 (50008273)
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| Project Period (FY) |
1995 – 1996
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| Keywords | interleukin-6 / interleukin-8 / cardiac myxoma / myocarditis / ウイルス |
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| Research Abstract |
We investigated the structure and charactrization of cytokines secreted from cardiac myxomas and analyzed the role of these cytokines for cangestive heart failure. Among eighteen cases of cardiac myxomas, 15 cases (83%) wass positively stained with antiinterleukin-6 (IL-6) antibody. Patients with IL-6 positive cardiac myxomas showed autoimmune like symptoms, such as weghtoloss, easy fatigability and fever. Their laboratory findings revealed higher levels of immunoglobulin G and A or gamma-globulin. Cytokines secreted from cardiac myxoma was analyzed by Western blotting. The molecular weight was about 10 to 12 kDa. This band was positive with anti-interleukin-8 (IL-8) antibodies.
Cultured supernatants obtained from cardiac myxomas inproved the survival of mice infected with encephalomyocarditis virus (EMCV). We compared the effects for viral myocarditis inbetween IL-6 and IL-8. IL-6, not IL-8, was beneficial for viral myocarditis of mice. Histopathological examination and viral analysis showed the reduction of myocarial necrosis by IL-6.
Therefore, we hypohtesize that IL-6 secreted from cardiac myxoma induces autoimmune like symptoms in patients with cardiac myxoma and that IL-6 is beneficial for the reduction of cardiac myxomas of mice induced from EMCV.
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