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1997 Fiscal Year Final Research Report Summary

STUDY OF THE REGURATORY MECHANISM OF CUTANEOUS HERPES SIMPLEX VIRUS(HSV)INFECTION

Research Project

Project/Area Number 07670956
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Dermatology
Research InstitutionSAPPORO MEDICAL UNIVERSITY

Principal Investigator

MIURA Shunsuke  SAPPORO MEDICAL UNIVERSITY,DEPARTMENT OF DERMATOLOGY,ASSISTANT PROFESSOR, 医学部, 講師 (50244364)

Co-Investigator(Kenkyū-buntansha) FUJII Nobuhiro  SAPPORO MEDICAL UNIVERSITY,DEPARTMENT OF MICROBIOLOGY,PROFESSOR, 医学部, 教授 (90133719)
Project Period (FY) 1995 – 1997
KeywordsHERPES SIMPLEX VIRUS / PERSISTENT INFECTION / HUMAN KERATINOCYTE / NEURAL CELLS / INTERFERON / CELL DEATH / LATENT INFECTION / HSV
Research Abstract

1)Human skin SCC-derived HSC-1 cells were infected with human herpesvirus-1(HSV), the strain HSV-PH,at the multiplicity of infection (MOI) of 10^6Using the indirect immunofluorescent technuiques, HSV was positive up to 6 weeks. This suggested that HSC-1 cells, which is keratinocyte lineage, can harbor HSV for up to 6 weeks without morphologic change.
2)HSC-1 cells and retinoic acid treated NT-2 cells were infected with HSV-PH strain at the MOI of 10^<-7> and 10^<-8>. NT-2 cells did not show any cytopathic effect (CPE) and detached same as mock-infected cells when they became confluent. On the other hand, HSC-1 cells showed CPE at the time of being confluent. These data suggested the difference of infection pattern at the low MOI between keratinocytes and neuron oriented cells, and responses of keratinocytes to HSV infection was dependent on MOI.
3)Titer of the supernatant of neural cells and keratinocytes infected with HSV-1 at the various MOI (10^<-2>-10^<-6>) were assayd using Vero cells. Neural cells showed higher CPE than keratinocytes at the MOI between 10^<-2>-10^<-5>. In any cell type, no cells showed CPE at the MOI of 10^<-6>.
4)Considering these results 1)-3), we concluded rhat epidermal keratinocytes showed active infection when large quantity of HSV appeared in the skin, but a few amount of virus did not induce apparent change to infected cells and stayd there for several weeks.

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Shunsuke Miura: "Ultraviolet light and Immunity to Coelenterate Venom" CUTIS. 57.No.4. 201-204 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nobuhiro Fujii: "Investigation of IFN Type-1 Receptor and IFN Regulatory Factor Expression Relating to Induction of 2',5'-Oligoadenylate Synthease in Cells Persistently Infected with The Mumps Virus." Microbiol.Immunol.40(10). 777-781 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tomokazu Indoh: "Augmentation of Interferon Production after Cell-Differentiation of ug37 cells by TPA" Microbiol.Immunol.40(9). 675-679 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Noriko Yokosawa: "Suppressed Induction of 2',5',As' in Cells with HTLV-1 Is Not Associated with the Flunctuation of IFN-Receptor,IFN-Regulatory Factors(IRF-1 and IRF-2)or STAT-1α" Microbiol.Immunol.41(1). 63-66 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shunsuke Miura, Joseph W.BUrnett, and Laure Aurelian.: "Ultraviolet Light and Immunity to Coelenterate Venom." Cutis. 57. 201-204 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nobuhiro Fujii, Noriko Yokozawa, Setsuko Ishida, Sachiko Shirakawa, Toru Kubota, Tomokazu Indou, Kei Fujinaga, and Teruo Yashiki: "Investigation of IFN Type-1 Receptor and IFN Regulatory Factor Expression Relating to Induction of 2', 5'-Oligoadenylate Synthetase in Cells Persistently Infected with the Mumps Virus" Microbiol.Immunol. 40(10). 777-781 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tomokazu Indoh, Sachiko Shirakawa, Toru Kubota, Teruo Yashiki, Emiko Isogai, and Nobuhiro Fujii: "Augmentation of Interferon Production after Cell-Differentiation of U937 Cells by TPA." Microbiol.Immunol.40(9). 675-679 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noriko Yokosawa, Tomokazu Indoh, Toru Kubota, Teruo Yashiki and Nobuhiro Fujii: "Suppressed Induction of 2-5AS in Cells with HTLV-1 Is Not associated with the Fluctuation of IFN-Receptor, IFN Regulatory Factors (IRF-1 and IRF-2) or STAT-1 alfa." Microbiol.Immunol.41(1). 63-66 (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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