1996 Fiscal Year Final Research Report Summary
Cell kinetics in cultured tumor cells aftertreatment with radiation and chemotherapy
| Project/Area Number |
07671034
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | Kansai Medical University |
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Principal Investigator |
AKAGI Kiyoshi KANSAI MEDICAL UNIVERSITY,FUCULTY OF MEDICINE,ASSISTANT PROFESSOR, 医学部, 講師 (30098115)
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| Co-Investigator(Kenkyū-buntansha) |
ITAGAKI Yasushi KANSAI MEDICAL UNIVERSITY,FUCULTY OF MEDICINE,ASSISTANT PROFESSOR, 医学部, 助手 (00193409)
HARIMA Keizou KANSAI MEDICAL UNIVERSITY,FUCULTY OF MEDICINE,ASSISTANT PROFESSOR, 医学部, 講師 (20121973)
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| Project Period (FY) |
1995 – 1996
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| Keywords | Irradiation / Experimental Tumor / Cell Cycle |
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| Research Abstract |
The cell cycle was analyzed using double staining with an anti-bromodeoxyuridine (BrDU) monoclonal anti-body and propidium iodide (PI). Changes in cell kinetics after irradiation were compared with those seen by the conventional PI-based DNA histogram method. The effect of irradiation on cell kinetics has been studied primarily by counting G2 arrested cells. By the present BrdU method, a rapid tradition, from G1 to S-phase was observed within 2h of irradiation, followed by G1 blook. Cell in the S-phase progressed to G2+M where they arrested, resulting in a decreased percentage of S cells, (<5%). Release of G1 after>18h. The initial Glarrest induced by irradiation was confirmed for the first time by the present BrdU-PI double atsining.
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