1996 Fiscal Year Final Research Report Summary
Analysis for the expression and localization of PKC isoform in colorectal cancer
| Project/Area Number |
07671326
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | KITASATO UNIVERSITY |
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Principal Investigator |
KURANAMI Masaru Kitasato Univ.Schl.of Medicine, Surgery, Assistant Professor, 医学部, 講師 (80170075)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Kenji Kitasato Univ.Schl.of Medicine, Surgery, Research Associate, 医学部, 助手 (10220881)
KIKUCHI Shiro Kitasato Univ.Schl.of Medicine, Surgery, Assistant Professor, 医学部, 講師 (30161417)
TSUKAMOTO Hideto Kitasato Univ.Schl.of Medicine, Surgery, Assistant Professor, 医学部, 講師 (60146420)
KAKITA Akira Kitasato Univ.Schl.of Medicine, Surgery, Professor, 医学部, 教授 (90109439)
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| Project Period (FY) |
1995 – 1996
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| Keywords | Colorectal cancer / liver metastasis / PKC isoforms / Tumor bank / Immunohistochemistry / Rnase protection assay |
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| Research Abstract |
Protein kinase C (PKC), a serine/threonine kinase central to signal transduction, is implicated in tumor promotion. At present, 10PKC isoforms have been cloned but their precise tissue-specific role has yet to be defined. In order to determine it PKC is reduced in colorectal cancers (CRC) and if specific PKC isoforms are altered in human CRC progression, specific PKC isoform mRNA and protein expression were examined. We established tumor bank that involved 17 cases of colorectal cancer, one case of metastatic liver tumor and two cases of primary liver tumor. PKC-alpha, Bll, gamma, delta, eta (L), epsilon and zeta were expressed in all tissues. PKC-alpha, Bll, delta, eta (L), epsilon and zeta were decreased in most primary CRC.Alteration of the localization of PKC isoform protein is examined in PKC-beta and delta. PKC-beta could not be detected in cancer tissue. PKC-delta was expressed under sub-cellularmembrane in normal colonic mucosa although cancer showed diffuse staining. Since mRNA expression for most PKC isoforms is decreased in CRC,the previously reported decreases in overall PKC activity in CRC are not solely due to a post-translational enzyme modification. Since certain PKC isoforms were expressed uniquely different in CRC relative to normal colonic mucosa, our resutls suggest that specific PKC isoforms may be involved in human CRC progression.
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