1996 Fiscal Year Final Research Report Summary
Bystander killing effect in gynecological cancer.
| Project/Area Number |
07671781
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Osaka University |
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Principal Investigator |
SAMEJIMA Yoshihiro Osaka Univ.Med.School Dept.of Obstel.and Gyne.research assistant, 医学部, 助手 (30231351)
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| Co-Investigator(Kenkyū-buntansha) |
NOBUNAGA Toshikatsu Osaka Univ.Med.School Dept.of Obstel.and Gyne.research assistant, 医学部, 助手
AZUMA Chihiro Osaka Univ.Med.School Dept.of Obstel.and Gyne.research assistant, 医学部, 助手 (20151061)
SAJI Fumitaka Osaka Univ.Med.School Dept.of Obstel.and Gyne.associate professor, 医学部, 助教授 (90093418)
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| Project Period (FY) |
1995 – 1996
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| Keywords | gene therapy / endometrial cancer / herpes virus / cervical adenocarcinoma / thymidine kinase / ganciclovir / bystander killing effect |
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| Research Abstract |
1.Establishment of gynecological cancer cell lines expressing thymidine kinase derived from herpes virus.
We transfected three kinds of endometrial cancer cell lines, a kind of cervical adenocarcinoma cell line and two kinds of choriocarcinoma cell lines with the plasmid containing thymidine kinase gene derived from herpes virus (HSV-tk) and neomycin resistance (neo^r) gene by means of the liposome method. We obtained some cell clones expressing the neo^r gene by the selection with G418. As the second selection, we performed ganciclovir (GCV) -sensitivity test. Consequently we established two clones which highly sensitive to GCV derived from endometrial cell line, HHUA,and cervical adenocarcinoma cell line, BU25.
2.Bystander killing effect in gynecological cancer cell lines.
We co-cultured HSV-tk positive cells with their original cells in various ratios in the presence of GCV for several days. The number of surviving cells was determined by dye (trypan blue) exclusion method. The original cells, which are resistant to GCV,were killed by GCV only when they co-cultured with HSV-tk positive cells, indicating that bystander killing effect can occur also in uterine adenocarcinomas. This result suggests the possibility of the effective gene therapy of gynecological cancer by means of HSV-tk and GCV.
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