1997 Fiscal Year Final Research Report Summary
Studies onstructural analysisforglucose isomerase of Bifidobacterium adolescentis and its application for diagnostic reagent
| Project/Area Number |
07680052
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
家政学
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| Research Institution | CHUKYO WOMEN'S UNIVERSITY |
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Principal Investigator |
HORITSU Hiroyuki CHUKYO WOMEN'S UNIVERSITY HEALTH SCIENCE,NUTRITION SCIENCE,PROFESSOR, 健康科学部, 教授 (60021680)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIOKA Shigeko CHUKYO WOMEN'S UNIVERSITY HEALTH SCIENCE,NUTRITION SCIENCE,ASSOCIATE PROFESSOR, 健康科学部, 助教授 (50073581)
ETO Yoshiharu CHUKYO WOMEN'S UNIVERSITY HEALTH SCIENCE,NUTRITION SCIENCE,PROFESSOR, 健康科学部, 教授 (10073579)
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| Project Period (FY) |
1995 – 1997
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| Keywords | Glucoseisomerase / Bifidobacterium adolescentis / Glucose determination / Diagnostic reagent / 食品中の糖定量 |
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| Research Abstract |
Exact Glucose isomerase found originally by this author from Bifidobacterium adolescentis was purified by using succesively DE-32 cellulose chromatography, Hiprep. Sephacryl-S-300HR gel filtration and Mono-Q-HR5/5 column high performance liquid chromatography to about thirty-fold purification from cell free extract of this microorganismus. For application using this enzyme preparation, we could succeed for determination of glucose concentration in sera of many kinds of animals, human, cow, horse, sheep, pig, dog, chicken, cat, rat and mouse. And this enzyme method is also used for determination of glucose concentration in foods, for example, soft drinks and Miso. Accuracy of glucose determination due to glucose isomerase was checked by authentic glucose oxidase method using as diagnostic test. As the results, it was found that our method was also accurate as well as glucose oxidase method.
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