1997 Fiscal Year Final Research Report Summary
Expression and function of structural proteins of paramyxovirus transcription complex
| Project/Area Number |
07680736
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Kinki University (1996-1997)
Mie University (1995) |
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Principal Investigator |
MATSUMURA Haruo Kinki University School of Medicine, Department of Immunology, Assistant professor, 医学部, 助手 (10229536)
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| Project Period (FY) |
1995 – 1997
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| Keywords | RNA editing / polymerase / transcriotion / replication / phosphoprotein / baculovirus / nucleocapsid |
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| Research Abstract |
V gene of human parainfluenza virus type 2 (hPIV2) , a member of paramyxoviruses, is transcribed into both a V and a P mRNA.The V mRNA is a faithful transcript of the V gene, however the P mRNA is transcribed by RNA editing mechanism in hPIV2-infected mammalian cells. Thus both the P and the V proteins are translated in the cells. We constructed recombinant baculoviruses containing the original V gene, which has seven G residues at its editing site and a manipulated V gene, which has ten G residues at its editing site. Unexpectedly, a small amount of the P protein was synthesized in addition to the V protein when the V gene was expressed in the recombinant baculovirus-infected insect cells. Furthermore, synthesis of the P protein increased when a recombinant baculovirus containing a V gene, which has extended length of G residue atretch, that is ten G residues, at its editing site, was expressed in the insect cells. Both the P and the V proteins were synthesized in in vitro translation of mRNA from the recombinant baculovirus-infected cells. Moreover, both G-residue insertions and a deletion were detected in mRNA.While the P protein was not detected in in vitro translation of the V RNA that was transcribed in vitro by T7 RNA polymerase. These results suggest that the non-templated G residues were inserted and deleted at the transcription level without paramyxovirus L protein.
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