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1997 Fiscal Year Final Research Report Summary

PHYSIOLOGICAL AND BIOCHEMICAL STUDIES ON SEX DIFFERENTIATION OF SILKWORMS

Research Project

Project/Area Number 07806008
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 蚕糸・昆虫利用学
Research InstitutionSHINSHU UNIVERSITY

Principal Investigator

KAJIURA Zenta  SHINSHU UNIVERSITY,TEXTILE SCIENCE AND TECHNOLOGY,ASSISTANT PROFESSOR, 繊維学部, 助手 (10224403)

Co-Investigator(Kenkyū-buntansha) NAKAGAKI Masao  SHINSHU UNIVERSITY,TEXTILE SCIENCE AND TECHNOLOGY,ASSOCIATED PROFESSOR, 繊維学部, 助教授 (70135169)
TAKEI Ryuzo  SHINSHU UNIVERSITY,TEXTILE SCIENCE AND TECHNOLOGY,PROFESSOR, 繊維学部, 教授 (80021161)
Project Period (FY) 1995 – 1997
KeywordsBombyx mori / Antheraea yamamai / Antheraea pernyi / vitellogenin / female-specific fat body protein / glutathione S-transferase / colleterial gland
Research Abstract

Using 3mug of total RNA prepared from the fat bodies of mature larvae, the first strand cDNA was synthesized with reverse transcriptase and oligo dT_<18> primer as a template for PCR.PCR was performed in the standard condition with Ex Taq DNA polymerase and synthetic oligonucleotides as primers.
The resulting PCR products were separated on a 1% agarose gel, and a 0.85kb fragment was obtained using sense primer and oligo dT_<18>. The amplified DNA fragment was used as the template in the second PCR with sense Kpn I-primer and antisense primer under the same condition. The resulting 0.85 kb fragment was excised from the agarose gel, purified, subcloned into pUC118 and sequenced.
The cloned cDNA covered N-terminal region, a putative polyadenylation signal (AATAAA), and poly(A). The N-terminal amino-acid sequence decided by Edman method matched completely ot the deduced amino-acid sequence of 24K.The 24KcDNAs of Antheraea pernyi and Antheraea yamamai showed highly conservation. Homology search shows the low identity between 24K and glutathione S-transferase.

  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] 梶浦 善太・大島 猛・中垣 雅雄 武井 隆三: "野蚕の雌附属腺(膠質腺)の組織学的観察と分泌タンパク質" 日本蚕糸学雑誌. 66. 413-419 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Zenta Kajiura, Marcia Noriko Ykoyama, Masao Nakagaki, Ryuzo Takei: "Purification,developmental profile and biosynthesis of arylphorin in the wild silk moth,Amtheraca pernyi" Applied Entomology and Zoology. 33(in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kajiura Z., Oshima T., Nakagaki M.and Takei R.: "Female accessory glands (colleterial glands) of the wild silkmoths and their secretory proteins" J.Seric.Sci.Jpn.66 (6). 413-419 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kajiura Z., Yokoyama M.N., Nakagaki M.and Takei R.: "Purification, developmental profile and biosynthesis of arylphorin in the wild silkmoth, Antherase pernyi" Applied Entomol.Zool.33 (2) (In press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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