Studies on the phenotypic expression of embryonic cells from teleost

1997 Fiscal Year Final Research Report Summary

• Project/Area Number: 07806026
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: KYOTO UNIVERSITY
• Principal Investigator: SEIKAI Tadahisa KYOTO UNIVERSITY,Faculty of Agriculture, Fisheries Research Station, Assistant, 農学部, 助手 (10144338)
• Project Period (FY): 1995 – 1997
• Keywords: Embryonic cells / Cell culture / Phenotypic expression / Japanese flounder / Pigment cell

Research Abstract

The objectives of this study is to establish the procedure of embryo cell from flatfish to reveal the control system in their differentiation process under culture conditions. The recording system with time laps video for cell culture was developed as the first step. Then, the embryos of Japanese flounder Paralichthys olivaceus were mainly used for this study, but those from red sea bream Pagrus major and black sea bream Acanthopagrus schlegeli were also succeeded in cell culture. The procedure of cell culture from developing embryos which was developed in this study was extremely easy and effective. The best medium for embryo cell culture was L-15 supplemented FBS.But this medium is not suitable to analyze the control factors of cell differentiation. Therefor, it is needed to develop the procedures using the medium without serum in near future.
From molura embryos, pigmet cells, nerve cells, epithelial cells and fibroblastic cells differentiated. Especially, embryos after neurula stage, variation of differentiated cells increased, and remarkable cell adhesions occurred. These clusters were networked by nerve cells. Pigment cells differentiated successively in such cell cluster with two different appearance in timing after plating corresponding in vivo development in Japanese flounder.