Function and regulation of stretch activated cation channel in osteoblast.

1997 Fiscal Year Final Research Report Summary

• Project/Area Number: 07807138
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Shiga University of Medical Science
• Principal Investigator: SARUHASHI Yasuo Shiga University of Medical Science, Department of Orthopaedic surgery, instrucor, 医学部, 助手 (40273403)
• Project Period (FY): 1995 – 1997
• Keywords: stretch activated channel / single channel / patch clamp / osteoblast

Research Abstract

A clonal cell line, HuO9, was recently established from a human osteosarcoma (Kawai1A.Olin Orthop Res , 1990,259,256-267). HuO9 cells are useful for studies on characteristics of human osteoblasts because HuO9 cells retain a high activity of alkaline phosphatase and secreted osteocalcin. We identified the potassium channel in HuO9 cells cultured for 2-14 days by patch-clamp, single-channel-recording techniques. So far the following properties were observed. Open probability of K+ channel (122 pS) depended upon membrane potentials and Ca concentration of bath solutions. Depolarization and high Ca concentration activated the K+ channel. Applied negative pressure to pipette also increased the open probability of the channel. jpn. J.Physiol. , VoI.48.Suppl. 1998