1997 Fiscal Year Annual Research Report

極東におけるライム病の疫学的調査、並びに伝播機序の解析

Research Project

Project/Area Number	08041181
Research Institution	University of Shizuoka
Principal Investigator	増澤俊幸静岡県立大学, 薬学部, 助教授 (10181645)
Co-Investigator(Kenkyū-buntansha)	朴慶煕建国大学, 医学部(韓国), 教授柳原保武静岡県立大学, 薬学部, 教授 (30046255) 中尾稔旭川医科大学, 医学部, 助手 (70155670) 高田伸弘福井医科大学, 医学部, 助教授 (90003409) 川端眞人神戸大学, 医学部, 教授 (30175294)
Keywords	ライム病 / ボレリア / マダニ / Borrelia burgderteri / Borrelia garinii / Borrelia afzelii / 系統分類 / 極東
Research Abstract	中国、韓国においてマダニ、野鼠の捕獲調査を実施し、これらからのボレリアの分離を行った。また、すでに得られていた極東ロシア分離株についても併せて分子生物学的性状の検討を行った。中国東北部、極東ロシアのシュルツェマダニ由来株は、Borrelia gariniiまたはB.afzeliiと同定された。また、B.gariniiでは欧州型とは別に日本に高頻度でみられる極東型が優占種であった。一方、韓国産タネガタマダニよりB.garinii、B.afzeliiの分離に初めて成功した。すでに韓国研究者によりタネガタマダニより分離されたB.afzelii関連群ボレリアの遺伝学的性状解析を行い、欧州のlxodes ricinus由来B.valaisiana、ならびに日本のハシブトマダニ由来Am501株に近縁であることを明らかにした。中国南部揚子江流域では野鼠Apodemus spp.とRattus spp.よりボレリアの分離に成功し、Apodemus spp.の一部は韓国にみられるB.afzelii関連群ボレリアであること、またRattus spp.由来株の多くは既知の種と異なり新種である可能性を示した。また、一個体のヤマトマダニより同じく新種と思われるボレリアの分離に成功した。現在、これらの株のより詳細な遺伝学的性状解析を進め、新種として記載する予定である。主要表層蛋白質A,B,Cの塩基配列に基づく系統解析を行い、日本と欧州間で極東ロシア、あるいは中国東北部を介してB.gariniiとB.afzeliiが伝播したことを示唆する結果を得た。

Research Products

(18 results)

All Other

All Publications (18 results)

[Publications] Toshiyuki Masuzawa et al.: "Presence of common antigenic epitope in outer surface protein (Osp) A and OspB of Japanese isolates identified as Borrelia garinii." Microbiol.Immunol.40. 455-458 (1996)
[Publications] Toshiyuki Masuzawa et al.: "Comparison of OspA serotypes for Borrelia burgdorferi sensu lato from Japan,Europe and North America." Microbiol.Immunol.40. 539-545 (1996)
[Publications] Toshiyuki Masuzawa: "Characterization of Borrelia sp.liolated from Ixodes tanuki,I.turdus,and I.columnae in Japanby restriction fragment length polymorphism of rrf(5S)-rrl(23S) intergenic spacer amplicons." FEMS Microbiol.Lett.,. 142. 77-83 (1996)
[Publications] Toshiyuki Masuzawa et al.: "Characterization of Borrelia spp.isolated from tick,Ixodes tanuki and small rodents in Japan." J.Wildl.Dis.32. 565-571 (1996)
[Publications] Yukita Sato et al.: "Prevalence of the Lyme disease spirochetes in Ixodes persulcatus and wild rodents in far eastern Russia." Appl.Environ.Microbiol. 62. 3887-3889 (1996)
[Publications] Toshiyuki Masuzawa et al.: "PCR-restriction fragment length polymorphism analysis of the ospC gene for detection of mixed culture and for epidemiological typing of borrelia burgdorferi sensu stricto." Clin.Diag.Lab.Immunol.4. 60-63 (1997)
[Publications] Hiroshi Fujita et al.: "A case of Lyme disease diagnosed serologically." J.Dermatol.24. 202-204 (1997)
[Publications] Jianhui Wang et al.: "Consensus sequence on the genes encoding the major outer surface proteins (OspA and OspB) of Borrelia garinii isolate." Microbiol. Immunol.41. 83-91 (1997)
[Publications] Kazuhide Kaneda et al.: "Glycosphingolipid-binding protein of Borrelia burgdorferi sensu lato." Infect.Immun.65. 3180-3185 (1997)
[Publications] Toshiyuki Masuzawa et al.: "Genetic diversity of Borrelia burgdorferi sensu lato isolated in far eastern Russia." Microbiol.Immunol.41. 595-600 (1997)
[Publications] Jianhui Wang et al.: "Characterization of Borrelia garinii isolated from patient in Hokkaido,Japan,by sequence analysis of OspA and OspB genes." Microbiol.Immunol.41. 673-679 (1997)
[Publications] Toshiyuki Masuzawa et al.: "Negative finding in cross-protective activity of Japanese Borrelia isolates against infection with three species of Lyme disease Borrelia in outbred mice." Microbiol.Immunol.41. 733-736 (1997)
[Publications] Toshiyuki Masuzawa et al.: "Homogeneity of Borrelia japonica and heterogeneity of Borrelia afzelii and “Borrelia tanukii"isolated in Japan,determined from ospC gene sequences." FEMS Microbiol.Lett.153. 287-293 (1997)
[Publications] Jianhui Wang et al.: "Sequence analysis of OspA and OspB genes of Borrelia garinii isolated from patient in Japan and its evolutionary implications." FEMS Microbiol.Lett.154. 371-375 (1997)
[Publications] Jianhui Wang et al.: "An unusual illegitimate recombinetion occurs in the linear plasmid encoded outer surface protein A gene of Borrelia afzelii." Microbiology. 143. 3819-3825 (1997)
[Publications] Kazuhide Kaneda et al.: "Infectivity and arthritis-induction of Borrelia japonica on SCID mice and immune competent mice : possible role of galactosylceramide binding activity on initiation of infection." Microbiol.Immunol.(印刷中).
[Publications] Nobuhiro Takada et al.: "Lyme disease spirochetes in ticks from northeastern China." J. Parasitol.(印刷中).
[Publications] Yasutake Yanagihara et al.: "FEMS Immunol.Med.Microbiol." Lyme disease(Lyme borreliosis), 12 (1997)

1997 Fiscal Year Annual Research Report

極東におけるライム病の疫学的調査、並びに伝播機序の解析

Principal Investigator

増澤 俊幸 静岡県立大学, 薬学部, 助教授 (10181645)

Research Products

[Publications] Toshiyuki Masuzawa et al.: "Presence of common antigenic epitope in outer surface protein (Osp) A and OspB of Japanese isolates identified as Borrelia garinii." Microbiol.Immunol.40. 455-458 (1996)

[Publications] Toshiyuki Masuzawa et al.: "Comparison of OspA serotypes for Borrelia burgdorferi sensu lato from Japan,Europe and North America." Microbiol.Immunol.40. 539-545 (1996)

[Publications] Toshiyuki Masuzawa: "Characterization of Borrelia sp.liolated from Ixodes tanuki,I.turdus,and I.columnae in Japanby restriction fragment length polymorphism of rrf(5S)-rrl(23S) intergenic spacer amplicons." FEMS Microbiol.Lett.,. 142. 77-83 (1996)

[Publications] Toshiyuki Masuzawa et al.: "Characterization of Borrelia spp.isolated from tick,Ixodes tanuki and small rodents in Japan." J.Wildl.Dis.32. 565-571 (1996)

[Publications] Yukita Sato et al.: "Prevalence of the Lyme disease spirochetes in Ixodes persulcatus and wild rodents in far eastern Russia." Appl.Environ.Microbiol. 62. 3887-3889 (1996)

[Publications] Toshiyuki Masuzawa et al.: "PCR-restriction fragment length polymorphism analysis of the ospC gene for detection of mixed culture and for epidemiological typing of borrelia burgdorferi sensu stricto." Clin.Diag.Lab.Immunol.4. 60-63 (1997)

[Publications] Hiroshi Fujita et al.: "A case of Lyme disease diagnosed serologically." J.Dermatol.24. 202-204 (1997)

[Publications] Jianhui Wang et al.: "Consensus sequence on the genes encoding the major outer surface proteins (OspA and OspB) of Borrelia garinii isolate." Microbiol. Immunol.41. 83-91 (1997)

[Publications] Kazuhide Kaneda et al.: "Glycosphingolipid-binding protein of Borrelia burgdorferi sensu lato." Infect.Immun.65. 3180-3185 (1997)

[Publications] Toshiyuki Masuzawa et al.: "Genetic diversity of Borrelia burgdorferi sensu lato isolated in far eastern Russia." Microbiol.Immunol.41. 595-600 (1997)

[Publications] Jianhui Wang et al.: "Characterization of Borrelia garinii isolated from patient in Hokkaido,Japan,by sequence analysis of OspA and OspB genes." Microbiol.Immunol.41. 673-679 (1997)

[Publications] Toshiyuki Masuzawa et al.: "Negative finding in cross-protective activity of Japanese Borrelia isolates against infection with three species of Lyme disease Borrelia in outbred mice." Microbiol.Immunol.41. 733-736 (1997)

[Publications] Toshiyuki Masuzawa et al.: "Homogeneity of Borrelia japonica and heterogeneity of Borrelia afzelii and “Borrelia tanukii"isolated in Japan,determined from ospC gene sequences." FEMS Microbiol.Lett.153. 287-293 (1997)

[Publications] Jianhui Wang et al.: "Sequence analysis of OspA and OspB genes of Borrelia garinii isolated from patient in Japan and its evolutionary implications." FEMS Microbiol.Lett.154. 371-375 (1997)

[Publications] Jianhui Wang et al.: "An unusual illegitimate recombinetion occurs in the linear plasmid encoded outer surface protein A gene of Borrelia afzelii." Microbiology. 143. 3819-3825 (1997)

[Publications] Kazuhide Kaneda et al.: "Infectivity and arthritis-induction of Borrelia japonica on SCID mice and immune competent mice : possible role of galactosylceramide binding activity on initiation of infection." Microbiol.Immunol.(印刷中).

[Publications] Nobuhiro Takada et al.: "Lyme disease spirochetes in ticks from northeastern China." J. Parasitol.(印刷中).

[Publications] Yasutake Yanagihara et al.: "FEMS Immunol.Med.Microbiol." Lyme disease(Lyme borreliosis), 12 (1997)

増澤俊幸静岡県立大学, 薬学部, 助教授 (10181645)