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1997 Fiscal Year Final Research Report Summary

EPIDEMIOLOGICAL STUDY ON LYME DISEASE IN THE FAR EASTERN COUNTRY

Research Project

Project/Area Number 08041181
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionField Research
Research Field Bacteriology (including Mycology)
Research InstitutionUniversity of Shizuoka

Principal Investigator

MASUZAWA Toshiyuki  University of Shizuoka, School of Pharmaceutical Sciences, Assosiate Professor, 薬学部, 助教授 (10181645)

Co-Investigator(Kenkyū-buntansha) 朴 慶熙  韓国, 建国大学・医学部, 教授
YANAGIHARA Yasutake  University of Shizuoka, School of Pharmaceutical sciences, Professor, 薬学部, 教授 (30046255)
NAKAO Minoru  Asahikawa Medical College, Research Associate, 医学部, 助手 (70155670)
TAKADA Nobuhiro  Fukui Medical College, Associate Professor, 医学部, 助教授 (90003409)
KAWABATA Masato  Kobe University, School of Medicine, Professor, 医学部, 教授 (30175294)
PARK Kyung Hee  Kon-Kuk University, School of Medicine, Professor
Project Period (FY) 1996 – 1997
KeywordsLYME DISEASE / BORRELIA / IXODES / BORRELIA BURGDORFERI / BORRELIA GARINII / BORRELIA AFZELII / SYSTEMATIC CLASSIFICATION / FAR EAST
Research Abstract

We made Lyme disease borrelia survey of vector ticks and resevoir animals in Northern part of China and the Yangtze River valley, and Korea. Borrelia isolates from these sources and from far eastern Russia we got previously were analyzed by 5S-23S rRNA intergenic spacer and flagellin gene PCR-RFLP analysis and sequence analysis.
Isolates from Ixodes persulcatus collected Northern China and far eastern Russia were identified as B.afzelii or B.garinii. B.garinii generate RFLP pattern C which is commonly found in Japan but not in Europe were dominant species in these area. Furthermore, isolates from I.nipponensis collected in central Korea were identified as B.garinii of far eastern type or B.afzelii. Isolates from I.nipponensis tentatively described as B.afzelii-related group obtained from Korean researcher were characterized on intergenic spacer and 16SrRNA sequences. These isolates showed relatively higher similarity values to B.valaisiana isolated from I.ricinus in Europe and strain Am501 from I.columnae in Japan. Isolates from Apodemus spp.and Rattus spp.captured in the Yangtze River valley were identified as B.afzelii-related group and new genotype, respectively. Also, one isolate from I.ovatus in the area showed the same feature to those from the Rattus spp. Further study to described new species is undertaken.
By the phylogenetic analysis based on the sequence of outer surface protein A,B,and C,genes, transmission of B.garinii and B.afzelii between Japan and Europe via far eastern country, such as far east Russia, northern China, and Korea was suggested.

  • Research Products

    (37 results)

All Other

All Publications (37 results)

  • [Publications] Toshiyuki Masuzawa et al.: "Presence of common antigenic epitope in outer surface protein(Osp)A and OspB of Japanese isolates identified as Borrelia garinii." Microbiol.Immunol.40. 455-458 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshiyuki Masuzawa et al.: "Comparison of OspA serotypes for Borrelia burgdorferi sensu lato from Japan,Europe and North America." Microbiol.Immunol.40. 539-545 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshiyuki Masuzawa: "Characterization of Borrelia sp.Iiolated from Ixodes tanuki,I.turdus,and I.columnae in Japan by restriction fragment length polymorphism of rrf(5S)-rrl(23S)intergenic spacer amplicons." FEMS Microbiol.Lett.142. 77-83 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshiyuki Masuzawa et al.: "Characterization of Borrelia spp.isolated from tick,Ixodes tanuki and small rodents in Japan." J.Wildl.Dis.32. 565-571 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yukita Sato et al.: "Prevalence of the Lyme disease spirochetes in Ixodes persulcatus and wild rodents in far eastern Russia." Appl.Environ.Microbiol.62. 3887-3889 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshiyuki Masuzawa et al.: "PCR-restriction fragment length poylmorphism analysis of the ospC gene for detection of mixed culture and for epidemiological typing of Borrelia burgdorferi sensu stricto." Clin. Diag.Lab.Immunol.4. 60-63 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiroshi Fujita et al.: "A case of Lyme disease diagnosed serologically." J.Dermatol.24. 202-204 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Jianhui Wang et al.: "Consensus sequence on the genes encoding the major outer surface proteins(OspA and OspB)of Borrelia garinii isolate." Microbiol.Immunol.41. 83-91 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kazuhide Kaneda et al.: "Glycosphingolipid-binding protein of Borrelia burgdorferi sensu lato." Infect.Immun.65. 3180-3185 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshiyuki Masuzawa et al.: "Genetic diversity of Borrelia burgdorferi sensu lato isolated in far eastern Russia." Microbiol.Immunol.41. 595-600 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Jianhui Wang et al.: "Characterization of Borrelia garinii isolated from patient in Hokkaido,Japan,by sequence analysis of OspA and OspB genes." Microbiol.Immunol. 41. 673-679 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshiyuki Masuzawa et al.: "Negative finding in cross-protective activity of Japanese Borrelia isolates against infection with three species of Lyme disease Borrelia in outbred mice." Microbiol.Immunol.41. 733-736 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshiyuki Masuzawa et al.: "Homogeneity of Borrelia japonica and heterogeneity of Borrelia afzelii and“Borrelia tanukii"isolated in Japan,determined from ospC gene sequences." FEMS Microbiol.Lett.153. 287-293 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Jianhui Wang et al.: "Sequence analysis of OspA and OspB genes of Borrelia garinii isolated from patient in Japan and its evolutionary implications." FEMS Microbiol.Lett.154. 371-375 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Jianhui Wang et al.: "An unusual illegitimate recombination occurs in the linear plasmid encoded outer surface protein A gene of Borrelia afzelii." Microbiology. 143. 3819-3825 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kazuhide Kaneda et al.: "Infectivity and arthritis-induction of Borrelia japonica on SCID mice and immune competent mice:possible role of galactosylceramide binding activity on initiation of infection." Microbiol.Immunol.(印刷中).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nobuhiko Takada et al.: "Lyme disease spirochetes in ticks from northeastern China." J.Parasitol.(印刷中).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yasutake Yanagihara et al.: "FEMS Immunol.Med.Microbiol." Lyme disease(Lyme borreliosis), 12 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshiyuki Masuzawas, Kazuhide Kaneda, Hiroyuki Suzuki, Jianhui Wang, Kazuto Yamada, Hiroki Kawabata, Russell C.Johnson, and Yasutake Yanagihara.: "Presence of common antigenic epitope in outer surface protein (Osp) A and OspB of Japanese isolates identified as Borrelia garinii." Microbiol.Immunol.40. 455-458 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toshiyuki Masuzawa, Bettina Wilske, Tetsuro Komikado, Hiroyuki Suzuki, Hiroki Kawabata, Nanao Sato, Koichi Muramatsu, Nobutake Sato, Emiko Isogai, Hiroshi Isogai, Russell C.Johnson, and Yasutake Yanagihara.: "Comparison of OspA serotypes for Borrelia burgdorferi sensu lato from Japan, Europe and North America." Microbiol.Immunol.40. 539-545 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toshiyuki Masuzawa, Tetsuro komikado, Atue Iwaki, Hiroyuki Suzuki, Kazuhide Kaneda, and Yasutake Yanagihara.: "Characterization of Borrelia sp.Iiolated from Ixodes tanuki, I.turdus, and I.columnae in Japan by restriction fragment length polymorphism of rrf (5S)-rrl (23S) intergenic spacer amplicons." FEMS Microbiol.Lett.142. 77-83 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toshiyuki Masuzawa, Hiroyuki Suzuki, Hiroki Kawabata, Fubito Ishiguro, Nobuhiro Takada, and Yasutake Yanagihara: "Characterization of Borrelia spp.isolated from tick, Ixodes tanuki and small rodents in Japan." J.Wildl.Dis.32. 565-571 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yukita Satop, Kenji Miyamoto, Atsue Iwaki, Toshiyuki Masuzaws, Yasutake Yanagihara, Edward I.Korenberg, N.B.Gorelova, Vladimir I.Volkov, Leonid I.Ivanov, and Rita.N.Liverova.: "Prefalence of the Lyme disease spirochetes in Ixodes persulcatus and wild rodents in far eastern Russia." Appl.Environ.Microbiol.62. 3887-3889 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toshiyuki Masuzawa, Tetsuro Komikado, and Yasutake Yanagihara: "PCR-restriction fragment length polymorphism analysis of the ospC gene for detection of mixed culture and for epidemiological typing of Borrelia burgdorferi sensu stricto." Clin.Diag.Lab.Immunol.4. 60-63 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiroshi Fujita, Koji Ogusu, Shunsuke Imaizumi, Toshiyuki Masuzawa, and Yasutake Yanagihara.: "A case of Lyme disease diagnosed serologically." J.Dermatol.24. 202-204 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Jianhui Wang, Toshiyuki Masuzawa, Tetsuro komikado, and Yasutake Yanagihara.: "Consensus sequence on the genes encoding the major outer surface proteins (OspA and OspB) of Borrelia garnii isolate." Microbiol.Immunol.41. 83-91 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kazuhide Kaneda, Toshiyuki Masuzawa, Kitsuzo Yasugami, Takashi Suzuki, Yasuo Suzuki, and Yasutake Yanagihara.: "Glycosphingolipid-binding protein of Borrelia burgdorferi sensu lato." Infect.Immun.65. 3180-3185 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toshiyuki Masuzawa, Atsue Iwaki, Yukita Sato, Kenji Miyamoto, Edward I.Korenberg, and Yasutake Yanagihara: "Genetic diversity of Borrelia burgdorferi sensu lato isolated in far eastern Russia." Microbiol.Immunol.41. 595-600 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Jianhui Wang, Toshiyuki Masuzawa, Muqing Li, and Yasutake Yanagihara.: "Characterization of Borrelia garinii isolated from patient in Hokkaido, Japan, by sequence analysis of OspA and OspB genes." Macrobiol.Immunol.41. 673-679 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toshiyuki Masuzawa, Toru Kurita, and Yasutake Yanagihara: "Negative finding in corss-protective activity of Japanese Borrelia isolates against infection with three species of Lyme disease Borrelia in outbread mice." Microbiol.Immunol.41. 733-736 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toshiyuki Masuzawa, Tetsuro komikado, Kazxuhide Kaneda, Takako Fukui, Katsuji Sawaki, and Yasutake Yanagihara: "Homogeneity of Borrelia japonica and heterogeneity of Borrelia afzelii and "Borrelia tanukii" isolated in Japan, determined from ospC gene sequences." FEMS Microbiol.Lett.153. 287-293 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Jianhui Wang, Toshiyuki Masuzawa, and Yasutake Yanagihara.: "Sequence analysis of OspA and OspB genes of Borrelia garinii isolated from patient in Japan and its evolutionary implications." FEMS Microbiol.Lett.154. 371-375 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Jianhui Wang, Toshiyuki Masuzawa, and Yasutake Yanagihara.: "An unusual illegitimate recombination occurs in the linear plasmid encoded outer surface protein A gene of Borrelia afzelii." Microbiology. 143. 3819-3825 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kazuhide Kaneda, Toshiyuki Masuzawa, Markus M.Simon, Emiko Isogai, Hiroshi Isogai, Kitsuzo Yasugami, Takashi Suzuki, Yasuo Suzuki, and Yasutake Yanagihara.: "Infectivity and arthritis-induction of Borrelia japonica on SCID mice and immune competent mice : possible role of galactosylceramide binding activity of initiation of infection." Microbiol.Immunol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nobuhiro Takada, Fubito ishiguro, Hiromi Fujita, Hai-Peng, Wang, Ji-Chun Wang, and Toshiyuki Masuzawa: "Lyme disease spirochetes in ticks from notrheastern China." J.Parasitol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yasutake Yanagihara, and Masuzawa: "Lyme disease (Lyme borreliosis)"

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] FEMS Immunol.Med.Microbiol.18. 249-261 (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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