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1997 Fiscal Year Final Research Report Summary

International collaboration of neuroblastoma

Research Project

Project/Area Number 08042002
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionSpecial Cancer Research
Research Field Pediatrics
Research InstitutionThe University of Tokyo

Principal Investigator

HAYASHI Yasuhide  University of Tokyo, Faculty of Medicine, Associate Professor, 医学部・附属病院, 講師 (30238133)

Co-Investigator(Kenkyū-buntansha) NAKAGAWARA Akira  Chiba Cancer Center, Chairman, 生化学, 部長
KOBAYASHI Miyuki  University of Tokyo, Faculty of Medicine, Asistant Professor, 医学部・附属病院, 助手 (60205391)
BESSHO Fumio  University of Tokyo, Faculty of Medicine, Associate Professor, 医学部・附属病院, 助教授 (40010285)
LOOK Thomas A  聖ユダ小児研究病院, 実験腫瘍, 教授
BRODEUR Garrett M.  Philadelphia Children's Hospital, Director, Professor, 教授
LOOK A. Thomas  St. Jude Children's Research Hospital, Director, Professor
Project Period (FY) 1996 – 1997
Keywordsneuroblastoma / N-myc gene / p16 gene / DCC gene / DPC4 gene / MADR2 gene / p73 gene / mass screening
Research Abstract

N-myc, p16, DCC, DPC4, MADR2 and p73 genes were analyzed in Japan, USA and Malaysia by Southern blotting, Northern blotting, Western blotting and PCR-single stand conformation polymorphism (SSCP). Loss of heterozygosity (LOH) in our Japanese study suggested high frequent LOH between D9S162 and IFNA on 9p, which included p16 gene. Homozygous deletion (HD) of p16 gene was not found in 81 Japanese samples (N-myc 6 samples). PCR-SSCP analysis identified only missense mutation, suggesting polymorphism. Absence or decreased expression of p16 gene was found in 6 of 10 Japanese cell lines and 6 of 9 USA cell lines, suggestings no significant difference between them. Hypermenthylation of the p16 gene was found in 6 of 10 Japanese cell lines and 6 of 9 USA cell lines, suggesting that hypermenthylation plays an important role for the development or progression of neuroblastoma. The commonly deleted region of 18q in neuroblastoma included DCC, DPC4 and MADR2 genes. Absence or reduced expression of DCC gene was found in half of the samples in 3 countries. PCR-SSCP analysis of DCC gene showed only missense mautation, suggesting polymorphism. Alterations of DPC4 and MDRR2 genes were relatively rare. These results suggested that DCC gene plays an important role in the dissemination of neuroblastoma, and that DPC4 and MAD2 gene are not involved in the development of neuroblastoma. Frequency of alterations of the p73 gene was not different among 3 countries. Our epidemiological study showed the frequency of the development of neuroblastoma increased (2 times) after mass screening. These results were found to be different from those in USA and Canada. Inetrnational Collaboration of neuroblastoma contributed to the pathogenesis of neuroblastoma in 3 countries.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Bessho F. et al.: "Effects of mass screening on age-specific incidence ofneuroblastoma"Int. J. Cancer. 67. 520-522 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Bessho F. et al.: "Where should neuroblastoma mass screening go?"Lancet. 348. 1672 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takita J. et al.: "Deletion map of Chromosome 9 and p16 (CDKN2A) gene alterations in neuroblastoma"Cancer Res.. 57. 907-912 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kong X-T et al.: "Expression and mutational analysis of the DCC, DPC4, and MADR2/JV18-1 genes in neuroblastoma"Cancer Res.. 57. 3772-3778 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakagawara A. et al.: "High levels of expression and nuclear localization of interleukin-1 beta converting Enzyme (ICE) and CPP32 in favorable human neuroblastomas"Cancer Res.. 57. 4578-4584 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamamoto K. et al.: "Spontaneous regression of localized neuroblastoma detected by mass screening"J. Clin Oncol. 16. 1265-1269 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Bessho F. et al.: "Effects of mass screening on age-specific incidence of neuroblastoma"Int. J. Cancer. 67. 520-522 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] F. Bessho: "Where should neuroblastoma mass screening go?"Lancet. 348. 1672 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takita J, Hayashi Y, Kohno T, Yamaguchi N, Hanada R, Yamamato K, Yokota J: "Deletion map of Chromosome 9 and p16(CDKN2A) gene alterations in neuroblastoma"Cance Res. 57. 907-912 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kong X-T, Choi Sh, Xu Feng, Chen T, Hayashi Y: "Expression and mutational analysis of the DCC, DPC4 and MADR2/JV18-1 genes in neuroblastoma"Cancer Res. 57. 3772-3778 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakagawara A, Nakamura Y, Ikeda H, Hiwasa T, Kuida K, Su MS, Zhao H, Cnaan A, Sakiyama S: "High levels of expression and nuclear localization of interleukin-1 beta converting Enzyme (ICE) and CPP32 in favorable human neuroblastomas"Cancer Res. 57. 4578-4584 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamamoto K, Hanada R, Kikuchi A, Ichikawa M, Aihara T, Oguma E, Moritani T, Shimanuki Y, Tanimura M, Hayashi Y: "Spontaneous regression of localized neuroblastoma detected by mass screening"J Clin Oncol. 16. 1265-1269 (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2001-10-23  

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