1997 Fiscal Year Final Research Report Summary
Molecular basis of cellular functions in Dictyostelium
| Project/Area Number |
08044189
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Joint Research |
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| Research Field |
Developmental biology
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| Research Institution | Graduate School of Science |
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Principal Investigator |
MAEDA Yasuo Tohoku University, Graduate School of Science, Professor, 理学研究科, 教授 (50025417)
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| Co-Investigator(Kenkyū-buntansha) |
VERON Michael Division of Biochemistry, Pasteur Institute, Professor, 生化学部, 教授
FIRTEL Richard A Center of Molecular Genetics, California University, Professor, サンジエゴ校・分子遺伝センター, 教授
WILLIAMS Jeffrey MRC Laboratory, University College of London, Professor, MRC研究所, 教授
SUTOH Kazuo University of Tokyo, Graduate School of Artsand Sicences, Professor, 大学院・総合文化研究科, 教授 (20111453)
TAKEUCHI Ikuo Okazaki National Research Institute, President (90025239)
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| Project Period (FY) |
1996 – 1997
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| Keywords | cell cycle / growth / differentiation / pattern formation / cytokinesis / chemotaxis / starvation / Dictyostelium / 細胞性粘菌 |
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| Research Abstract |
(1) Using the good method for cell synchnony and doubly transformed Dictyostelium cell populations, it has been demonstrated that there is a strong correlation between ceII cycle position at the time of starvation and subsequent expression of a marker of tip differentiation. CeIIs starved in very late-G2 phase selectively differentiated into tip prestalk cells, while cells starved in mid-late G2 phase into the posterior prespore cells. Thus a combination of cell cycle stage and positional information dictates cell fate.
(2) Nocodazole- or calyculin A-treated ceIIs, whose growth around the early aggregate (mound) stage was markedly inhibited, exhibited abnormal morphogenesis to form a stick-like multicellular structure, and prespore differentiation was greatly suppressed. Accordingly, the cell-cycle progression around the mound stage seems to be required for prespore differentiation.
(3) The signals for starvation response have been shown to be transduced through elevated [Ca^<2+>] i. Also, some phosphoproteins responsible for starvation response were found.
(4) Three genes ware isolated and identified as ones specifically expressed during the transition of Dictyostelium cells from growth to differentiation.
(5) Dictyostelium IQGAP-related protein has been shown to be specifically involved in the completion of cytokinesis. In addition, sliding of actin filaments was found to be greatly modulated by mutations of the SH2 cystcine in Dictyostelium myosin ll.
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[Publications] Adachi, H., Takahashi, Y., Hasebe, T., Shirouzu, M., Yokoyama, S.and Sutoh, K.: "Dictyostelium IQGAP-related protein specifically involved in the completion of cytokinesis" J.Cell Biol.137. 891-898 (1997)
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