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1998 Fiscal Year Final Research Report Summary

Molecular Genetics of Host-Specific Toxins in Plant Disease

Research Project

Project/Area Number 08044207
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research Field 植物保護
Research InstitutionTottori University

Principal Investigator

KOHMOTO Keisuke  Tottori University, Faculty of Agriculture, Professor, 農学部, 教授 (80032093)

Co-Investigator(Kenkyū-buntansha) ITOH Yasuo  Sinshu University, Faculty of Natural Science, Res.Associate, 理学部, 助手 (70283231)
AKIMITSU Kazuya  Kagawa University, Faculty of Agriculture, Assoc.Professor, 農学部, 助教授 (80263888)
TSUGE Takashi  Nagoya University, Graduate School of Life Science and Agriculture, Assoc, Profe, 大学院・生命農学研究科, 助教授 (30192644)
KODAMA Motoichiro  Tottori University, Faculty of Agriculture, Assist.Professor, 農学部, 講師 (00183343)
OHTANI Hiroshi  Tottori University, United Graduate School of Agricultural Sciences, Professor, 大学院・連合農学研究科, 教授 (50032305)
Project Period (FY) 1996 – 1998
KeywordsREMI / CPS gene / PKS gene / host-specific toxin / AMT / AKT / ACRS / proteome analysis
Research Abstract

Many fungal genes responsible for the producion of host-specific toxins were cloned from Alternaria and Cochliobolus pathogens. Some of them were elucidated thier structures and function in the course of US/Japan Joint Research Project. A cyclic peptide synthetase gene (4MT) for AM-toxin of leaf blotch of apple pathogen was found to bel3kb in length with no introns and have four domains of amino acid activation specific for the amino acids constituting the peptide toxin. The gene cluster involved in AK-toxin biosynthesis in the black spot pathogen of Japanese pear were made structural and functional analyses AKT1 (fatty acid synthesis), AKT3(modification of fatty acid), AKTR(regulator), AKTS1 (similar to threonine dehydratase, its true role is unclear, but specific to the Japanese pear pathotype) and AKT2 (no similarity to known proteins in the databases). The strawberry pathotye had AKTl AKT3 and AKTR homologues, and the tangerine pathotype possessed AKTR homologue. The shared distribution of the genes were compatible with the molecular similarity of toxins. Also, structure and function of TOX1 and TOX2 in Cochijobolus were elucidated in depth. A gene for the sensitivity to ACR-toxin was cloned from mtDNA of host rough lemon, and a protein specific to the AM-toxin sensitivity was isolated from susceptible apple leaves by proteome analysis. Most fungal genes for host specific toxin biosynthesis would be horizontally transferred.

  • Research Products

    (31 results)

All Other

All Publications (31 results)

  • [Publications] Akamatsu, H.,et al.: "Molecular karyotypes of Alternaria pathogens known to produce host-specific toxins" Current Genetics. in press. (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kaneko, I.,et al.: "Intracellular localization and expression of the plasmid PAAT56 in the Japanese pear pathotype of Alternaria alternata" Ann. Phytopathol. Soc. Jpn.65. 25-31 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kodama, M.,et al.: "The translocation-assicuated Toxl locus of Cochliobolus heterostrophus is two gonetic elements on two different chromosomes" Genetics. 151. 585-596 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakajima, H.,et al.: "Biosynthesis of sorokinianin a phytotoxin of Bipolaris sorokiniana : Evidence of mixed origin from the sesqiuterpene and TCA pathway" Tetrahedron Letters. 39. 1013-1016 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Christiansen, S.K.,et al.: "The two Cochliobolus mating type genes are conserved among species but one of them is missing in C.vuctorise" Mycological Research. 102. 919-929 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Otani, H.,et al.: "Production of a host-specific toxin by germinating spores of Alternaria brassicicola" Physiological and Molecular Plant Pathology. 52. 285-295 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Young, C.,et al.: "Paxilline-negative mutants of Penicillium paxilli generated by heterologous and homologous plasmid integration" Current Genetics. 33. 368-377 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miyashita, M.,et al.: "Fasile syntheses of AM-toxins and analogs as cyclic depsi-peptides by the solid-phase method" Bioscience, Biotechnology and Biochemistry. 62. 1799-1801 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akamatsu, H.et al.: "AAL-toxin deficient mutants of Alternaria alternata tomato pathotype by restriction engyme-mediated imtegration" Phytopathology. 87. 967-972 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kaneko, I.et al.: "Structural analysis of the plasmid pAAT56 of the filamentous fungus Alternaria alternata" Gene. 203. 51-57 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kusaba, M.et al.: "Mitochondrial DNA variation in host-Apecific toxin-producing pathogens in the genus Alternaria" Ann. Phytopathol. Soc. Jpn.63. 463-469 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Walton, J.D.: "Host. selective toxins : agents of compatibility" Plant Cell. 8. 1723-1733 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kohmoto, K.et al.: "Molecular Genetics of Host-Specific Toxins in Plant Disease" Kluwer Academic Publishers, Dordrecht, 413 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoder, O.C.et al.: "Cochliobolus spp. and thein host-specific toxins In : The Mycota, V-A PP. 145-166." Springer-Verlag, Berlin, 247 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akamatsu, H., Taga, M., Kodama, M., Johnson, D.R., Otani, H.and Kohmoto, K.: "Molecular karyotypes of Alternaria plant pathogens known to produce host-specific toxins." Current Genetics. (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kaneko, I., Owaki, M.and Tsuge, T.: "Intercellular localization and expression of the plasmid pAAT56 in the Japanese pear pathotype of Alternaria." Ann.Phytopathol.Soc.Jpn.65. 25-31 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kodama, M., Rose, M.S., Yang, G.Yun, S.H., Yoder, O.C.and Turgeon, B.G.: "The translocation-associated Tox1 locus of Cochliobolus heterostrophus is two genetic elements on two different chromosomes." Genetics. 151. 585-596 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakajima, H., Toratsu, Y., Ichinoe, M.and Hamasaki, T.: "Biosynthesis of Sorokininin a phytotoxin of Bipolaris sorokiniana : Evidence of mixed origin from the sesquiterpene and TCA pathways." Tetrahedron Letters. 39. 1013-1016 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Otani, H., Kohnobe, A., Kodama, M.and Kohmoto, K.: "Production of a host-specific toxin by germinating spores of Alternaria brassicicola." Physiological and Molecular Plant Pathology. 52. 285-295 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akamatsu, H., Itoh, Y., Kodama, M., Otani, H.and Kohmoto, K.: "AAL-toxin deficient mutants of Alternaria alternata tomato pathotype by restriction enzyme-mediated integration." Phytopathology. 87. 967-972 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Arase, S., Fukuyama, R., Honda, Y.and Nozu, M.: "Light-dependent induction Sekiguchi lesion by Magnaporthe grisea infections in rice cv.Sekiguchi-asahi." J.Phytopath.145. 31-35 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Isshiki, A., K.Akimitsu, N.Nishio, M.Tsukamato, and H.Yamamoto.: "Puri fication and characterization of an endopolygalacturonase from the rough lemon pathotype of Alternaria alternata, the cause of citrus brown spot disease" Physiological and Molecular Plant Pathology. 51. 155-167 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kaneko, I., Katsuya, S.and Tsuge, T.: "Structural analysis of the plasmid pAAT56 of the filamentous fungus Alternaria alternata" Gene. 203. 51-57 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Katsuya, S., Kaneko, I., Owaki, M., Ishikawa, K., Tsujimoto, T.and Tsuge, T.: "Circular DNA plasmid in the phytopathogenic fungus Alternaria alternata : its temperature-dependent curing and association with pathogenicity." Genetics. 146. 111-120 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kusuba, M.and Tsuge, T.: "Mitochondrial DNA variation in host-specific toxin-producing pathogens in the genus Alternaria" Ann.Phytopathol.Soc.Jpn.63. 463-469 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Uehara, T., Arase, S., Honda, Y., Park, P., and Nozu, M.: "Ultrastructural effects of Magnaporthe grisea toxin(s) on mitochondria of host and non-host plants." Ann.Phytopathol.Soc.Jpn.63. 69-77 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Itoh, Y.and Scott, B.: "Effect of de-phosphorylation of linearized pAN7-1 and of addition of restriction enzyme on plasmid integration in Penicilillium paxilli." Current Gentics. 32. 147-151 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Adachi, Y., Watanabe, H.and Tsuge, T.: "Relationships between genetic polymorphisms and fungicide resistance whithin Alternaria alternata" Phytopathology. 86. 1248-1254 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ahn, J.-H.and Walton, J.D.: "Chromozonal organization of TOX2, a complex locus requied for host-selective toxin biosynthesis in Cochliobolus carbonum." Plant Cell. 8. 887-897 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Walton, J.D.: "Host-selective toxins : agents of compatiibility." Plant Cell. 8. 1723-1733 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Kohmoto and O.C.Yoder, eds.: "Molecular Genetics of Host-Specific Toxins in Plant Disease." Kluwer Academic Publ.Dordrecht/Boston/Kondon. 413 (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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