| Co-Investigator(Kenkyū-buntansha) |
WOOTTEN Rodoney Stiring University, Institute of Aquaculture, Professor, 養殖研究所, 教授
YOSHIZAKI Goro Tokyo University of Fisheries, Faculty of Fisheries, Assistant Professor, 水産学部, 助手 (70281003)
HIRONO Ikuo Tokyo University of Fisheries, Faculty of Fisheries, Assistant Professor, 水産学部, 助手 (00270926)
OKAMOTO Nobuaki Tokyo University of Fisheries, Faculty of Fisheries, Professor, 水産学部, 教授 (40114912)
TAKASHIMA Fumio Tokyo University of Fisheries, Faculty of Fisheries, Professor, 水産学部, 教授 (60041703)
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| Research Abstract |
We have cloned, sequenced and partially characterized several biodefenese and immune *elated gene cDNA from cloned Japanese flounder. These cDNA were transferrin, complement component C3, C7, C8, and C9, complement regulatory factor, interferon regulator factor, T cell receptor alpha and delta, 1gM, IgD, Ig light chain, Ig kappa chain, antivirus protein Mx, Chemokine receptor, interferon inducible protein 56K, lysozyme, and so on.
We have developed an effective method to introduce foreign gene into fish and have exploited the active promoters in transgenic fish systems. The electroporation of hydrating sperm was very effective and the obtained transgenic rate was 3 times higher than the ordinal electroporation method. Also, we have showed that Medaka B-actin promoter was very active in most of the tissue ; however, trout vasa promoter was active only in germ cells in transgenic rainbow trout.
We made monoclonal antibodies against carp leukocytes, especially neutrophilic granulocytes and thrombocytes. Using their monoclonal antibodies, cell separation could be carried out with magnetic cell separation systems. Neutrophilic granulocytes separated showed non-specific cytotoxity as well as phagocvtosis. And this cytotoxic activity adapted to environmental temperatures. Aggregation capacity of carp thrombocytes was proved. These results provided some new informations out fish immune system clearly.
We carried out RAPD and ribosomal RNA gene analysIs on Leopeophtheirus salmonis, the major parasitic disease in UK aquaculture, which showed distinct populations from different areas around the Scotland coast.
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