1997 Fiscal Year Annual Research Report

変態、休眠を支配するホルモン分子の動態と環境応答

Research Project

Project/Area Number	08276101
Research Institution	The University of Tokyo
Principal Investigator	片岡宏誌東京大学, 大学院・農学生命科学研究科, 助教授 (60202008)
Co-Investigator(Kenkyū-buntansha)	溝口明名古屋大学, 大学院・理学研究科, 助教授 (60183109) 嶋田透東京大学, 大学院・農学生命科学研究科, 助教授 (20202111) 岩見雅史金沢大学, 理学部, 助教授 (40193768) 市川敏夫九州大学, 理学部, 助教授 (50136420) 相薗泰生神戸大学, 農学部, 教授 (20089931)
Keywords	カイコ / 休眠 / 変態 / ペプチドホルモン / エクジソン / 前胸腺刺激ホルモン / PBAN / ボンビキシン
Research Abstract	市川は、PBANを分泌する神経分泌細胞の活動を連続して記録することに成功し、日周リズム(昼高く、夜低い)を持つことを見い出した。また、岩見はボンビキシン遺伝子群の各遺伝子間のスペーサー領域(転写調節領域を含む)の全塩基配列を決定し、細胞特異的発現を調節していると思われるエレメントを推定した。相薗はPTTHの産生細胞がムスカリン性アセチルコリン受容体を発現していること、さらに脳-アラタ体器官培養系を用いて、アセチルコリンがPTTHA分泌を促進することを証明した。山下は環境温度による休眠ホルモン遺伝子の発現制御機構についてショウジョウバエへに休眠ホルモン遺伝子を導入し,解析を進めている。溝口はPTTH微量定量系を確立し、カイコ血液中のPTTH濃度の変動を詳細に解析し、PTTH濃度とそれに続くエクジステロイド濃度との間には相関が認められることなどを明らかにした。片岡は前胸腺に存在する分子量70kのPTTH受容体の同定を進め、その解離定数は54pMと極めて親和性が高いこと、5齢吐糸期の前胸腺には細胞1個あたり約16,000個存在していることを明らかにした。また、ボンビキシン受容体が中枢神経系や卵巣などに強く発現していることを示した。藤原は変態にともなうエクジソン受容体(EcR)遺伝子のアイソフォームの組織特異的な発現を解析し、2種類のアイソフォーム(A、B1)の発現パターンが成虫組織でAフォームが、幼虫組織でB1フォームが優勢であるという単純な発生運命には合致しないことを明らかにした。柳沼はカイコ卵から7種類のEcRアイソフォームをコードするcDNAをクローニングした。また、卵休眠の覚醒期に誘導されるソルビトール脱水素酵素遺伝子の5′-上流域にステロイドホルモン受容体ファミリーが認識結合する配列の存在を明らかにした。嶋田は休眠卵のみの初期胚に特異的に存在する、新規な2種類のmRNAを発現した。

Research Products

(34 results)

All Other

All Publications (34 results)

[Publications] Y.Aizono: "Prothoracicotropic hormone-producing neurosecretory cells in the silkworm,Bombyx mori." Brain Research. 763・1. 131-136 (1997)
[Publications] Y.Aizono: "Cholinergic and serotonergic control of prothoracicotropic hormone release during the pupal stage of the silkworm,Bombyx mori." Apple.Entomol.Zool.32・3. 508-511 (1997)
[Publications] Y.Shirai: "Carbachol-induced protein phosphorylation in the brain-corpus allatum complex of the silkworm,Bombyx mori." Appl.Entomol.Zool.32・4. 573-581 (1997)
[Publications] Ichikawa: "Activity patterns of neurosecretory cells relasing pheromonotropic neuropeptides in the moth,Bombyx mori." Proc.Natl.Acad.Sci.U.S.A.in press (1998)
[Publications] I.Shimizu: "Neuroendcrine control of diapause hormone secretion in the silkworm,Bombyx mori." J.Insect Physiol.43・12. 1001-1009 (1997)
[Publications] Masataka Suzuki: "Absence of dosage compensation at the transcription level of a sexlinked gene in a female heterogametic insect,Bombyx mori." Heredity. (in press). (1998)
[Publications] Y.Oda: "Dynamics of hemolymph sorbitol-6-phosphate and its control by ec dysteroid in the larvae of the silkworm,Bombyx mori." Insect Biochem.Mol Biol.27・6. 461-468 (1997)
[Publications] S.Tsuzuki: "Structure and expression of bombyxin E1 gene,a novel family gene that encodes bombyxin-IV,an insect insulin-related neurosecretory peptide." Comp.Biochem.Physiol.117B・3. 409-416 (1997)
[Publications] I.Yoshida: "Bombyxin F1 gene:structure and expression of a new bombyxin family gene that forms a pair with bombyxin B10gene." Zool.Sci.14・4. 615-622 (1997)
[Publications] 岩見雅史: "ボンビキシン:昆虫インシュリン様神経ペプチドホルモン" 生物科学ニュース. 303. 13-16 (1997)
[Publications] 岩見雅史: "インスリンと同じ先祖を持つ昆虫ホルモン-ボンビキシン" 化学と生物. 36・1. 8-10 (1998)
[Publications] 岩見雅史: "ボンビキシン:昆虫の変態を誘導するインスリン様ホルモン" 生化学. 70・4(印刷中). (1998)
[Publications] S.Satake: "Bombyxin,an Insulin-Related Peptide of Insects,Reduces the Major Storage Carbohydrates in the Silkworm Bombyx mori." Comp.Biochem.Physiol.118B・2. 349-357 (1997)
[Publications] Fujiwara H.: "Developmental profiles of wing imaginal discs of flugellos(f1),a wingless mutant of the silkworm,Bombyx mori." Dev.Genes Evol.207. 12-18 (1997)
[Publications] Hojyo T.: "Reciprocal transplantation of wing discs between a wing deficient mutant(f1)and wild type of the silkworm,Bombyx mori." Dev.Growth Differ.39. 599-606 (1997)
[Publications] Kamimura M.: "Tissue-specific and stage-specific expression of two silkworm ecdysone receptor isoforms--cdysteroid-dependent transcription in cultured anterior silk glands." Eur.J.Biochem.248. 786-793 (1997)
[Publications] Takahashi: "A new family of site-specific retrotransposons,SART1,is inserted into telomeric repeats of the silkworm,Bombyx mori." Nucl.Acids Res.25. 1578-1584 (1997)
[Publications] 藤原晴彦: "昆虫ステロイドホルモンによる転写制御" 化学と生物. 36. 75-77 (1997)
[Publications] T.Kawano: "Molecular cloning of a new type cDNA for pheromene biosynthesis activating neuropeptide in the silkworm,Bombyx mori." Biosci.Biotech.Biochem.61. 1745-1747 (1997)
[Publications] A.Suzuki: "Bombyx prothoracicotropic hormone:chemistry and biology." ACS Symposium Series. 658. 268-276 (1997)
[Publications] K.Nagata: "Three-dimensional structure and receptor-recognition sites of bombyxin-II,an insulin-like brain-secretory peptide of the silkmoth." Peptide Chemistry. (in press).
[Publications] S.Nagata: "Structure of glycoside of Bombyx mori prothoracicotropic hormone." Peptide Chemistry. (in press).
[Publications] S.-D.Ha: "Structure determination and cDNA cloning of paralytic peptide in Bombyx mori." Peptide Chemistry. (in press).
[Publications] Iwasaki,H.: "Cloning of cDNAs encoding Bombyx homologues of Cdc2 and Cdc2-related kinase from eggs." Insect Molecular Biology. 6・2. 131-141 (1997)
[Publications] Starnecker,G.,Koch: "Occurrence of four neuropeptides in the anterior central nervous system of the butterfly" Naturwissenschaften. 84. 152-154 (1997)
[Publications] Ikeda,M.: "Structure of the gene encoding vitellin-degrading protease of the silkworm,Bombyx mori." J.seric.Sci.Jpn.66・5. 346-350 (1997)
[Publications] Su,Z.-H.: "Structure of trehalase gene of the silkworm,Bombyx mori and phylogenic relationship of trehalases." J.Seric.Sci.Jpn.9・99. 457-465 (1997)
[Publications] Maki,N.: "Purification and characterization of a protease degrading 30kDa yolk proteins of the silkworm,Bombyx mori." Insect Biochem.Molec.Biol.27. 721-728 (1997)
[Publications] Shiomi,K.: "A hydrophobic peptide(VAP-peptide)of the silkworm,Bombyx mori:Structure,expression and an enhancing function of diapause hormone activity" Insect Biochem.Molec.Biol.(in press).
[Publications] Sato,Y.: "Phe-X-Pro-Arg-Leu-NH_2 peptide producing cells in the central nervous system of the silkworm,Bombyx mori." J.Insect Physiol.(in press).
[Publications] T.Niimi: "The profiles of mRNA levels for BHR39,a Bombyx homolog of Drosophila hormone receptor 39,and Bombyx FTZ-F1 in the course of embryonic development and diapause" Dev.Genes Evol.207・6. 410-412 (1997)
[Publications] K.Sato: "Baculovirus-mediated expression of a gene for trehalase of the mealworm beetle,Tenebrio molitor,in insect cells,SF-9,and larvae of the cabbage armyworm,Mamestra brassicae" Insect Biochem.Molec.Biol.(in press).
[Publications] 柳沼利信: "カイコの胚休眠とソルビトール脱水素酵素" 化学と生物. 35・7. 468-470 (1997)
[Publications] 藤原晴彦: "ホルモンの分子生物学8 昆虫ステロイドホルモンの受容と情報伝達" 学会出版センター(印刷中),

1997 Fiscal Year Annual Research Report

変態、休眠を支配するホルモン分子の動態と環境応答

Principal Investigator

片岡 宏誌 東京大学, 大学院・農学生命科学研究科, 助教授 (60202008)

Research Products

[Publications] Y.Aizono: "Prothoracicotropic hormone-producing neurosecretory cells in the silkworm,Bombyx mori." Brain Research. 763・1. 131-136 (1997)

[Publications] Y.Aizono: "Cholinergic and serotonergic control of prothoracicotropic hormone release during the pupal stage of the silkworm,Bombyx mori." Apple.Entomol.Zool.32・3. 508-511 (1997)

[Publications] Y.Shirai: "Carbachol-induced protein phosphorylation in the brain-corpus allatum complex of the silkworm,Bombyx mori." Appl.Entomol.Zool.32・4. 573-581 (1997)

[Publications] Ichikawa: "Activity patterns of neurosecretory cells relasing pheromonotropic neuropeptides in the moth,Bombyx mori." Proc.Natl.Acad.Sci.U.S.A.in press (1998)

[Publications] I.Shimizu: "Neuroendcrine control of diapause hormone secretion in the silkworm,Bombyx mori." J.Insect Physiol.43・12. 1001-1009 (1997)

[Publications] Masataka Suzuki: "Absence of dosage compensation at the transcription level of a sexlinked gene in a female heterogametic insect,Bombyx mori." Heredity. (in press). (1998)

[Publications] Y.Oda: "Dynamics of hemolymph sorbitol-6-phosphate and its control by ec dysteroid in the larvae of the silkworm,Bombyx mori." Insect Biochem.Mol Biol.27・6. 461-468 (1997)

[Publications] S.Tsuzuki: "Structure and expression of bombyxin E1 gene,a novel family gene that encodes bombyxin-IV,an insect insulin-related neurosecretory peptide." Comp.Biochem.Physiol.117B・3. 409-416 (1997)

[Publications] I.Yoshida: "Bombyxin F1 gene:structure and expression of a new bombyxin family gene that forms a pair with bombyxin B10gene." Zool.Sci.14・4. 615-622 (1997)

[Publications] 岩見雅史: "ボンビキシン:昆虫インシュリン様神経ペプチドホルモン" 生物科学ニュース. 303. 13-16 (1997)

[Publications] 岩見雅史: "インスリンと同じ先祖を持つ昆虫ホルモン-ボンビキシン" 化学と生物. 36・1. 8-10 (1998)

[Publications] 岩見雅史: "ボンビキシン:昆虫の変態を誘導するインスリン様ホルモン" 生化学. 70・4(印刷中). (1998)

[Publications] S.Satake: "Bombyxin,an Insulin-Related Peptide of Insects,Reduces the Major Storage Carbohydrates in the Silkworm Bombyx mori." Comp.Biochem.Physiol.118B・2. 349-357 (1997)

[Publications] Fujiwara H.: "Developmental profiles of wing imaginal discs of flugellos(f1),a wingless mutant of the silkworm,Bombyx mori." Dev.Genes Evol.207. 12-18 (1997)

[Publications] Hojyo T.: "Reciprocal transplantation of wing discs between a wing deficient mutant(f1)and wild type of the silkworm,Bombyx mori." Dev.Growth Differ.39. 599-606 (1997)

[Publications] Kamimura M.: "Tissue-specific and stage-specific expression of two silkworm ecdysone receptor isoforms--cdysteroid-dependent transcription in cultured anterior silk glands." Eur.J.Biochem.248. 786-793 (1997)

[Publications] Takahashi: "A new family of site-specific retrotransposons,SART1,is inserted into telomeric repeats of the silkworm,Bombyx mori." Nucl.Acids Res.25. 1578-1584 (1997)

[Publications] 藤原晴彦: "昆虫ステロイドホルモンによる転写制御" 化学と生物. 36. 75-77 (1997)

[Publications] T.Kawano: "Molecular cloning of a new type cDNA for pheromene biosynthesis activating neuropeptide in the silkworm,Bombyx mori." Biosci.Biotech.Biochem.61. 1745-1747 (1997)

[Publications] A.Suzuki: "Bombyx prothoracicotropic hormone:chemistry and biology." ACS Symposium Series. 658. 268-276 (1997)

[Publications] K.Nagata: "Three-dimensional structure and receptor-recognition sites of bombyxin-II,an insulin-like brain-secretory peptide of the silkmoth." Peptide Chemistry. (in press).

[Publications] S.Nagata: "Structure of glycoside of Bombyx mori prothoracicotropic hormone." Peptide Chemistry. (in press).

[Publications] S.-D.Ha: "Structure determination and cDNA cloning of paralytic peptide in Bombyx mori." Peptide Chemistry. (in press).

[Publications] Iwasaki,H.: "Cloning of cDNAs encoding Bombyx homologues of Cdc2 and Cdc2-related kinase from eggs." Insect Molecular Biology. 6・2. 131-141 (1997)

[Publications] Starnecker,G.,Koch: "Occurrence of four neuropeptides in the anterior central nervous system of the butterfly" Naturwissenschaften. 84. 152-154 (1997)

[Publications] Ikeda,M.: "Structure of the gene encoding vitellin-degrading protease of the silkworm,Bombyx mori." J.seric.Sci.Jpn.66・5. 346-350 (1997)

[Publications] Su,Z.-H.: "Structure of trehalase gene of the silkworm,Bombyx mori and phylogenic relationship of trehalases." J.Seric.Sci.Jpn.9・99. 457-465 (1997)

[Publications] Maki,N.: "Purification and characterization of a protease degrading 30kDa yolk proteins of the silkworm,Bombyx mori." Insect Biochem.Molec.Biol.27. 721-728 (1997)

[Publications] Shiomi,K.: "A hydrophobic peptide(VAP-peptide)of the silkworm,Bombyx mori:Structure,expression and an enhancing function of diapause hormone activity" Insect Biochem.Molec.Biol.(in press).

[Publications] Sato,Y.: "Phe-X-Pro-Arg-Leu-NH_2 peptide producing cells in the central nervous system of the silkworm,Bombyx mori." J.Insect Physiol.(in press).

[Publications] T.Niimi: "The profiles of mRNA levels for BHR39,a Bombyx homolog of Drosophila hormone receptor 39,and Bombyx FTZ-F1 in the course of embryonic development and diapause" Dev.Genes Evol.207・6. 410-412 (1997)

[Publications] K.Sato: "Baculovirus-mediated expression of a gene for trehalase of the mealworm beetle,Tenebrio molitor,in insect cells,SF-9,and larvae of the cabbage armyworm,Mamestra brassicae" Insect Biochem.Molec.Biol.(in press).

[Publications] 柳沼利信: "カイコの胚休眠とソルビトール脱水素酵素" 化学と生物. 35・7. 468-470 (1997)

[Publications] 藤原晴彦: "ホルモンの分子生物学8 昆虫ステロイドホルモンの受容と情報伝達" 学会出版センター(印刷中),

片岡宏誌東京大学, 大学院・農学生命科学研究科, 助教授 (60202008)