1999 Fiscal Year Annual Research Report

変態・休眠を支配するホルモン分子の動態と環境応答

Research Project

Project/Area Number	08276101
Research Institution	The University of Tokyo
Principal Investigator	片岡宏誌東京大学, 大学院・新領域創成科学研究科, 教授 (60202008)
Co-Investigator(Kenkyū-buntansha)	岩見雅史金沢大学, 大学院・自然科学研究科, 助教授 (40193768) 市川敏夫九州大学, 理学部, 助教授 (50136420) 相薗泰生神戸大学, 農学部, 教授 (20089931) 溝口明名古屋大学, 大学院・理学研究科, 助教授 (60183109) 嶋田透東京大学, 大学院・農学生命科学研究科, 助教授 (20202111)
Keywords	昆虫 / カイコ / ホルモン / エクジソン / 変態 / 休眠 / 遺伝子発現
Research Abstract	岩見はボンビキシンF1遺伝子のボンビキシン細胞特異的転写エレメントが、翻訳開始点より上流156〜169bpの領域に位置することを示した。市川はボンビキシン分泌細胞が、蛹期全期を通じて約30分周期の発火リズムがあることを見いだした。また、period遺伝子発現細胞は蛹中期以後、明期に活動が高く暗期に活動が低くなる概日リズムがあることを明らかにした。山下は形質転換ショウジョウバエを用いてDH-PBAN遺伝子の発現調節部位の同定を進め、-4233bから-4883にいたる650bpが最小限必要なシスエレメントであることを決定した。嶋田はカイコのperがZ染色体に座乗することや、第10染色体上のEcR・E75両遺伝子の座位を決定した。また、カイコで休眠卵特異的に発現する遺伝子BmEtsが、既知の休眠変異とは異なる遺伝子座であることを見いだした。相薗はエビガラスズメの休眠蛹の休眠覚醒がPTTH-エクジソンカスケードの起動により誘起されること、及びセロトニンによるPTTH分泌制御が休眠覚醒にとって重要であることを明らかにした。溝口はタバコスズメガPTTHに対する多種類のマウスモノクローナル抗体とウサギ抗血清を作製し、測定下限が0.1pgの超高感度測定法を開発した。片岡はカイコPTTH受容体のクローニングに成功し、膜7回貫通型受容体であることを明らかにした。藤原はカイコ蛹期の翅では、エクジソンが境界翅溝の外側で細胞死を、内側では細胞増殖を誘導し、メスの翅がないアカモンドクガでは、エクジソンがメス蛹期の翅全体での細胞死を誘導することを明らかにした。柳沼は中腸および卵巣におけるEcR-A、B1のタンパク量は、いずれの組織においても、5令幼虫初期では、Aタイプが豊富であり、吐糸期から蛹期のエクダイソンタイターの増加に伴い、Aタイプは減少し、対照的にB1タイプが増加していることを明らかにした。

Research Products
(28 results)

All Other

All Publications (28 results)

[Publications] M. G. Suzuki: "Bm Kettin, a hojmologue of the Drosophila kettin gene, is located on the Z chromosome in Bombyx mori and is not dosage compensated"Heredity. 82・2. 170-179 (1999)
[Publications] M. G. Suzuki: "Diapause-associated transcription of BmEts, a gene encoding an ETS transcription factor homolog in Bombyx mori"Insect Biochemistry and Molecular Biology. 29.4. 339-347 (1999)
[Publications] M. G. Suzuki: "The unfluence of triploidy on gene expression in the silkworm, Bombyx mori"Heredity. 82・6. 661-667 (1999)
[Publications] T. Yoshiga: "cDNA cloning of acyl-CoA desaturase homologs in the silkworm, Bombyx mori"Gene. (印刷中). (2000)
[Publications] Y. Oda: "Role of ecdysteroids in the dynamiccs of insect haemolymph sugar"Zool. Sci.. (in press). (2000)
[Publications] C. Fujii: "Essential tyrosine residues in 3-ketosteroid-delta 1-dehydrogenase from Rhodococcus rhodochrous"J. Biochem.. 126・4. 662-667 (1999)
[Publications] S. Morii: "Steroid monooxygenase of Rodococcus rhodochrous : sequencing of the genomic DNA and hyperexpression, purification, and charactrerization of the recombinant enzyme"J. Biochem. 126・3. 624-631 (1999)
[Publications] K. Moto: "Gene transfer into insect brain and cell-specific expression of bombyxin gene"Dev. Genes Evol.. 209・7. 447-450 (1999)
[Publications] Kamimura, M: "Expression of ecdysone receptor isoforms and trehalase in the anterior silkgland of Bombyx mori during an extra laval molt and precocious pupation induced by 20-hydroxyecdysone administration"Arch. Insect Biochem. Physiol.. 41. 79-88 (1999)
[Publications] Takahashi, H: "Transcription analysis of the telemeric repeat-specific retrotransposons TRAS1 and SART1 of the silkworm Bombyx mori"Nucl. Acids Res.. 27. 2015-2021 (1999)
[Publications] Matsuoka, T: "Expression of ecdysteroid-regulated genes is reduced specifically in the wing discs of the wing-deficient mutant (fl) of Bombyx mori"Dev. Genes Evol.. (in press). (2000)
[Publications] Fujiwara, H: "Telomeric structure of chromosomal fragments in two different mosaic strains of the silkworm, Bombyx mori"Zool. Sci. (in press). (2000)
[Publications] 藤原晴彦: "昆虫の擬態と変態:細胞生物学から見た虫の装いと衣替え-第一話蚕の紋様の話"ミクロスコピア. 16. 254-258 (1999)
[Publications] 藤原晴彦: "昆虫の擬態と変態:細胞生物学から見た虫の装いと衣替え-第二話華麗なるアゲハの変身"ミクロスコピア. (印刷中). (2000)
[Publications] T. Ichikawa: "Synchronous firing patterns of a set of insect neurosecretory cells"Neurosci. Lett.. 264. 85-88 (1999)
[Publications] T. Ichikawa: "Calling behavior modulates heartbeat reversal rhtythm in the silkmoth, Bombyx mori"Zool. Sci.. 16. 203-209 (1999)
[Publications] S. Stake: "Bombyxin secretion in the adult silkmoth Bombyx mori : Sex-specificity and its correlation with metabolism"J. Insect Physiol.. 45. 939-945 (1999)
[Publications] S. G. Dedos: "Differences between recombinant PTTH and crude brain extracts in cAM=-mediated ecdysteroid secretion from the prothoracic glands of the silkworm, Bombyx mori"J. Insect Physiol.. 45. 415-422 (1999)
[Publications] S. D. Ha: "Isolation and structure determination of a paralytic peptide from the hemolymph of Bombyx mori"Peptides. 20. 561-568 (1999)
[Publications] Y.-J. Hua: "Identification of a prothoracicostatic peptide (PTSP) from the larval brain of the silkworm, Bombyx mori"J. Biol. Chem.. 274. 31169-31173 (1999)
[Publications] K. Nagata: "Prothoracicotropic Activity of SBRPs, the insulin-like peptides of the Saturniid silkworm Samia cynthis ricini"Biochem. Biophys. Res. Commun.. 266. 575-578 (1999)
[Publications] W. Xu: "Molecular characterization of the cDNA encoding disapause hormone and pheromone biosynthesis activating neuropeptide in Bombyx mandarina"J. Seric. Sci. Jpn.. 68・5. 373-379 (1999)
[Publications] Y. Ishida: "The stage-and cell-specific expression of the Bombyx mori diapausehormone-pheromone biosynthesis activating neuropeptide (BomDH-PBAN) gene in the transformed Drosophila"J. Seric. Sci. Jpn.. 68・5. 417-427 (1999)
[Publications] T. Yaginuma: "Oxygen cosumption in relation to sorbitol utilization at the termination of diapause in eggs of the silkworm Bombyx mori"J. Insect Physiol.. 45. 621-627 (1999)
[Publications] Y. Jiang: "Neuroendocrine cascade involved in molting and metamolephosis in the sweetpotato hornworm, Agrius convolvuli"J. Seric. Sci. Jpn.. 68・5. 387-395 (1999)
[Publications] T. Uno: "Molecular cloning of cDNA for a small GTP binding protein, BRno from the embryo of Bombyx mori and its characterization after expression and purification"Arch. Insect Bichem. Physiol.. (in press). (2000)
[Publications] M. Masumura: "Glucose stimulates the release of bombyxin, an insulin-related peptide of the silkworm, Bombyx mori"Gen. Comp. Endocr,. (in press). (2000)
[Publications] Y. Hayakawa: "Distribution of growth-blocking peptide in the insect central nervous system"Cell Tissue Res.. (in press). (2000)

1999 Fiscal Year Annual Research Report

変態・休眠を支配するホルモン分子の動態と環境応答

Principal Investigator

片岡 宏誌 東京大学, 大学院・新領域創成科学研究科, 教授 (60202008)

Research Products

[Publications] M. G. Suzuki: "Bm Kettin, a hojmologue of the Drosophila kettin gene, is located on the Z chromosome in Bombyx mori and is not dosage compensated"Heredity. 82・2. 170-179 (1999)

[Publications] M. G. Suzuki: "Diapause-associated transcription of BmEts, a gene encoding an ETS transcription factor homolog in Bombyx mori"Insect Biochemistry and Molecular Biology. 29.4. 339-347 (1999)

[Publications] M. G. Suzuki: "The unfluence of triploidy on gene expression in the silkworm, Bombyx mori"Heredity. 82・6. 661-667 (1999)

[Publications] T. Yoshiga: "cDNA cloning of acyl-CoA desaturase homologs in the silkworm, Bombyx mori"Gene. (印刷中). (2000)

[Publications] Y. Oda: "Role of ecdysteroids in the dynamiccs of insect haemolymph sugar"Zool. Sci.. (in press). (2000)

[Publications] C. Fujii: "Essential tyrosine residues in 3-ketosteroid-delta 1-dehydrogenase from Rhodococcus rhodochrous"J. Biochem.. 126・4. 662-667 (1999)

[Publications] S. Morii: "Steroid monooxygenase of Rodococcus rhodochrous : sequencing of the genomic DNA and hyperexpression, purification, and charactrerization of the recombinant enzyme"J. Biochem. 126・3. 624-631 (1999)

[Publications] K. Moto: "Gene transfer into insect brain and cell-specific expression of bombyxin gene"Dev. Genes Evol.. 209・7. 447-450 (1999)

[Publications] Kamimura, M: "Expression of ecdysone receptor isoforms and trehalase in the anterior silkgland of Bombyx mori during an extra laval molt and precocious pupation induced by 20-hydroxyecdysone administration"Arch. Insect Biochem. Physiol.. 41. 79-88 (1999)

[Publications] Takahashi, H: "Transcription analysis of the telemeric repeat-specific retrotransposons TRAS1 and SART1 of the silkworm Bombyx mori"Nucl. Acids Res.. 27. 2015-2021 (1999)

[Publications] Matsuoka, T: "Expression of ecdysteroid-regulated genes is reduced specifically in the wing discs of the wing-deficient mutant (fl) of Bombyx mori"Dev. Genes Evol.. (in press). (2000)

[Publications] Fujiwara, H: "Telomeric structure of chromosomal fragments in two different mosaic strains of the silkworm, Bombyx mori"Zool. Sci. (in press). (2000)

[Publications] 藤原晴彦: "昆虫の擬態と変態:細胞生物学から見た虫の装いと衣替え-第一話 蚕の紋様の話"ミクロスコピア. 16. 254-258 (1999)

[Publications] 藤原晴彦: "昆虫の擬態と変態:細胞生物学から見た虫の装いと衣替え-第二話 華麗なるアゲハの変身"ミクロスコピア. (印刷中). (2000)

[Publications] T. Ichikawa: "Synchronous firing patterns of a set of insect neurosecretory cells"Neurosci. Lett.. 264. 85-88 (1999)

[Publications] T. Ichikawa: "Calling behavior modulates heartbeat reversal rhtythm in the silkmoth, Bombyx mori"Zool. Sci.. 16. 203-209 (1999)

[Publications] S. Stake: "Bombyxin secretion in the adult silkmoth Bombyx mori : Sex-specificity and its correlation with metabolism"J. Insect Physiol.. 45. 939-945 (1999)

[Publications] S. G. Dedos: "Differences between recombinant PTTH and crude brain extracts in cAM=-mediated ecdysteroid secretion from the prothoracic glands of the silkworm, Bombyx mori"J. Insect Physiol.. 45. 415-422 (1999)

[Publications] S. D. Ha: "Isolation and structure determination of a paralytic peptide from the hemolymph of Bombyx mori"Peptides. 20. 561-568 (1999)

[Publications] Y.-J. Hua: "Identification of a prothoracicostatic peptide (PTSP) from the larval brain of the silkworm, Bombyx mori"J. Biol. Chem.. 274. 31169-31173 (1999)

[Publications] K. Nagata: "Prothoracicotropic Activity of SBRPs, the insulin-like peptides of the Saturniid silkworm Samia cynthis ricini"Biochem. Biophys. Res. Commun.. 266. 575-578 (1999)

[Publications] W. Xu: "Molecular characterization of the cDNA encoding disapause hormone and pheromone biosynthesis activating neuropeptide in Bombyx mandarina"J. Seric. Sci. Jpn.. 68・5. 373-379 (1999)

[Publications] Y. Ishida: "The stage-and cell-specific expression of the Bombyx mori diapausehormone-pheromone biosynthesis activating neuropeptide (BomDH-PBAN) gene in the transformed Drosophila"J. Seric. Sci. Jpn.. 68・5. 417-427 (1999)

[Publications] T. Yaginuma: "Oxygen cosumption in relation to sorbitol utilization at the termination of diapause in eggs of the silkworm Bombyx mori"J. Insect Physiol.. 45. 621-627 (1999)

[Publications] Y. Jiang: "Neuroendocrine cascade involved in molting and metamolephosis in the sweetpotato hornworm, Agrius convolvuli"J. Seric. Sci. Jpn.. 68・5. 387-395 (1999)

[Publications] T. Uno: "Molecular cloning of cDNA for a small GTP binding protein, BRno from the embryo of Bombyx mori and its characterization after expression and purification"Arch. Insect Bichem. Physiol.. (in press). (2000)

[Publications] M. Masumura: "Glucose stimulates the release of bombyxin, an insulin-related peptide of the silkworm, Bombyx mori"Gen. Comp. Endocr,. (in press). (2000)

[Publications] Y. Hayakawa: "Distribution of growth-blocking peptide in the insect central nervous system"Cell Tissue Res.. (in press). (2000)

片岡宏誌東京大学, 大学院・新領域創成科学研究科, 教授 (60202008)

[Publications] 藤原晴彦: "昆虫の擬態と変態:細胞生物学から見た虫の装いと衣替え-第一話蚕の紋様の話"ミクロスコピア. 16. 254-258 (1999)

[Publications] 藤原晴彦: "昆虫の擬態と変態:細胞生物学から見た虫の装いと衣替え-第二話華麗なるアゲハの変身"ミクロスコピア. (印刷中). (2000)