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2000 Fiscal Year Final Research Report Summary

Dynamics and enviromental response of insect hormones controling metamorphosis and diapause

Research Project

Project/Area Number 08276101
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Research InstitutionThe University of Tokyo

Principal Investigator

KATAOKA Hiroshi  University of Tokyo, Department of Integrated Biosciences, Professor, 大学院・新領域創成科学研究科, 教授 (60202008)

Co-Investigator(Kenkyū-buntansha) IWAMI Masafumi  Kanazawa University, Department of Biology, Associate Professor, 大学院・自然科学研究科, 助教授 (40193768)
ICHIKAWA Toshio  Kyushu University, Department of Biology, Associate Professor, 理学部, 助教授 (50136420)
AIZONO Yasuo  Kobe University, Department of Agricultural Chemistry, Professor, 農学部, 教授 (20089931)
MIZOGUCHI Akira  Nagoya University, Department of Biology, Associate Professor, 大学院・理学研究科, 助教授 (60183109)
SHIMADA Toru  University of Tokyo, Department of Agricultural Biology, Associate Professor, 大学院・農学生命科学研究科, 助教授 (20202111)
Project Period (FY) 1996 – 1999
Keywordsinsect / silkworm / hormone / ecdysone / metamorphosis / diapause / cis-element
Research Abstract

Iwami et al. found that bombyxin gene is composed of 30 copies of related genes and identified a specific cis element for bombyxin-producing cells. Ichikawa et al. succeeded in monitoring electrical excitation of neurosecretory cells for 2-5 days. The electrical excitation of these cells producing the same neuropeptide was synchronized and regulated by other central neurons. Yamashita et al. identified a cis element composed of 650 bp for diapause hormone gene by using transgenic fruit fly, Drosophila. Shimada et al. identified gene loci of period, EcR and E75 genes. Aizono et al. found that prothoraicotropic hormone (PTTH) release regulated by serotonin is very important for arousal of sweet potato hornworm from pupal daiapause. Mizoguchi et al. developed a method to quantify a minute amount of PTTH and measured developmental changes of PTTH titer in hemolymph of Bombyx mori. Kataoka et al. identified a candidate of PTTH receptor cDNA by using expression cloning method. The receptor is belong to a family of G-protein coupled receptor. Fujiwara et al. found that expression levels of early late gene, BHR and late gene, urbain were very low in the wingless mutant of Bombyx mori (fl). Yaginuma et al. identified 7 isoforms of EcR.

  • Research Products

    (37 results)

All Other

All Publications (37 results)

  • [Publications] M.Iwami: "Bombyxin : an insect brain peptide that belongs to the insulin family."Zoological Science. 17. 1035-1044 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Iwami et al.: "Role of ecdysteroids in the dynamics of insect haemolymph sugar."Zoological Science. 17. 785-789 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Iwami et al.: "Ecdysteroid-inducible genes in the programmed cell death during insect metamorphosis."Insect Biochemistry and Molecular Biology. 31. 321-331 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Shimada et al.: "cDNA cloning of acyl-CoA desaturase homologs in the silkworm, Bombyx mori."Gene. 246. 339-345 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Shimada et al.: "Molecular structure of a novel gypsy-Ty3-like retrotranspon, Kabuki and nested retrotransposable elements on the W chromosome of the silkworm, Bombyx mori."Molecular and General Genetics. 263(6). 916-925 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Shimada et al.: "Identification and characterization of a silkworm ABC transporter gene homologous to Drosophila white."Molecular and General Genetics. 264(1). 11-20 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Mizoguchi et al.: "Carbohydrate metabolism during starvation in the silkworm Bombyx mori"Archives of insect biochemistry and Physiology. 44. 90-98 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Mizoguchi et al.: "Glucose stimulates the release of bombyxin, an insulin-related peptide of the silkworm, Bombyx mori."General and Comparative Endocrinology. 118. 393-399 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Mizoguchi et al.: "Developmental profile of the changes in the prothoracicotropic hormone titer in hemolymph of the silkworm Bombyx mori : correlation with ecdysteroid secretion."Insect Biochemistry and Molecular Biology. 31. 349-358 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Mizoguchi et al.: "Dynamic regulation of prothoracic gland ecdysteroidogenesis : Manduca sexta recombinant prothoracicotropic hormone and brain extracts haveidentical effects."Insect Biochemistry and Molecular Biology. 30(11). 1079-1091 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Mizoguchi et al.: "Distribution of growth-blocking peptide in the insect central nervous system."Cell and Tissue Research. 300(3). 459-464 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Fujiwara et al.: "Expression of ecdysteroid-regulated genes is reduced specifically in the wing discs of the wing-deficient mutant (fl) of Bombyx mori. Development"Genes and Evolution. 210. 120-128 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Fujiwara et al.: "Detection and distribution patterns of telomerase activity in insects."European Journal of Biochemistry. 267. 1-8 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Fujiwara et al.: "Stability and telomere structure of chromosomal fragments in two different mosaic strains of the silkworm, Bombyx mori."Zoological Science. 17. 743-750 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] O.Yamashita et al.: "Structure of the VAP-peptide (BmACP-6.7) gene in the silkworm, Bombyx mori and a possible regulation of its expression by BmFTZ-F1."Insect Biochemistry and Molecular Biology. 30. 119-125 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] O.Yamashita et al.: "The cis-regulatory region responsible for the BomDH-PBAN gene expression in FXPRLamide peptide producing neurosecretory cells of the transformed Drosophila."The Journal of Sericultural Science of Japan. 69(2). 111-119 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Kataoka et al.: "Cloning and sequence analysis of cDNA for diuretic hormone receptor from the Bombyx mori."Mol.Cells. 10. 13-17 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Kataoka et al.: "Molecular characterization of a cDNA from the true armyworm pseudaletia unipuncta encoding manduca sexta allatotropin peptide."Insect Biochem.Mol.Biol.. 30. 691-702 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 市川敏夫: "昆虫の変態と脳の再編成.もうひとつの脳-微小脳の研究入門(富永・宗岡 編)"倍風館. (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Iwami et al.: "Bombyxin : an insect brain peptide that belongs to the insulin family."Zoological Science. 17. 1035-1044 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Iwami et al.: "Role of ecdysteroids in the dynamics of insect haemolymph sugar."Zoological Science. 17. 785-789 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Iwami et al.: "Genes induced by ecdysteroid in the programed cell death of the anterior silk gland."Insect Biochemistry and Molecular Biology. 31. 321-331 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Shimada et al.: "cDNA cloning of acyl-CoA desaturase homologs in the silkworm, Bombyx mori."Gene. 246. 339-345

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Shimada et al: "Molecular structure of a novel gypsy-Ty3-like retrotranspon, Kabuki and nested retrotransposable elements on the W chromosome of the silkworm, Bombyx mori."Molecular and General Genetics. 263. 916-952 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Shimada et al.: "Identification and characterization of a silkworm ABC transporter gene homologous to Drosophila white."Molecular and General Genetics. 264. 11-20 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A.Mizoguchi et al.: "Carbohydrate metabolism during starvation in the silkworm Bombyx mori"Archives of insect biochemistry and Physiology. 44. 90-98 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A.Mizoguchi et al.: "Glucose stimulates the release of bombyxin, an insulin-related peptide of the silkworm, Bombyx mori."General and Comparative Endocrinology. 118. 393-399 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A.Mizoguchi et al.: "Developmental profile of the changes in the prothoracicotropic hormone titer in hemolymph of the silkworm Bombyx mori : correlation with ecdysteroid secretion."Insect Biochemistry and Molecular Biology. 31. 349-358 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A.Mizoguchi et al.: "Dynamic regulation of prothoracic gland ecdysteroidogenesis : Manduca sexta recombinant prothoracicotropic hormone and brain extracts haveidentical effects."Insect Biochemistry and Molecular Biology. 30. 1079-1091 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A.Mizoguchi et al.: "Distribution of growth-blocking peptide in the insect central nervous system."Cell and Tissue Research. 300. 459-462 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Fujiwara et al.: "Expression of ecdysteroid-regulated genes is reduced specifically in the wing discs of the wing-deficient mutant (fl) of Bombyx mori."Development, Genes and Evolution. 210. 120-128 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Fujiwara et al.: "Detection and distribution patterns of telomerase activity in insects."European Journal of Biochemistry. 267. 1-8 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Fujiwaraet al.: "Stability and telomere structure of chromosomal fragments in two different mosaic strains of the silkworm, Bombyx mori."Zoological Science. 17. 743-750 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] O.Yamashita et al.: "Structure of the VAP-peptide (BmACP-6.7) gene in the silkworm, Bombyx mori and a possible regulation of its expression by BmFTZ-F1."Insect Biochemistry and Molecular Biology. 30. 119-125 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] O.Yamashita et al.: "The cis-regulatory region responsible for the BomDH-PBAN gene expression in FXPRLamide peptide producing neurosecretory cells of the transformed Drosophila."The Journal of Sericultural Science of Japan. 69(2). 111-119 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Kataoka et al.: "Cloning and sequence analysis of cDNA for diuretic hormone receptor from the Bombyx mori."Mol.Cells.. 10. 13-17 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Kataoka et al.: "Molecular characterization of a cDNA from the true armyworm pseudaletia unipuncta encoding manduca sexta allatotropin peptide."Insect Biochem.Mol.Biol.. 30. 691-702 (2000)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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