1997 Fiscal Year Annual Research Report

植物細胞における空胞系(Vacuolar System)の動態の分子機構

Research Project

Project/Area Number	08304044
Research Institution	National Institute for Basic Biology
Principal Investigator	大隅良典岡崎国立共同研究機構, 基礎生物学研究所, 教授 (30114416)
Co-Investigator(Kenkyū-buntansha)	前島正義名古屋大学, 農学部, 助教授 (80181577) 森山芳則大阪大学, 産業科学研究所, 助教授 (10150658) 森安裕二静岡県立大学, 食品栄養科学部, 助手 (20200454) 中村研三名古屋大学, 農学部, 教授 (80164292) 竹川薫香川大学, 農学部, 助教授 (50197282)
Keywords	小胞体 / ゴルジ体 / 液胞 / 水チャネル / 貯蔵タンパク質 / 自食作用 / 小胞輸送 / リン酸輸送
Research Abstract	真核細胞は膜系を発達させ、コンパートメントを形成することを一つの大きな特徴とする。この細胞内コンパートメントはそれぞれ特徴的かつ特殊化された環境をもち、細胞内での機能的、空間的な機能分化をおこなっている。この各オルガネラは独自の機能を保ちながら、膜小胞を介して相互に物質を交換し、その相互の関係はきわめて動的なものであること明きらかにされてきた。植物細胞における空胞系の機能は植物の生理機能に重要であることは、以前から注目されており、その動態を分子レベルで解析することは植物細胞の理解に不可欠の課題である。本研究では、酵母、植物、動物細胞を対象に研究を行っている研究者を11名の班員をもって構成され、各々の研究のもつ背景とこれまでの成果を相互に比較、分析し、さらに新たな研究手法、概念の確立を計り、植物細胞における空胞系の動態の分子メカニズムを明らかにすることを目的とした。今年度もそれぞれの系で多くの成果を得ることが出来た。大隅と森安は自食作用に関する新しい知見を得た。野口と中野はER、ゴルジ間の輸送と局在化機構に関する新しい知見を得た。和田、西村は液胞形成、機能分化の分子レベルでの解析を進めた。中村、竹川は液胞酵素のターゲティング機構を明らかにした。前島は水チャネルの動態と機能解析を行った。三村はリン酸輸送の制御機構を解析した。森山はミクロベシクルの解析から細胞内情報伝達機構を解明した。本研究領域は世界的にも日進月歩のであり、今後の更なる発展により、植物細胞の本質的な理解と、動物細胞にはない独自の戦略の理解につなが留ことが期待される。

Research Products
(43 results)

All Other

All Publications (43 results)

[Publications] Baba,M.他4名: "Two distinct pathways for targeting proteins from the cytoplasm to the vacuole/lysosome." J.Cell Biol.139. 1687-1695 (1997)
[Publications] Beilby,M.他2名: "Perfusion of charophyte cells : A critical analysis of the method." Journal of Experimental Botany. 48. 157-172 (1997)
[Publications] Fleurat-Lessard 他5名: "Increased expression of vacuolar aquaporin and H^+-ATPase related to motor cell function in Mimosa pudica L." Plant Physiol.114. 827-834 (1997)
[Publications] Funakoshi,T.他3名: "Analyses of APG13 gene involved in the autophagy in yeast,Saccharomyces cerevisiae." Gene. 192. 207-213 (1997)
[Publications] Hatano K.他4名: "A rapid increase in the level of binding protein (BiP) is accompanied by synthesis and degradation of storage proteins in pumpkin cotyledons." Plant Cell Physiol.38. 344-351 (1997)
[Publications] Hiraiwa,N.他2名: "Expression and activation of the vacuolar processing enzyme in Saccharomyces cerevisiae.." Plant J.12. 819-829 (1997)
[Publications] Hiraiwa,N.他2名: "An aspartic proteinase is involved in breakdown of propeptides of storage proteins in protein-storage vacuoles of plants." Eur.J.Biochem.246. 133-141 (1997)
[Publications] Inoue,K.他3名: "Heterologous expression and subcellular localization of pumpkin seed tonoplast intrinsic proteins (TIP) in yeast cells." Plant Cell Physiol.38. 366-370 (1997)
[Publications] Kanda,H.他5名: "Identification of a vanadium-associated protein from the vanadium-rich ascidian,Ascidia sydneiensis samea." Zool.Sci.14. 37-42 (1997)
[Publications] Kawauchi,K., 他9名: "A possible immunosuppressant,cycloprodigiosin hydrochloride,obtained from Pseudoalteromonas denitrificans." Biochem.Biophys.Res.Commun.237. 543-547 (1997)
[Publications] Koide,Y.他3名: "The N-terminal propeptide of the precursor to sporamin acts as a vacuolar targeting signal even at the C-terminus of the mature part in tobacco cells." Plant Physiol.114. 863-870 (1997)
[Publications] Matsuoka,K.他3名: "A vacuolar-type H^+-ATPase in non-vacuolar organelle is required for the sorting of soluble vacuolar protein precursors in tobacco cells." Plant Cell. 9. 533-546 (1997)
[Publications] Matsuura,A.他2名: "Apg1p,a novel protein kinase required for the autophagic process in Saccharomyces cerevisiae." Gene. 192. 245-250 (1997)
[Publications] Mimura,T.他5名: "Efficient callus initiation from leaf of mangrove plant,Bruguiera sexangula in amino acid medium : Effect of NaCl on callus initiation." Journal of Plant Research. 110. 25-29 (1997)
[Publications] Mimura,T.他3名: "NaCl-dependent growth,ion content and regeneration of calluses initiated from the mangrove plant,Bruguiera sexangula." Journal of Plant Research. 110. 31-36 (1997)
[Publications] Minami,Y.他7名: "β-Glucosidase in the Indigo plant : intracellular localization and tissue specific expression in leaves." Plant Cell Physiol.38. 1069-1074 (1997)
[Publications] Nakamura,I.他7名: "Failure of bone resorption in osteosclerotic (oc/oc) mice is possibly due to the dissociation of vacuolar proton ATPase and cytoskelton in osteoclasts." FEBS Letters. 401. 207-212 (1997)
[Publications] Nakamura,N.他3名: "Vam2/Vps41p and Vam6/Vps39p are components of a protein complex on the vacuolar membrane and involved in the vacuolar assembly in the yeast Saccharomyces cerevisiae." J.Biol.Chem.272. 11344-11349 (1997)
[Publications] Nakamura,N.他3名: "Acidification inside the vacuole is required for degradation of autopahagosomes in the yeast Saccharomyces cerevisiae." J.Biochem.121. 338-344 (1997)
[Publications] Nose,Y.他5名: "Specific increase in the number of vanadium-containing blood cells by some ionophores-ATPase in the ascidian Ascidia sydneiensis samea." Zool.Sci.14. (1997)
[Publications] Sato,K.& Nakano,A.: "Rer1p as common machinery for the endoplasmic reticulum localization of membrane proteins." Proc.Natl.Acad.Sci.U.S.A.94. 9693-9698 (1997)
[Publications] Sato,M.H.and Wada,Y.: "Universal template plasmid for introduction of the triple HA-epitope sequence into the cloned genes." Bio Techniques. 23. 254-256 (1997)
[Publications] Sato,M.H.他7名: "The AtVAM3 encodes a syntaxin related molecule implicated in the vacuolar assembly in Arabidopsis thaliana." J.Biol.Chem.272. 24530-24535 (1997)
[Publications] Sato,Y.他2名: "A tobacco cDNA encoding a homologue of N-ethylmaleimide sensitive fusion protein (NSF) from tobacco." Plant Physiol.113. 1464 (1997)
[Publications] Scott,S.V.他3名: "Aminopeptidase I is targeted to the vacuole by a nonclassical vescicular mechanism." J.Cell Biol.138. 37-44 (1997)
[Publications] Shimada,T.他3名: "A pumpkin 72-kDa membrane protein of precursor-accumulating vesicles has characteristics of a vacuolar sorting receptor." Plant Cell Physiol.38. 1414-1420 (1997)
[Publications] Shirahama,K.他2名: "Mutational analysis of Csc1/Vps2p-involvement of endosome in regulation of the autophagy in yeast" Cell Struc.Func.22. 501-509 (1997)
[Publications] Shiratake,K.他3名: "Changes in H^+-pumps and a tonoplast intrinsic protein of vacuolar membranes during the development of pear fruit." Plant Cell Physiol.38. 1039-1045 (1997)
[Publications] Tabuchi,M.他7名: "Vacuolar protein sorting in fission yeast : cloning,biosynthesis,transport,and processing of carboxypeptidase Y from Schizosaccharomyces pombe." J.Bacteriol.179. 4179-4189 (1997)
[Publications] Tabuchi,M.他3名: "The Schizosaccharomyces pombe gms1^+ gene encodes an UDP-galactose transporter homologue required for protein galactosylation." Biochem.Biophys.Res.Commun.232. 121-125 (1997)
[Publications] Takasu,A.他3名: "Analysis of substrate binding site and carboxyl terminal region of vacuolar H^+ translocating pyrophosphatase of mung bean with peptide antibodies." J.Biochem.122. 883-889 (1997)
[Publications] Tomochika,K.他5名: "Vacuolar ATPase in mouse bladder epithelium is responsible for urinary acidification." FEBS Letters. 404. 61-64 (1997)
[Publications] Ueda,K.and Noguchi,T.: "The number and the distribution pattern of Golgi bodies in cells of Micrasterias (Conjugales,Chlorophyta) examined by fluorescence microscopy and by electron microscopy." Phycological Research.45. 133-139 (1997)
[Publications] Uyama,T.他4名: "Finding of the same antigens in the Polychaete,Pseudopotamilla occelate,as those in the vanadium-rich ascidian,Ascidia sydneiensis samea." Zool.Sci.14. 43-47 (1997)
[Publications] Wada,Y.他3名: "Vam3p,a new member of syntaxin related protein,is required for vacuolar assembly in the yeast Saccharomyces cerevisiae." J.Cell.Sci.110. 1299-1306 (1997)
[Publications] Yamada,H.他2名: "L-Aspartate-evoked inhibition of melatonin production in rat pineal glands." Neurosci.Lett.228. 103-106 (1997)
[Publications] Yamada,H.他7名: "Functional expression of a GLT-1 type Na^+-dependent glutamate transporter in rat pinealocytes." J.Neurochem.69. 1491-1498 (1997)
[Publications] Yatsushiro,他5名: "L-Aspartate but not the D-form is secreted through microvesicle-mediated exocytosis and is sequestered through Na^+-dependent transporter in rat pinealocytes." J.Neurochem.69. 340-347 (1997)
[Publications] Kato,A.他5名: "Glyoxysomal malate dehydrogenase in pumpkin : cloning of a cDNA and functional analysis of its prosequence." Plant Cell Physiol.39. 186-195 (1998)
[Publications] Mimura,T.他2名: "Control of phosphate transport across the plasma membrane of Chara corallina." Journal of Experimental Botany. 49. 13-19 (1998)
[Publications] Noda,T.& Ohsumi,Y.: "Tor,a phosphatidylinositol kinase homologue,controls autophagy in yeast." J.Biol.Chem.273. 3963-3966 (1998)
[Publications] Ueda,T.他5名: "AtGDI2,a novel Arabidopsis gene encoding a Rab GDP dissociation inhibitor." Gene. 206. 137-143 (1998)
[Publications] Yamamoto,A.他8名: "Bafilomycin A1 prevents maturation of autophagic vacuoles by inhibiting fusion betwee autophagosomes and lysosomes in rat hepatoma cell line,H-4-II-E cells." Cell Struct.Funct.23. 33-42 (1998)

1997 Fiscal Year Annual Research Report

植物細胞における空胞系(Vacuolar System)の動態の分子機構

Principal Investigator

大隅 良典 岡崎国立共同研究機構, 基礎生物学研究所, 教授 (30114416)

Research Products

[Publications] Baba,M.他4名: "Two distinct pathways for targeting proteins from the cytoplasm to the vacuole/lysosome." J.Cell Biol.139. 1687-1695 (1997)

[Publications] Beilby,M.他2名: "Perfusion of charophyte cells : A critical analysis of the method." Journal of Experimental Botany. 48. 157-172 (1997)

[Publications] Fleurat-Lessard 他5名: "Increased expression of vacuolar aquaporin and H^+-ATPase related to motor cell function in Mimosa pudica L." Plant Physiol.114. 827-834 (1997)

[Publications] Funakoshi,T.他3名: "Analyses of APG13 gene involved in the autophagy in yeast,Saccharomyces cerevisiae." Gene. 192. 207-213 (1997)

[Publications] Hatano K.他4名: "A rapid increase in the level of binding protein (BiP) is accompanied by synthesis and degradation of storage proteins in pumpkin cotyledons." Plant Cell Physiol.38. 344-351 (1997)

[Publications] Hiraiwa,N.他2名: "Expression and activation of the vacuolar processing enzyme in Saccharomyces cerevisiae.." Plant J.12. 819-829 (1997)

[Publications] Hiraiwa,N.他2名: "An aspartic proteinase is involved in breakdown of propeptides of storage proteins in protein-storage vacuoles of plants." Eur.J.Biochem.246. 133-141 (1997)

[Publications] Inoue,K.他3名: "Heterologous expression and subcellular localization of pumpkin seed tonoplast intrinsic proteins (TIP) in yeast cells." Plant Cell Physiol.38. 366-370 (1997)

[Publications] Kanda,H.他5名: "Identification of a vanadium-associated protein from the vanadium-rich ascidian,Ascidia sydneiensis samea." Zool.Sci.14. 37-42 (1997)

[Publications] Kawauchi,K., 他9名: "A possible immunosuppressant,cycloprodigiosin hydrochloride,obtained from Pseudoalteromonas denitrificans." Biochem.Biophys.Res.Commun.237. 543-547 (1997)

[Publications] Koide,Y.他3名: "The N-terminal propeptide of the precursor to sporamin acts as a vacuolar targeting signal even at the C-terminus of the mature part in tobacco cells." Plant Physiol.114. 863-870 (1997)

[Publications] Matsuoka,K.他3名: "A vacuolar-type H^+-ATPase in non-vacuolar organelle is required for the sorting of soluble vacuolar protein precursors in tobacco cells." Plant Cell. 9. 533-546 (1997)

[Publications] Matsuura,A.他2名: "Apg1p,a novel protein kinase required for the autophagic process in Saccharomyces cerevisiae." Gene. 192. 245-250 (1997)

[Publications] Mimura,T.他5名: "Efficient callus initiation from leaf of mangrove plant,Bruguiera sexangula in amino acid medium : Effect of NaCl on callus initiation." Journal of Plant Research. 110. 25-29 (1997)

[Publications] Mimura,T.他3名: "NaCl-dependent growth,ion content and regeneration of calluses initiated from the mangrove plant,Bruguiera sexangula." Journal of Plant Research. 110. 31-36 (1997)

[Publications] Minami,Y.他7名: "β-Glucosidase in the Indigo plant : intracellular localization and tissue specific expression in leaves." Plant Cell Physiol.38. 1069-1074 (1997)

[Publications] Nakamura,I.他7名: "Failure of bone resorption in osteosclerotic (oc/oc) mice is possibly due to the dissociation of vacuolar proton ATPase and cytoskelton in osteoclasts." FEBS Letters. 401. 207-212 (1997)

[Publications] Nakamura,N.他3名: "Vam2/Vps41p and Vam6/Vps39p are components of a protein complex on the vacuolar membrane and involved in the vacuolar assembly in the yeast Saccharomyces cerevisiae." J.Biol.Chem.272. 11344-11349 (1997)

[Publications] Nakamura,N.他3名: "Acidification inside the vacuole is required for degradation of autopahagosomes in the yeast Saccharomyces cerevisiae." J.Biochem.121. 338-344 (1997)

[Publications] Nose,Y.他5名: "Specific increase in the number of vanadium-containing blood cells by some ionophores-ATPase in the ascidian Ascidia sydneiensis samea." Zool.Sci.14. (1997)

[Publications] Sato,K.& Nakano,A.: "Rer1p as common machinery for the endoplasmic reticulum localization of membrane proteins." Proc.Natl.Acad.Sci.U.S.A.94. 9693-9698 (1997)

[Publications] Sato,M.H.and Wada,Y.: "Universal template plasmid for introduction of the triple HA-epitope sequence into the cloned genes." Bio Techniques. 23. 254-256 (1997)

[Publications] Sato,M.H.他7名: "The AtVAM3 encodes a syntaxin related molecule implicated in the vacuolar assembly in Arabidopsis thaliana." J.Biol.Chem.272. 24530-24535 (1997)

[Publications] Sato,Y.他2名: "A tobacco cDNA encoding a homologue of N-ethylmaleimide sensitive fusion protein (NSF) from tobacco." Plant Physiol.113. 1464 (1997)

[Publications] Scott,S.V.他3名: "Aminopeptidase I is targeted to the vacuole by a nonclassical vescicular mechanism." J.Cell Biol.138. 37-44 (1997)

[Publications] Shimada,T.他3名: "A pumpkin 72-kDa membrane protein of precursor-accumulating vesicles has characteristics of a vacuolar sorting receptor." Plant Cell Physiol.38. 1414-1420 (1997)

[Publications] Shirahama,K.他2名: "Mutational analysis of Csc1/Vps2p-involvement of endosome in regulation of the autophagy in yeast" Cell Struc.Func.22. 501-509 (1997)

[Publications] Shiratake,K.他3名: "Changes in H^+-pumps and a tonoplast intrinsic protein of vacuolar membranes during the development of pear fruit." Plant Cell Physiol.38. 1039-1045 (1997)

[Publications] Tabuchi,M.他7名: "Vacuolar protein sorting in fission yeast : cloning,biosynthesis,transport,and processing of carboxypeptidase Y from Schizosaccharomyces pombe." J.Bacteriol.179. 4179-4189 (1997)

[Publications] Tabuchi,M.他3名: "The Schizosaccharomyces pombe gms1^+ gene encodes an UDP-galactose transporter homologue required for protein galactosylation." Biochem.Biophys.Res.Commun.232. 121-125 (1997)

[Publications] Takasu,A.他3名: "Analysis of substrate binding site and carboxyl terminal region of vacuolar H^+ translocating pyrophosphatase of mung bean with peptide antibodies." J.Biochem.122. 883-889 (1997)

[Publications] Tomochika,K.他5名: "Vacuolar ATPase in mouse bladder epithelium is responsible for urinary acidification." FEBS Letters. 404. 61-64 (1997)

[Publications] Ueda,K.and Noguchi,T.: "The number and the distribution pattern of Golgi bodies in cells of Micrasterias (Conjugales,Chlorophyta) examined by fluorescence microscopy and by electron microscopy." Phycological Research.45. 133-139 (1997)

[Publications] Uyama,T.他4名: "Finding of the same antigens in the Polychaete,Pseudopotamilla occelate,as those in the vanadium-rich ascidian,Ascidia sydneiensis samea." Zool.Sci.14. 43-47 (1997)

[Publications] Wada,Y.他3名: "Vam3p,a new member of syntaxin related protein,is required for vacuolar assembly in the yeast Saccharomyces cerevisiae." J.Cell.Sci.110. 1299-1306 (1997)

[Publications] Yamada,H.他2名: "L-Aspartate-evoked inhibition of melatonin production in rat pineal glands." Neurosci.Lett.228. 103-106 (1997)

[Publications] Yamada,H.他7名: "Functional expression of a GLT-1 type Na^+-dependent glutamate transporter in rat pinealocytes." J.Neurochem.69. 1491-1498 (1997)

[Publications] Yatsushiro,他5名: "L-Aspartate but not the D-form is secreted through microvesicle-mediated exocytosis and is sequestered through Na^+-dependent transporter in rat pinealocytes." J.Neurochem.69. 340-347 (1997)

[Publications] Kato,A.他5名: "Glyoxysomal malate dehydrogenase in pumpkin : cloning of a cDNA and functional analysis of its prosequence." Plant Cell Physiol.39. 186-195 (1998)

[Publications] Mimura,T.他2名: "Control of phosphate transport across the plasma membrane of Chara corallina." Journal of Experimental Botany. 49. 13-19 (1998)

[Publications] Noda,T.& Ohsumi,Y.: "Tor,a phosphatidylinositol kinase homologue,controls autophagy in yeast." J.Biol.Chem.273. 3963-3966 (1998)

[Publications] Ueda,T.他5名: "AtGDI2,a novel Arabidopsis gene encoding a Rab GDP dissociation inhibitor." Gene. 206. 137-143 (1998)

[Publications] Yamamoto,A.他8名: "Bafilomycin A1 prevents maturation of autophagic vacuoles by inhibiting fusion betwee autophagosomes and lysosomes in rat hepatoma cell line,H-4-II-E cells." Cell Struct.Funct.23. 33-42 (1998)

大隅良典岡崎国立共同研究機構, 基礎生物学研究所, 教授 (30114416)