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1997 Fiscal Year Final Research Report Summary

Molecular Mechanism of Dynamics of Vacuolar System in Plant Cell

Research Project

Project/Area Number 08304044
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理
Research InstitutionNational Institute for Basic Biology

Principal Investigator

OHSUMI Yoshinori  National Institute for Basic Biology, Nat.Inst.Basic Biol., Prof., 基礎生物学研究所, 教授 (30114416)

Co-Investigator(Kenkyū-buntansha) MAESHIMA Masayoshi  National Institute for Basic Biology, Dept of Agr.Sci., Associate Prof., 農学部, 助教授 (80181577)
MORIYAMA Yoshinori  ISIR,Osaka Univ., Associate Prof., 産業科学研究所, 助教授 (10150658)
MORIYASU Yuji  National Institute for Basic Biology, Faclt.of Int.Rel., Assitant Prof., 食品栄養科学部, 助手 (20200454)
NAKAMURA Kenzo  National Institute for Basic Biology, Dept.Agr.Sci., Prof., 農学部, 教授 (80164292)
TAKEGAWA Kaoru  National Institute for Basic Biology, Faclt.of Agr., Associate Prof., 農学部, 助教授 (50198272)
Project Period (FY) 1996 – 1997
KeywordsER / Golgi Body / Vacuole / Storage Protein / Water Channel / Autophagy / Vesicular Transport / Phosphate Transport
Research Abstract

One of the most important features of eukaryotic cells is developement of complex intracellular single membrane system called vacuolar system. ER,Golgi body, lysosome and plasma membrane are dynamically connected each other by specific vesicles. In plant cell still not much is known about the molecular machinery for the dynamic aspect of the vesicular trafficing. This research program aimed to elucidate specific features of vacuolar system in plant cells, by comparing various model systems which each group is working with including yeast, Green algi, culture cell and higher plant cell. We could discuss about many different interesting results obtained by each group and several cooperation started during this research program.
On the mechanisms of bulk protein degradation (autophagy), protein trafficing for storage protein, vacuolar protein and membrane biogenesis, water channel, many important findings were reported.

  • Research Products

    (122 results)

All Other

All Publications (122 results)

  • [Publications] Baba, M.他4名: "Two distinct pathways for targeting proteins from the cytoplasm to the vacuole/lysosome." J.Cell Biol. 139. 1687-1695 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Beilby, M.他2名: "Perfusion of charophyte cells:A critical analysis of the method." Journal of Experimental Botany. 48. 157-172 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fleurat-Lessard 他5名: "Increased expression of vacuolar aquaporin and H^+-ATPase related to motor ce11 function in Mimosa pudica L." Plant Physiol.114. 827-834 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Funakoshi, T 他3名: "Analyses of APG13 gene involved in the autophagy in yeast,Saccharomyces cerevisiae." Gene. 192. 207-213 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hatano K.他4名: "A rapid increase in the level of binding protein(BiP)is accompanied by synthesis and degradation of storage proteins in pumpkin cotyledons." Plant Cell Physiol.38. 344-351 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiraiwa, N.他2名: "Expression and activation of the vacuolar processing enzyme in Saccharomyces cerevisiae." Plant J.12. 819-829 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiraiwa, N.他2名: "An aspartic proteinase is involved in breakdown of propeptides of storage proteins in protein-storage vacuoles of plants." Eur.J.Biochem.246. 133-141 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Inoue.K.他3名: "Heterologous expression and subcellar localization of pumpkin seed tonoplast intrinsic proteins(TIP)in yeast cells." Plant Cell Physiol.38. 366-370 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kanda, H.他5名: "Identification of a vanadium-associated protein from the vanadium -rich ascidian,Ascidia sydneiensis samea." Zool.Sci.14. 37-42 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawauchi, K., 他9名: "A possible immunosuppressant,cycloprodigiosin hydrochloride,obtained from pseudoalteromonas deritnificans" Biochem.Biophys.Res.Commun.237. 543-547 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Koide, Y.他3名: "The N-terminal propeptide of the precursor to sporamin acts as a vacuolar targeting signal even at the C-terminus of the mature part in tobacco cells." Plant Physiol.114. 863-870 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsuoka, K.他3名: "A vacuolar-type H^+-ATPase in non-vacuolar organelle is required for sorting of soluble vacuolar protein prceersors in tobacco cells." Plant cell. 9. 533-541 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsuura, A 他2名: "Apglp,a novel protein kinase required for the autophagic process In Saccharomyces cerevisiae." Gene. 192. 245-250 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mimura, T.他5名: "Efficient callus initiation from leaf of mangrove plant,Bruguiera sexangula in amino acid medium:Effect of NaCI on callus initiation." Journal of Plant Research. 110. 25-29 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mimura, T.他3名: "NaCl-dependent growth,ion content and regeneration of calluses initiated from the mangrove plant,Bruguiera sexangula." Journal of Plant Research. 110. 31-36 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Minami, Y.他7名: "β-Glucosidase in the Indigo plant:intracellular localization and tissue specific expression in leaves." Plant Cell Physiol.38. 1069-1074 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakamura, I.他7名: "Failure of bone resorption in osteosclerotic(oc/oc)mice is possibly due to the dissociation of vacuolar proton ATPase and cytoskelton in osteoclasts." FEBS Letters. 401. 207-212 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakamura, N.他3名: "Vam2/Vps4lp and Vam6/VPs39p are components of a protein complex on thc vacuolar membrane and involved in the vacuolar assembly in the yeast Saccharomyces cerevisiae" J.Biol.Chem.272. 11344-11349 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakamura, N.他3名: "Acidification inside the vacuole is required for degradation of autopahagosomes in the yeast Saccharomyces cerevisiae." J.Biochem.121. 338-344 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nose, Y.他5名: "Specific increase in thc number of vanacdium-containing blood cells by some ionophores-ATPase in the ascidian Ascidia sydneiensis samea." Zool.Sci.14. (197)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sato, K.&Nakano, A.: "Rerlp as common machinery for the endoplasmic reticulum localization of membrane proteins." roc.Natl.Acad.Sci.U.S.A.94. 9693-9698 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sato, M.H.and Wada, Y.: "Universal template plasmid for introduction of the triple HA-epitope sequence into the cloned genes." BioTechniques. 23. 254-256 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sato, M.H.他7名: "The AtVAM3 encodes a syntaxin related molecule implicated in the vacuolar assembly in Arabidopsis thaliana.." J.Biol.Chem.272. 24530-24535 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sato, Y.他2名: "A tobacco cDNA encoding a homologue of N-ethylmaleimide sensitive fusion protein(NSF)from tobacco." Plant Physiol.113. 1464 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Scott, S.V.他3名: "Aminopeptidase I is targeted to the vacuole by a nonclassical vescicular mechanism." J.Cell Biol.138. 37-44 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimada, T.他3名: "A pumpkin 72-kDa membrane protein of precursor-accumulaing vesicles has characteristics of a valcuolar sorting receptor." Plant Cell Physiol.38. 1414-1420 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shirahama, K.他2名: "Mutational anaysis of Cscl/Vps2p-involvement of endosome in regulation of the autophagy in yeast" Cell Struc.Func.22. 501-509 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shiratake, K.他3名: "Changes in H^+-pumps and a tonoplast intrinsie protein of vacuolar membranes during the development of pear fruit." Plant Cell Physiol.38. 1039-1045 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tabuchi, M.他7名: "Vacuolar protein sorting in fission yeast:cloning,biosynthesis,transport, and processing of carboxy peptidase Y from Schizosaccharomyces pombe." J.Bacteriol.179. 4179-4189 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tabuchi.M.他3名: "The Schizosaccharomyces pombe gmsl^+ gene encodes an UDP-galactose transporter homologue required for protein galactosylation." Biochem.Biophys.Res.Commun.232. 121-125 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takasu, A.他3名: "Analysis of substrate binding site and carboxyl terminal region of Vacuolar H^+ translocating pyrophosphatase of mung bean with peptide antibodies." J.Biochem.122. 883-889 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tomochika, K.他5名: "Vacuolar ATPase in mouse bladder epithelium is responsible for urinary acidification." FEBS Letters. 404. 61-64 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ueda, K.and Noguchi, T.: "The number and the distribution pattern of Golgi bodies in cells of Micrasterias(Conjugales,Chlorophyta)examined by fluorescence microscopy and by electron microscopy." Phycological Research.45. 133-139 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Uyama.T.他4名: "Finding of the same antigens in the Polychaete,Pseudopotamilla occelata,as those in the vanadium-rich ascidian, Ascidia sydneiensis samea." Zool.Sci.14. 43-47 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Wada, Y.他3名: "Vam3p.a new member of syntaxin related protein,is required for vacuolar assembly in the yeast Saccharomyces cerevisiae." J.Cell Sci.110. 1299-1306 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamada, H.他2名: "L-Aspartate-evoked inhibition of melatonin production in rat pineal glands." Neurosci.Lett.228. 103-106 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamada, H.他7名: "Functional expression of a GLT-1 type Na^+-dependent glutamate transporter in rat pinealocytes." J.Neurochem.69. 1491-1498 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yatsushiro, 他5名: "L-Aspartate but not the D-form is secreted through microvesicle-mediated exocytosis and is sequestered through Na^+-dependent transporter in rat pinealocytes." J.Neurochem.69. 340-347 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kato, A.他5名: "Glyoxysomal malate dehydrogenase in pumpkin:cloning of a cDNA and functional analysis of its prosequence." Plant Cell Physiol.39. 184-195 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mimura, T.他2名: "Control of phosphate transport across the plasma membrane of Chara corallina" Journal of Experimental Botany. 49. 13-19 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Noda, T.&Ohsumi, Y.: "Tor,a phosphatidylinositol kinase homoiogue, controls autophagy in yeast." J.Biol.Chem.273. 3963-3966 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ueda, T.他5名: "AtGD12,a novel Arabidopsis gene encoding a Rab GDP dissociation inhibitor." Gene. 206. 137-143 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamamoto, A.他8名: "Bafilomycin Al prevents maturation of autophagic vacuolcs by inhibiting fusion betwee autophagosomes and lysosomes in rat hepatoma cell line,H-4-II-E cells." Cell Struct.Funct.23. 33-42 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ehara, M., Noguchi, T.and Ueda, K.: "Uptake of neutral red by the vacuole of a green alga, Micrasterial pinnatifida." Plant Cell Physiol.37. 734-741 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hayashi, M., Nose, Y., Uyama, T., Moriyama, Y.and Michibata, H.: "Rapid increase in number of vanadocytes in the vanadiumrich ascidian, Ascidia sydneiensis samea upon treatment of live animals with ammonium chloride." J.Exp.Zool.27. 1-7 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Higuchi, T., Hisada, H.and Maeshima, M.: "Complete nucleotide sequence of cDNA for an intrinsic protein in vacuolar membranes from radish roots (PGR 96-045)." Plant Physiol.111. 947 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kametaka, S., Matsuura, A., Wada, Y.and Ohsumi, Y.: "Structural and functional analyzes of APG5, a gene involved in autophagy in yeast." Gene. 178. 139-143 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Koumoto, Y., Tsugeki, R., Shimada, T., Kondo, M., Mori, H., Hara-Nishimura, I.and Nishimura, M.: "Isolation and characterization of a cDNA encoding mitochondrial chaperonin 10 from Arabidopsis thaliana by functional complementation of an E.coli" Plant J.10. 1119-1125 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Maeshima, M., Nakanishi, Y., Matsuura-Endo, C.and Tanaka, Y.: "Proton pumps of vacuolar membrane in growing plant cell" J.Plant Res.109. 119-125 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsumoto, H., Senoo, Y., Kasai, M.and Maeshima, M.: "Response of the plant root to aluminium stress : Analysis of the inhibition of the root elongation and changes in membrane function." J.Plant Res.109. 99-105 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mimura, T.sakano, Kand Shimmen, T.: "Studies on distribution, re-translocation and homeostasis of inorganic phosphate in barley leaves. Plant Cell & Environment, 193,11-320." Plant Cell & Environment1. 199. 1447-1454 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Moriyama, Y.: "Membrane energization by proton pumps is important for compartmentation of drugs and toxins : a new concept for active transport." J.Exp.Biol.199. 1447-1454 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Moriyama, Y.and Yamada, H.: "Vesicular monoamine transporter in microvesicles from bovine posterior pituitaries is immunologically similar to but distinct from the chromaffin granule counterpart in its sensivities to 1-methyl-4-phenylpyridinium and hista" Biochem.Biophys.Res.Commun.221. 790-794 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Moriyama, Y., Yamamoto, A., Yamada, H., Tashiro, Yo.and Futai, M.: "Role of microvesiclies in the novel chemical transduction from endocrine cell" J.Biol.Chem.377. 155-165

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Moriyasu, Y.and Ohsumi, Y.: "Autophagy in tobacco suspension-cultured cells in response to sucrose starvation." Plant Physiology. 111. 1233-1241 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakano, A.: "Identification and characterization of extragenic suppressors of the yeast sec12ts mulation" J.Biochem. 120. 642-646 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Robinson, D.G., Haschke, H.P., Hinz, G., Hoh, B., Maeshima, M.and Marty, F.: "Immunological detection of tonoplast polypeptides in the plasma membrane of pea cotyledons." Planta. 198. 95-103 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sato, M., Sato, K.and Nakano, A.: "Endoplasmic reticulum localization of Sec12p is achieved by two mechanisms : Rer1p-dependent retrieval that requires the transmembrane domain and Rer1p-independent retention that involves the cytoplasmic domain." J.Cell Biol.134. 279-293 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Scott, S.V., Hefner-Gravink, A., Morano, K.A., Noda, Y., Ohsumi, Y.and Klionsky, D.J.: "Cytoplasm-to-vacuole targeting and autophagy employ the same machinery to deliver proteins to the yeast vacuole." Proc.Natl.Acad.Sci.USA. 93. 12304-12308 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shirahama, K., Yazaki, Y., Sakano, K., Wada, Y.and Ohsumi, Y.: "Vacuolar function in the phosphate homeostasis of the yeast Saccharomyces cerevisiae." Plant Cell Physiol.37. 1090-1093 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ueda, T., Matsuda, N., Toyoaki Anai, Tsukaya, H., Uchimiya, H.and Nakano, A.: "An Arabidopsis gene isolated by a novel method for detecting genetic interaction in yeast encodes the GDP dissociation inhibitor of Ara4 GTPase." Plant Cell. 8. 2079-2091 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Wada, Y., Ohsumi, Y., Kawai, E.and Ohsumi, M.: "Mutational analysis of Vam4/Ypt7p function in the vacuolar biogenesis and morphogenesis in the yeast, Saccharomyces cerevisiae." Protoplasma. 191. 126-131 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamada, H., Moriyama, Y., Maeda M.and Futai.M.: "Transmembrane topology of Escherichia coli H+-ATPase (ATP synthase) subunit a." FEBS Letters. 390. 34-40 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamada, H., Yamamoto, A., Takahashi, M., Kumon, K.and Moriyama, Y.A: "The L-type Ca2+ channel is involved in microvesicle-mediated glutamate exocytosis from pinealocytes." J.Pineal Research. 21. 165-174 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamada, H., Yamamoto, A., Yodosawa, S., Kozaki, S., Takahashi, M., Morita, M., Michibata, H., Furuichi, T., Mikoshiba, K.and Moriyama, Y.: "Microvesicle-mediated exocytosis of glutamate is a novel paracrine-like signal transduction mechanism and inhibit" J.Pineal Research. 21. 175-191 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamanushi, T., Hirata, A., Oka, T.and Nakano, A.: "Characterization of yeast sar1 temperature-sensitive mutants, which are defective in protein transport from the endoplasmic reticulum." J.Biochem.120. 452-458 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshioka, Y., Takahashi, Y., Matsuoka, K., Nakamura, K., Koizumi, J., Kojima, M.and Machida, Y.: "Transient gene expression in plant cells mediated by Agrobacteium tumefaciens : aplication to" Plant Cell Physiol.37. 782-789 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Baba, M., Osumi, M., Scott, S.V., Klionsky, D.J.and Ohsumi, Y.: "Two distinct pathways for targeting proteins from the cytoplasm to the vacuole/lysosome." j.cell Biol.139. 1687-1695 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Beilby, M.J., Mimura, T.and Shimmen, T.: "Perfusion of charaphyte cells : A critical analysis of the method." Journal of Experimental Botany. 48. 157-172 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fleurat-lessard, P., Fragne, N., Maeshima, M., Ratajczak, R., Bonnemain, J.L.and Martioia, E.: "Increased expression of vacuolar aquaporin and H+-ATPase related to motor cell function in Mimo" Plant Physiol.114. 827-834 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Funakoshi, T., Matsuura, A., Noda, T.and Ohsumi, Y.: "Analyzes of APG13 gene involved in the autophagy in yeast, Saccharomyces cerevisiae." Gene. 192. 207-213 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hara-Nishimura, I.: "Asparaginyl endopeptidase." In Handbook of Proteolytic enzymes, edited by A.J.Barrett (Academic Press, London, UK). (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takasu, A., Nakanishi, Y., Yamauchi, T.and Maeshima, M.: "Analysis of substrate binding site and carboxyl terminal region of vacuolar H+ translocating pyrophosphatase of mung bean with peptide" J.Biochem.122. 883-889 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tomochika, K., Shinoda, S., Kumon, H., Mori, M., Moriyama, Y.and Futai, M.: "Vacuolar ATPase in mouse bladder epitheliumis resonsible for urinary acidification." FEBS Letters. 404. 61-64 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ueda, K.and Noguchi, T.: "The number and the distribution pattern of Golgi bodies in cells of Micrasterias (Conjugales, Chlorophyta) examined by fluorescence microscopy and by electron mirosc" Phycological Research.45. 133-139 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Uyama, T., Nose, Y., Wuchiyama, J.Moriyama, Y.and Michibata, H.: "Findin of the same antigens in the Polychaete, Pseudopotamilla occelata, as those in the vanadium-rich ascidian, Ascidia sy" Zool.Sci.14. 43-47 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Wada, Y., Nakamura, N., Ohsumi, Y.and Hirata, A.: "Vam3p, a new member of syntaxin related protein, is required for vacuolar assembly in the yeast Saccharomyces cerevisiae." J.Cell Sci.110. 1299-1306 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamada, H., Yamaguchi, A.and Moriyama, Y.: "L-Aspartateevoded inhibition of melatonin production in rat pineal glands." Neurosce.Lett.228. 103-106 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamada, H., Yatsushiro, S., Yamamoto, A., Hayashi, M., Nishi, T., Futai, M., Yamaguchi, A.and Moriyama, Y.: "Functional expression of a GLT-1 type Na+-dependent glutamate transporter in rat pi" J.Neurochem.69. 1494-1498 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yatsushiro, S., Yamada, H., Kozaki, S., Kumon, H., Michibata, H., Yamamoto, A.and Moriyama, Y.: "L-Aspartate but not the D-form is secreted through microvesicle-mediated exocytosis and is sequ" J.Neurochem.69. 340-347 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hara-Nishimura, I,Kinoshita, T,Hiraiwa, N.and Nishimura, M.: "Vacuolar processing enzymes in protein storage vacuoles and lytic vacuoles." J.Plant Physiol. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hara-Nishimura, I., Shimada, T., Hatano, K., Takeuchi Y.and Nishimura M.: "Transport of storage proteins to protein-storage vacuoles is mediated by large precursor-accumulating vesicles." Plant Cell. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hayashi, M., Yamamoto, A., Yatsushiro, S., Yamada, H., Futai, M., Yamaguchi, A.and Moriyama, Y.: "Synaptic vesicle protein SV2B,but not SV2A,is predominantly expressed and associated with mi" J.Neurochem.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishio, S., Yamada, H., Yatsushiro, S., Okamoto, S., Yamaguchi, A.and Moriyama, Y.: "D-aspartate modulates neuroendocrine function in rat pinealocyte." J.Neurochem.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kato, A., Takeda-Yoshikawa, Y., Hayashi, M., Kondo, M., Hara-Nishimra, I.and Nishimura, M.: "Glyoxysomal malate dehydrogenase in pumpkin : cloning of a cDNA and functional analysis of its pros" Plant Cell Physiol.39. 186-195

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsuda, N.and Nakano, A.: "RMA1, an Araidopsis gene that suppresses the yeast sec 15 mutation, encodes a novel protein with a RING finger motif and a membrane anchor." Plant Cell Physiol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mimura, T., Reid, R.J.and Smith, F.A.: "Control of phosphate transport across the plasma membrane of Chara corallina." Journal of Experimental Botany. 49. 13-19 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hatano K., Shimada, T., Hiraiwa, N., Nishimura, M.and Hara-Nishimura, I.: "A rapid increase in the level of binding protein (BiP) is accompanied by synthesis and degradation of storage proteins in pumpkin cotyledons." plant Cell Physiol. 38. 344-351 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiraiwa, N., Nishimura, M.and Hara-Nishimura, I.: "Expression and activation of the vacuolar processing enzyme in Saccharomyces cerevisiae." plant J.12. 819-829 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiraiwa, N., Kondo, M., Nishimura, M.and Hara-Nishimura, I.: "Anaspartic proteinase is involved in breakdown of propeptides of storage proteins in protein-storage vacuoles of plants." Eur.J.Biochem.246. 133-141 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Inoue, K., Wada, Y., Nishimura, M.and Hara-Nishimura, I.: "Heterologous expression and subcellular localization of pumpkin seed tonoplast intrinsic proteins (TIP) in yeast cells." Plant Cell Physiol.38. 366-370 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kanda, T., Nose, Y., Wuchiyama, J., Uyama, T., Moriyaa Y.and Michibata, H.: "Identification of a vanadium-associated protein from the vanadium-rich ascidian, Ascidia sydneiensis samea." Zool.Sci.14. 37-42 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawauchi, K., Shibutani, K., Yaisawa, H., Kamata, H., Nakatsuji, S., Anzai, H., Yokoyama, Y., Ikegami, Y., Moriyama, Y.and Hirata, H.: "A possible immunosuppressant, cycloprodigiosin hydrochl" Biochem.Biophys.Res.Commun.237. 543-547 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Koide, Y., Hirano, H., Matsuoka, K.and Nakamura, K.: "The N-terminal propeptide of the precursor to sporamin acts as a vacuolar targeting signal even at the C-terminus of the mature part in to" Plant Physiol.114. 863-870 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsuoka, K., Higuchi, T., Maeshima, M.and Nakamura, K.: "A vacuolar-type H+-ATPase in non-vacuolar organelle is required for the sorting of soluble vacuolar protein precursors in tobacco cell" Plant Cell. 9. 533-546 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsuura, A., Wada, Y.and Ohsumi, Y.: "Apg1p, a novel protein kinase required for the autohagic rocess in Saccharomyces cerevisiae." Gene. 192. 245-250 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mimura, T., Mimura, M., Washitani-Nemoto, S., Sakano, K., shimmen, T.and Siripatanadilok, S.: "Efficient callus initiation from leaf of mangrove plant, Bruguiera sexangula in amino acid medium" Journal of Plant Research. 110. 25-29 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mimura, T., Mimura, M., Washitani-Nemoto, S.and Siripatanadilok, S.: "NaCl-dependent growth, ion content and regeneration of calluses initiated from the mangrove plant, Bruguiera sexangula." Journal of Plant Research. 110. 31-36 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Minami, Y., Takao, H., Kanafuji, T., Miura, K., Kondo, M., Hara-Nishimura, I., Nishimra, M.and Matsubara, H.: "b-Glucosidase in the Indigo plant : intracellular localization and tissue specif" Plant Cell Physiol.38. 1069-1074 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakamura, I., Takahashi, N., Udagawa, N., Moriyama, Y., Kurokawa, T., Jimi, E., Sasaki, T.and Tsuda, T.: "Failure of bone resorption in osteosclerotic (co/oc) mice is possibly due to the disso" FEBS Letters. 401. 207-212 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakamura, N., Hirata, A., Ohsumi, Y.and Wada, Y.: "Vam2/Vps41p and Vam6/Vps39p are components of a protein complex on the vacuolar membrane and involved in the vacuolar assembly in the yeast S" J.Biol.Chem. 272. 11344-11349 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakamura, N., Matsuura, A., Wada, Y.and Ohsumi, Y.: "Acidification inside the vacuole is required for degradation of autopahagosomes in the yeast Saccharomyces cerevisiae." J.Biochem.121. 338-344 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nose, Y., Hayashi, M., Wuchiyama, J., Uyama, T., Moriyama, Y.and Michibata, H.: "Specific increase in the number of vanadium-containing blood cells by some ionophores-ATPase in the ascidian Asc" Zool.Sci.14. (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Reid, R.J., Mimura, T.and Smith, F.A.: "A high affinigy Na-dependent phosphate uptake induced by P starvation in Chara." In Plant nutrition-for sustainable food production and environment. Ando T.et al.eds.Kluwer Academic Publishers. 137-138 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sato, K., Sato, M.and Nakano, A.: "Rer1p as common machinery for the endoplasmic reticulum localization of membrane proteins." Proc.Natl.Acad.Sci.U.S.A.94. 9693-9698 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sato, M.H.and Wada, Y.: "Universal template plasmid for introduction of the triple HA-epitope sequence into the cloned genes." Bio Techniques. 23. 254-256 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sato, M.H.Nakamura, N., Ohsumi, Y., Kouchi, H., Kondo, M., Hara-Nishimura, I., Nishimura, M.and Wada, Y.: "The AtVAM3 encodes a syntaxin related molecule implicated in the vacuolar assembly" J.Biol.Chem.272. 24530-24535 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sato, Y., Matsuoka, K.and Nakamura K.: "A tobacco cDNA encoding a homologue of N-ethylmaleimide sensitive fusion protein (NSF) from tobacco." Plant Physiol. 113. 1464 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Scott, S.V., Baba, M., Ohsumi, Y.and Kionsky, D.J.: "Aminopeptidase I is targeted to the vacuole by a nonclassical vescidular mechanism." J.Cell Biol.138. 37-44 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimada, T., Kuroyanagi, M., Nishimura, M.and Hara-Nishimura, I.: "A pumpkin 72-kDa membrane protein of precursor-accumulating vesicles has characteristics of a vacuolar sorting rceptor." Plant Cell Physiol.38. 1414-1420 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shirahama, K., Noda, T.and Ohsumi, Y.: "Mutational analysis of Csc1/Vps2p-involvement of endosome in regulation of the autophagy in yeast" cell Struc.Func.22. 501-509 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shiratake, K., Kanayama, Y., Maeshima, M.and Yamaki, s.: "Changes in H+pumps and a tonoplast intrinsic protein of vacuolar membranes during the development of pear fruit." Plant Cell Physiol.38. 1039-1045 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Smart, L.B., Vojdani, F., Maeshima, M.and Wilkins, T.A.: "Genes involved in osmoregulation during turgor-driven cell expansion pf developing cotton fibers are differentially regulated." Plant Physiol.116 (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tabuchi, M., Iwaihara, O., Ohtani, Y., Ohuchi, N., Sakurai, J., Morita, t., Iwahara, S.and Takegawa, K.: "Vacuolar protein sorting in fission yeast : cloning, biosynthesis, transort, and proce" J.Bacteriol.179. 4179-4189 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tabuchi, M., Tanaka, N., Iwahara, S.and Takegawa, K.: "The Schizosaccharomyces pombe gms1+gene encodes an UDP-galactose transporter homologue required for protein galactosylation." Biochem.Biophys.Res.Commun.232. 121-125 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakanishi, Y.and Maeshima, M.: "Molecular cloning of vacuolar H+-pyrophosphatase and its developmental expression in growing hypocotyl of mung bean." Plant Physiol. 116 (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noda, T.and Ohsumi, Y.: "Tor, a phosphatidylinositol kinase homologue, controls autohagy in yeast." J.Biol.Chem.273. 3963-3966

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noguchi, T., watanabe, H.and Suzuki, R.: "Effects of brefeldin A on the Golgi apparatus, the nuclear envelope, and the endoplasmic reticulum in a green alga, Scenedesmus acutus." Protoplasma. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takeuchi, M., Tada, M., Saito, C., Yashiroda, H.and Nakano, A.: "Isolation of a tobacco gene encoding Sar1 GTPase and analysis of its dominant mutations in vesicular traffic using a yeast comp" Plant Cell Physiol. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ueda, T., Yoshizumi, T., Anai, T., Matsui, M., Uchimiya, H.and Nakano, A.: "AtGDI2, a novel Arabidopsis gene encoding a Rab GDP dissociation inhibitor." Gene. 206. 137-143 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamada, H., Yatsushiro, S., Ishio, S., Hayashi, M., Nishi, T., Yamamoto, A., Futai, M., Yamaguchi, A.and Moriyama, Y.: "Metabotropic glutamate receptors negatively regulate melatonin synthesis" Journal of Neuroscience. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamamoto, A., Tagawa, Y., Yoshimori, T., Moriyama, Y., Masaki, R.and Tashiro, Y.: "Bafilomycin A1 prevents maturation of autophagic vacuoles by inhibiting fusion between autophagosomes and lysosome" Cell Struct.Funct.23. 33-42

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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