| Project/Area Number |
08304045
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理
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| Research Institution | Okazaki National Research Institutes |
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Principal Investigator |
WATANABE Masakatsu Okazaki National Research Institutes, National Institutte for Basic Biology, Associate Professor, 基礎生物学研究所, 助教授 (40124226)
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| Co-Investigator(Kenkyū-buntansha) |
OHMORI Masayuki Tokyo University, Graduate School of Arts and Sciences, Professor, 総合文化研究科, 教授 (80013580)
WADA Hajime Kyushu university, Faculty of Science, Associate Professor, 理学部, 助教授 (60167202)
SHIGA Kiyoshi Kumamoto University, Faculty of Medicine, Professor, 医学部, 教授 (40028527)
YASUI Akira Tohoku University,Institute of Development, Aging and Cancer, 加齢医学研究所, 教授 (60191110)
IINO Moritoshi Osaka City University,Faculty of Science, Associate Professor, 理学部, 助教授 (50176054)
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| Project Period (FY) |
1996 – 1998
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| Keywords | Blue light / Photoreceptor systems / Flavins / Algae / cAMP / Cryptochromes / Signal transduction systems / Ion channls |
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| Research Abstract |
Using mainly such algae as Euglena, cyanobacteria, and Chlamydomonas, molecular structures and functions of blue-light sensing photoreceptor-signal transuduction systems have been analyzed : (1) a novel blue-light photoreceptor protein has been identified for Euglena photomovement by biochemical extraction, subsequent molecular cloning and sequencing (Iseki, Matsunaga, Murakami, Shiga. Sugai, Hori. Takahashi, Watanabe) ; (2) blue-light insensitive mutants have been genarated in the cyanobacterium Synechocystfs sp. PCC 6803 using blue-1ight requiring heterotrophic growth response (Wada) ; (3) photo-signal transduction pathways in which adenylate cyclases are involved have been demonstrated for red/far-red and blue light sensing in cyanobacteria (Ohmori) ; (4) involvement of protein kinases has been phannacologically demonstrated in blue-light -induced activation of proton pumping in Chlorella (Kamiya) ; (5) the cryptochrome-mediated membrane depolarization in Arabidopsis protoplast cells proceeding shrinking has been ascribed to activation of anion channels and resultant efflux of chloride ions (Iino) ; (6) UV-A light, not blue light, has been shown action spectroscopically to control gametogenesis in Chlamydomonas (Saito, Matsuda, Watanabe) ; (7) mammalian cryptochromes have been molecular genetically shown to be essential as photoreceptors for light-mediated entraining of circadian rhythms in mice (Yasui).
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