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1998 Fiscal Year Final Research Report Summary

Study on development and clinical application of new bactericidal-biocompatible material for conserving infected pulp.

Research Project

Project/Area Number 08407063
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Conservative dentistry
Research InstitutionNiigata University

Principal Investigator

IWAKU Masaaki  Niigata University, School of Dentistry, Professor, 歯学部, 教授 (70013927)

Co-Investigator(Kenkyū-buntansha) YOSHIBA Kunihiko  Niigata University, Dental Hospital, Assistant Professor, 歯学部附属病院, 助手 (30220718)
YAMAGA Masahiro  Niigata University, School of Dentistry, Associate Professor, 歯学部, 助手 (10260548)
AYUKAWA Yukio  Niigata University, School of Dentistry, Associate Professor, 歯学部, 助手 (00183145)
KOTA Kohichi  Niigata University, School of Dentistry, Associate Professor, 歯学部, 助教授 (90018755)
HOSHINO Etsuro  Niigata University, School of Dentistry, Professor, 歯学部, 教授 (90124619)
Project Period (FY) 1996 – 1998
KeywordsInfected Pulp / Obligate Anaerove / Metoronidazol / alpha-Tricalcium phosphate / Biocompatibility / Antibacterial Drug / Hard Tissure Barrier / Pulp Conservation
Research Abstract

This study was projected for development and clinical application of the most effective new bactericidal-biocompatible material to conserve the infected pulp tissue.
Following results were obtained :
1. RFLP Analysis of 16S rDNA from Pseudolamibacter alactolyticus.
In this study, we examined the restriction fragment-length polymorphism(RFLP) of the 16S-rDNA amplified by poymerase chain reaction(PCR) to assess phylogenetic heterogeneity of this bacterial species. In conclusion, these data suggested that P.alactolyticus may not be a phylogenetically homogeneous species.
2. Artificial Bacterial Bioflims and Confocal Laser Scanning Microscopic Analysis.
To elucidate the structure and the function of biofilms, mucoid-type Pseudomonas aeruginosa organisms were cultured in broth media supplied. The biofilm structure was visualized by fluorescent staining and were exposed to ciprofloxacin.
These results indicate that the confocal laser scanning microscopical analysis of artificial biofilms was useful to elucidate bacterial functions in bioflims, and may lead to new quantitative estimation of bactericidal efficacy of natibacterial drugs.
3. Tooth development is regulated by epithelial-mesenchymal interactions mediated by the basement membrane. The expression and localization of laminin-5 were temporally and spatially regulated during mouse tooth development. The temporo-spatial changes in basement membrane composition might be related with tooth morphogenesis and cytodifferentiation.
4. The amount of fluoride release from fluoridated restorative materials, fluoride uptake into enamel and dentine, and effect of fluoride on acid resistance of the tooth structures, were evaluated Fluoride releasing materials was effective for prevention of secondary caries.
5.This work is now in progress

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Nagako Yoshiba et al: "Expression and localization of laminin-5 subunits in the mouse incisor" Cell and Tissue Research. 292巻. 143-149 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kunihiko Yoshiba et al: "Expression and localization of laminin-5 subunits during mouse tooth development" Developmental Dynamics. 211巻. 164-176 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nagako Yoshiba et al: "Immunohistochemical Localization of Class II Antigens and Nerve Fibers in Human Carious Teeth : HLA-DR Immunoreactivity in Schwann Cells" Arch.Histol.Cyto.61巻. 343-352 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 韓 臨麟 et al: "接着性コンポジットレジンにおけるフッ素含有フィラー配合ボンディング材に関する研究 -フッ素徐放性,歯質への取り込みおよびその歯質耐酸性に及ぼす影響-" 日歯保存誌. 41巻. 690-687 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kunihiko Yoshiba: "Expression and localization of laminin-5 subunits during mouse tooth development." Developmental Dynamics. 211. 164-176 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nagako Yoshiba: "Expression and localization of laminin-5 subunits in the mouse incisor." Cell and Tissue Research. 292. 143-149 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nagako Yoshiba: "Immunohistochemical Localization of Class II Antigens and Nerve Fibers in Human Carious Teeth : HLA-DR Immunoreactivity in Schwann Cells" Arch.Histol.Cyto.Vol.61 (4). 343-352 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Linlin Han: "Study on Fluoride Releasing Bonding Resin System-Amount of Fluoride Release, Upatake, Effect on Acid Resistance of Fluoridated Tooth Structures-" Japan.J.Conserv.Dent.Vol.41 (4). 690-697 (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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