1997 Fiscal Year Final Research Report Summary
Mechanism of gene expression by two-component regulatory systems and sigma factors
| Project/Area Number |
08454245
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
遺伝
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| Research Institution | Osaka University |
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Principal Investigator |
MAKINO Kozo Research Institute for Microbial Diseases : Osaka University, Associate Professor, 微生物病研究所, 助教授 (20181620)
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| Project Period (FY) |
1996 – 1997
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| Keywords | Two-comonent / PhoB / sigma factor / RNA polymerase / cell cycle / stress response / rpoS / DNA binding |
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| Research Abstract |
The important results in this project are as belows ;
1) We determined the molecular structure of PhoB protein that is a member of the two-component regulatory systems. Especially we determined the regions of PhoB involved in the interaction with RNA polymerase and DNA binding.
2) By a systematic DNA sequencing of the E.coli hole genome, we revealed the existence of new two-component regulatory genes.
3) We revealed the existence of two-component regulatory genes is Schzosaccharomyces pombe. These genes were related to the cell cycle and stress responses.
4) We have done the molecuar cloning and functional analysis of the rpoS gene of Vibrio cholerae, which encodes the stational-specific sigma factor of RNA polymerase.
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Research Products
(16 results)
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[Publications] Oshima T., H.Aiba, T.Baba, T.Fujita, K.Hayashi, A.Honjo, K.Ikemoto, T.Inada, T.itoh, M.Kajihara, K.Kanai, K.Kashimoto, S.Kimura, M.Kitagawa, K.Makino, S.Masuda, T.Miki, K.Mizobuchi, H.Mori, K.Motomura, Y.Nakamura, H.Nashimoto, Y.Nishio, N.Saito, G.Sampei, Y.Seki, H.Tagami, K.Takemoto, C.Wada, Y.Yamamoto, M.Yano and T.Horiuchi.: "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res.3 (Supplement, 211-223). 137-155 (1996)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Aiba H., T.Baba, K.Hayashi, T.Inada, K.Isono, T.Itoh, H.Kasai, K.Kashimoto, S.Kimura, M.Kitakawa, M.Kitagawa, K.Makino, T.Miki, K.Mizobuchi, H.Mori, T.Mori, K.Motomura, S.Nakade, Y.Nakamura, H.Nashimoto, Y.Nishio, T.Oshima, N.Saito, G.Sampei, Y.Seki, S.Sivasundaram, H.Tagami, J.Takeda, K.Takemoto, Y.Takeuchi, C.Wada, Y.Yamamoto and T.Horiuchi.: "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." DNA Res.3 (Supplement, 435-440). 363-377 (1996)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Itoh T., H.Aiba, T.Baba, K.Hayashi, T.Inada, K.Isono, H.Kasai, S.Kimura, M.Kitakawa, K.Makino, T.Miki, K.Mizobuchi, H.Mori, T.Mori, K.Motomura, S.Nakade, Y.Nakamura, H.Nashimoto, Y.Nishio, T.Oshima, N.Saito, G.Sampei, Y.Seki, S,Sivasundaram, H.Tagami, J.Takeda, K.Takemoto, C.Wada, Y.Yamamoto and T.Horiuchi.: "A 460-kb DNA sequence of the Escherichis coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." DNA Res.3 (Supplement, 441-445). 379-392 (1996)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Yamamoto Y., H.Aiba, T.Baba, K.Hayashi, T.Inada, K.Isono, Takesgi Itoh, S.Kimura, M.Kitakawa, K.Makino, T.Miki, N.Mitsuhashi, K.Mizobuchi, H.Mori, S.Nakade, Y.Nakamura, H.Nashimoto, T.Oshima, S.Oyama, N.Saito, G.Sampei, Y.Satoh, S.Sivasundaram, H.Tagami, H.Takahashi, J.Takeda, K.Takemoto, K.Uehara, C.Wada, S.Yamagata and T.Horiuchi.: "Construction of a contiguous 874-kb sequence of the Escherichia coli K-12 genome corresponding to the 50.0-68.8 min on the linkage map and analysis of its sequence factures." DNA Res.4 (Supplement, 169-178). 91-113 (1997)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Makino, K., K.Ishii, T.Yasunaga, M.Hattori, K.Yokoyama, C.Yutsudo, Y.Kubota, Y.Yamaichi, T.Iida, K.Yamamoto, T.Honda, C.-G.Han, E.Ohtsubo, M.Kasamatsu, T.Hayashi, S.Kuhara and H.Shinagawa.: "Complete nucleotide sequences of 93-kb and 3.3-kb plasmids of an enterohemorrhagic Escherichia coli O157 : H7 derived from Sakai outbreak." DNA Res.(in press).
Description
「研究成果報告書概要(欧文)」より
