Studies on the action of apoptosis inducing factor derived from bovine colostrum

1997 Fiscal Year Final Research Report Summary

• Project/Area Number: 08456136
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied animal science
• Research Institution: TOHOKU UNIVERSITY
• Principal Investigator: SASAKI Yasuyuki TOHOKU UNIVERSITY,FACULTY OF AGRICULTURE,PROFESSOR, 農学部, 教授 (90005637)
• Co-Investigator(Kenkyū-buntansha): HAGINO Akihiko TOHOKU UNIVERSITY,FACULTY OF AGRICULTURE,RESEARCH ASSOCIATE, 農学部, 助手 (80156249) ; KATOH Kazuo TOHOKU UNIVERSITY,FACULTY OF AGRICULTURE,ASSOCIATE PROFESSOR, 農学部, 助教授 (60091831)
• Project Period (FY): 1996 – 1997
• Keywords: IGF-I / colostrum / apoptosis / tyrosine kinase

Research Abstract

We found a cell-growth inhibiting factor in partially purified fraction from bovine colostrum using an ion-exchange chromatography. The factor induced apoptosis in mouse LM fibroblasts. It is well known that bovine colostrum contains IGF-I,so we measured IGF-I concentrations of the fraction. The fraction contained about 500ng/mg protein of IGF-I.Then, effects of IGF-I on cell proliferation were determined using three cell lines, LM,L6, and Balb/c 3T3 cells. To our surprise, IGF-I induced cell death of LM cells, while it stimulated proliferation of L6 and Balb/c 3T3 cells in low FCS condition. Herbimycin A,a tyrosine kinase inhibitor, inhibited cell death induced by IGF-I in LM cells.
These data suggests that IGF-I induces cell death of LM cells and the action involves activation of tyrosine kinase.