• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1998 Fiscal Year Final Research Report Summary

Viral and host factors contributing to the neuro-virulence of rabies virus.

Research Project

Project/Area Number 08456150
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionGifu University

Principal Investigator

MINAMOTO Nobuyuki  Gifu University・Faculty of Agriculture, Professor, 農学部, 教授 (10144007)

Co-Investigator(Kenkyū-buntansha) ATOJI Yasurou  Gifu University・Faculty of Agriculture, Associate Professor, 農学部, 助教授 (90151084)
KOMORI Seiichi  Gifu University・Faculty of Agriculture, Professor, 農学部, 教授 (70195866)
SUGLYAMA Makoto  Gifu University・Faculty of Agriculture, Associate Professor, 農学部, 助教授 (80196774)
Project Period (FY) 1996 – 1998
KeywordsRabies virus / Glycoprotein gene / Nucleotide substitution / Revertant / Deduced amino acid sequence / Whole-cell patch clamp / Na^+ channel / Ultra structure
Research Abstract

In this study, to definite the molecular bases of neurovirulence in rabies virus, we have analyzed and characterized the pathogenic factors in the both virion and cells. The results run as follows :
1) The RC-HL strain lacking the capacity to kill adult mice was passaged intracerebrally 22 times in suckling mice. Each passaged virus failed to regain lethal virulence for adult mice, although the viruses were increased to level of mouse pathogenicity.
2) The revertant strain acquired partially virulence had each one amino acid substitution in G and M proteins. and one nucleotide substitution in non-coding region of 3' end in M gene when compared with full genome of the RC-HL strain. These changed positions of a nucleotide and two amino acids may necessary for mouse virulence.
3) A comparative analysis of antigenic variants selected in the presence of virus-neutralizing monoclonal antibody (N-MAb) with cell-fusion inhibition activity, revealed that fusion activity corresponded to cystine at position 35 of the G protein.
4) Any neutralization-resistant mutants by a N-MAb recognized an amino acid at position 333 of the (3 protein that related to virulence of rabies virus could not be selected from virulent virus, the Nishigahara strain, suggesting that arginine at the position is necessary for replication of virulent rabies virus.
5) It was suggested that infection of mouse neuroblastoma NA cells with rabies virus causes reduction of functional expression of ion channels responsible for Na^+ current and delayed rectifier K^<1+> current, and provided evidence for possible involvement of the change in membrane properties in the pathogenesis of rabies disease.

  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Gamoh,K.,: "Use of ELISA for in vitro potency test of rabies vaccine for animal use." Biologicals. 24. 95-101 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Atoji,Y.,: "Distribution of neurotensin-containig neurons in the central nervous system of the pigeon and the chiken" Journal of Comparative Neurology. 375. 187-211 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Anzai,J.,: "Identification of a phosphatase-sensitive epitope of rabies virus nucleo-protein which is recognized by a monoclonal antibody5-2-26." Microbiology & Immunology. 41(3). 229-240 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawai,A.,: "Monoclonal antibody #5-2-26 the phosphatase-sensitive epitope of rabies virus nucleoprotein." Microbiology & Immunology. 41. 33-42 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sugiyama,M.,: "A new competitive enzyme-linked immunosorbent assay demonstrates adequate immune levels to rabies virus in compulsorily vaccinated Japanese domestic dogs." Clin.Diag.Lab.Immunol.,. 4. 727-730 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Luo,T.R.,: "A virus-neutralizing epitope on the glycoprotein of rabies virus that contains Trp251 is a linear epitope." Virus Research. 51. 35-41 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Luo,T.R.,: "Antigenic and functional analyses of glycoprotein of rabies virus using monoclonal antibodies." Microbiol.Immunol.42. 187-193 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iwata,M.,: "Modification of membrane currents in mouse neuroblastoma cells following infection with rabies virus" British Journal of Pharmacology. in press. (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ito,N.,: "Molecular epidemiology of rabies in Thailand" Microbiology & Imunology. in press. (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiraga,S.,: "Expression of the rabies virus Glycoprotein by recombinant baculovirus and its fusion activity." Microbiology & Immunology. 発表予定.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Minamoto,N.,: "The molecular basis for rabies virus neurovirulence in Mouse" Vaccine. 発表予定.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Minamoto,N.,: "ldentification of fusogenic domain in rabies virus glycoprotein" Virus Research. 発表予定.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Gamoh, K.: "Use of ELISA for in vitro potency test of rabies vaccines for animal use." Biologicals. 24. 95-101 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Atoji, Y.: "Distribution of neurotensin-containing neurons in the centralnervous system of the pigeon and the chiken." J.Comp.Neurol.375. 187-211 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Anzai, J.: "Identification of a phosphatase-sensitive epitope of rabies virus nucleoprotein which is recognized by a monoclonal antibody 5-2-26." Microbiol.Immunol.41. 229-240 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawai, A.: "Monoclonal antibody 5-2-26 the phosphatase-sensitive epitope of rabies virus nucleoprotein." Microbiol.Immunol.41. 33-42 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sugiyama, M.: "A new competitive enzyme-linked immunosorbent assay demonstrates adequate immune Jevels to rabies virus in compulsorily vaccinated Japanese domestic dogs." Clin.Diag.Lab.Immunol.4. 727-730 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Luo, T.R.: "A virus-neutralizing epitope on the glycoprotein of rabies virus that contains Trp251 is a linear epitope." Virus Res.51. 35-41 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Luo, T.R.: "Antigenic and functional analyses of glycoprotein of rabies virus using monoclonal antibodies" Microbiol.Immunol.42. 187-193 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwata M.: "Modification of membrane currents in mouse neuroblastoma cells following infection with rabies virus." British J.Pharmacol.(in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ito, N.: "Molecular epidemiology of rabies in Thailand." Microbiol.Immunol.(in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiraga, S.: "Expression of the rabies virus Glycoprotein by recombinant baculovirusand its fusion activity." Microbiol.Immunol.(in preparation).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Minamoto, N.: "The molecular basis for rabies virus neurovirulence in Mouse." Vaccine. (in preparation).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Minamoto, N.: "Identification of fusogenic domain in rabies virus glycoprotein." Virus Res.(in preparation).

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1999-12-08  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi