1998 Fiscal Year Final Research Report Summary
GENE THERAPY FOR CEREBRAL VASOSPASM ASSOCIATED WITH SUBARACHNOID HEMORRHAGE
| Project/Area Number |
08457367
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | OKAYAMA UNIVERSITY |
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Principal Investigator |
ASARI Shoji OKAYAMA UNIVERSITY,MEDICAL SCHOOL,PROFESSOR, 医学部, 教授 (40175857)
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| Co-Investigator(Kenkyū-buntansha) |
NINOMIYA Yoshifumi OKAYAMA UNIVERSITY MEDICAL SCHOOL,ROFESSOR, 医学部, 教授 (70126241)
TOMITA Susumu OKAYAMA UNIVERSITY MEDICAL SCHOOL,ASSISTANT ROFESSOR, 医学部, 助手 (90237115)
DATE Isao OKAYAMA UNIVERSITY UNIVERSITY MEDICAL SCHOOL,HOSPITAL,ASSISTANT ROFESSOR, 医学部附属病院, 助手 (70236785)
MATSUMOTO Kengo OKAYAMA UNIVERSITY MEDICAL SCHOOL,ASSOCIATE PROFESSOR, 医学部, 助教授 (10190521)
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| Project Period (FY) |
1996 – 1998
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| Keywords | CEREBRAL VASOSPASM / SUBARACHNOID HEMORRHAGE / GENE THERAPY |
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| Research Abstract |
We have investigated the pathophysiology of cerebral vasospasm associated with subarachnoid hemorrhage using molecular biology techniques, that is, in vivo study using animal models of subarachnoid hemorrhage, We have shown that cistemal administration of antisenseoligonucleotide for entothelin could releave the early stage of cerebral vasospasm, administration of antisenseoligonucleotide for collagen reduced the degree of cerebral vasospasm and cisternal administration of antisenseoligonucleotide for endothelin-liposome complex suppressed cerebral vasospasm, We have also shown that decoy-liposome complex administration of NF-kB could improve the degree of vasospasm, suggesting that inflamation plays an important role in cerebral vasospasm.
We evaluated vectors for gene delivery. In vitro study showed that adenoviral vector with Lac Z gene could be transfected by cultured endothelial cells and smooth muscle cells exposed to hemolysate. In vivo study also showed that adenoviral vector could induce be used for gene transfection to cerebral vessels.
The relationship between the cerebral vasospasm and aging was also investigated. The response of cerebral vessels to papaverin chloride was
decreased with aging.
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