1998 Fiscal Year Final Research Report Summary
EFFECTS OF VOLATILE ANESTHETICS ON SODIUM/CALCIUM EXCHANGER ACTIVITY IN ISCHEMIC-REPERFUSION INJURY
| Project/Area Number |
08457404
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | YAMANASHI MEDICAL UNIVERSITY |
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Principal Investigator |
KUMAZAWA Teruo Yamanashi Medecal University, Dept.of Anesthesiology, Professor, 医学部, 教授 (10092404)
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| Co-Investigator(Kenkyū-buntansha) |
NONAKA Akihiko Yamanashi Medecal University, Dept.of Anesthesiology, Assistant Professor, 医学部, 講師 (90228338)
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| Project Period (FY) |
1996 – 1998
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| Keywords | MYOCYTES / CALCIUM / SODIUM EXCHANGER / HALOTHANE |
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| Research Abstract |
We studied that effects of volatile anesthetics on sodium/calcium exchanger activity under ischemic-reperfusion injury. In this study, we examined Na+/Ca+ exchange activity, Ca2+ transients, and contractility in isolated rabbit ventricular myocytes. Intracellular Na ([Na+]i) and Ca2+ concentration ([Ca2+]i) were measured with the fluorescent Na indicators SBFL and fluo 3, respectively, and contractility was assessed from changes in cell shortening during field stimulation. Experiments were performed at extracellular pH 7.4 in the presence of HEPES buffer.
Our findings demonstrate that I) halothane significantly decreased Ca2+ transients and contractility, 2) ischemic-reperfusion caused increases in [Ca2+]i and [Na+]i, 3) halothane attenuate the magnitude in increases in [Ca2+]i during reperfusion, 4) halothane did not alter the changes in [Na+]i during ischemic period.
It is suggested that a reduction of excessive intracellular calcium after reperfusion caused by halothane may be beneficial on myocardial function following reperfusion in the intact rabbit myocardium.
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