1997 Fiscal Year Final Research Report Summary

The Role of Oral Immunotorelance in the Pathogenesis of Periodontal Disease

Research Project

Project/Area Number	08457516
Research Category	Grant-in-Aid for Scientific Research (B)
Allocation Type	Single-year Grants
Section	一般
Research Field	Conservative dentistry
Research Institution	Fukuoka Dental College
Principal Investigator	NAGAI Atsushi Fukuoka Dental College, Department of Periodontics and Endodontics, Associate Professor, 歯学部, 助教授 (70252989)
Co-Investigator(Kenkyū-buntansha)	FUJIMURA Tetsuyuki Fukuoka College of Health Sciences, Department of Dental Hygene, Associate Profe, 歯科衛生士学科, 助教授 (70269033)
Project Period (FY)	1996 – 1997
Keywords	periodontopathic bacteria / experimantla periodontitis / Porphyromonas gingivalis / oral immunotolerance / periodontal infection
Research Abstract	To clarify roles of oral immunotorelance caused byu transmission of periodontal bactereia to intestinal microflora in the pathogenesis of periodontal disease, the present study aimed to establish an experimental periodontitis model mouse. Experimental periodontitis was induced by ligation of silk thread which were soaked in P.gingivalis culture memdia around molar of C3H/HeN mouse. Habilitation of in-noculated P.gingivalis was confirmed by AP-PCR mothod. Development of experimental periodontitis were confirmed by alveolar bone loss at the innoculated sites. Habilitation of innoculated P.gingicalis was suggested by the findings that P.gingivalis of the sama amplitype as innoculated was found in the intestinal microflora. This finding strongly suggest that patients with periodontitis in established phase develop immunotolerant to periodontal bacteria habitating in the intertinal microflora.