1997 Fiscal Year Final Research Report Summary
Defense Mechanism of cells from stresses
| Project/Area Number |
08458235
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | The Tokyo Metropolitan Institute of Medical Science |
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Principal Investigator |
MATSUMOTO Seiji RINSHOKEN,Cell Biology, Research Associate, 細胞生物学研究部門, 研究員 (40190532)
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| Co-Investigator(Kenkyū-buntansha) |
AIZAWA Hiroyuki RINSHOKEN,Cell Biology, Research Associate, 細胞生物学研究部門, 研究員 (90221704)
IIDA Kazuko RINSHOKEN,Cell Biology, Research Associate, 細胞生物学研究部門, 研究員 (40151229)
MOTIYAMA Kenji RINSHOKEN,Cell Biology, Research Associate, 細胞生物学研究部門, 研究員 (00250217)
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| Project Period (FY) |
1996 – 1997
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| Keywords | stress protein / heat shock protein / stress response / cell defense mechanism / HSP90 / molecular chaperone / cofilin / Actin |
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| Research Abstract |
1-1. We found taht heated HSP90 binds and protects denatured firefly luciferase from irreversible aggregation. Luciferase can be released from the complexes when post-incubated in HSP70, HSP40 and another chaperone contained in reticulocyte lysate.
1-2. We found taht monoclonal antibodies specific to the N-terminus of HSP90 bound to both ends of HSP90 dimers. This indicates that HSP90 monomers align side-by-side in an antiparallel fashion and form dimers through their C-termini.
1-3. In the budding yeast, the expression level of Hsc82 was found to be reversibly proportional to the degree of stress tolerance.
2-1. We have determined tertiary structure of destrin, a cofilin-related protein, by NMR.The structure is similar to that of a unitary segment of gelsolin. The structure provides molecula basis for Ca^<2+> -independent binding of cofilin to actin.
2-2. Overexpression of cofilin in Dictyostelium cells induced a number of actin bundles. This was suggested to be a result of transformation from actin meshwork to bundles by cofilin-induced severing of F-actin. We further demonstrated that the bundles contracted in response to osmotic stress.
2-3. We found that cofilin was inactivated by phosphorylation of the Ser-3
2-4. A multicopy suppressor to a cofilin-ts mutant in Saccharomyces cerevisiae was isolated. The suppressor gene SCF1 encodes a WD40-repeat protein which is identidal to Aip1 previously described as an actin-intereating protein. SCF1 is not essential for cell viability.
2-5. Biochemical evidence has been obtained that Dictyostelium Aip1 binds to F-actin and seisitizes it to destruction by cofiin.
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[Publications] Hatanaka, H., Ogura, K., Moriyama, K., Ichikawa, S., Yahara, I.And Inagaki, F.: "Tertialry structure of destrim and structural similarity between two actin-regulating protein families." Cell. 85. 1047-1055 (1996)
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「研究成果報告書概要(欧文)」より
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[Publications] Emoto, K., Kobayashi, T., Yamaji, A., Aizawa, H., Yahara, I., Inoue, K., and Umeda, M.: "Redistribution of phosphatidylethanolamine at the cleavage furrow of diving cells during cytokinesis." Proc.Natl.Acad.Sci.USA. 93. 12867-12872 (1996)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Yahara, I., Aizawa, H., Moriyama, K., Iida, K., Yonezawa, H., Nishida, E., Hatanaka, N., and Ingaki, F.: "A role of cofilin/destrin in reorganization of actin cytoskeleton in response to stresses and cell stimuli." Cell Struct.Funct.21. 421-424 (1996)
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「研究成果報告書概要(欧文)」より
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[Publications] Kimura, Y., Rutherford, S.L., Miyata, Y., Yahara, I., Freeman, B.C., Yue, L., Morimoto, R.And Lindquist, S.: "Cdc37 is a molecular chaperone with specific functions in signal transduction." Genes & Dev.11. 1775-1785 (1997)
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「研究成果報告書概要(欧文)」より
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[Publications] Miyata, Y., Chambraud, B., Radanyi, C., Leclearc, J., Lebeau, M.-G., Renoir, J.-M., Shirai, J., Catelli, M.-G., Yahara, I., and Bauliew, E.-E.: "Phosphorylation of the immunesuppressant FK506-binding protein FKBP52 by casein kinase II : Regulation of HSP90-binding activigty of FKBP52." Proc.Natl.Acad.Sci.USA. 94. 14500-14505 (1997)
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「研究成果報告書概要(欧文)」より
