1997 Fiscal Year Final Research Report Summary
Study on the automated DNA profilling in paternity testing
| Project/Area Number |
08557034
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Legal medicine
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| Research Institution | Shinshu University |
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Principal Investigator |
FUKUSHIMA Hirofumi Shinshu Univ.Sch.Med., Professor, 医学部, 教授 (70135218)
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| Co-Investigator(Kenkyū-buntansha) |
ARAKURA Akikazu Shinshu Univ., Sch.Med., assistant, 医学部, 助手 (00283250)
OTA Masao Shinshu Univ., Sch.Med., lecturer, 医学部, 講師 (50115333)
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| Project Period (FY) |
1996 – 1997
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| Keywords | PCR / fluorescent / paternity testing / STR / polymorphism / DNA / minisatellite / microsatellite |
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| Research Abstract |
Based on the length and genomic distribution of the core repeat sequences, two systems are classified as minisatellites and microsatellites (or short tandem repeats). In particular, short tandem repeats (STRs) are abundant classes of polymorphic loci dispersed throughout the entire genome. Each STR locus usually consists of a limited number of the core repeats of 2-5 basepairs, which can easily be resolved by analytical gel electrophoresis. The Japanese and Chinese populations were studies for the ACTBP2 and D4S43 loci. In combination with the use of fluorescence-labeled primers employing different fluorescent dyes, STR loci with overlapping or identical allele size ranges can be analyzed together in the same lane of polyacrylamide gel, thus providing a rapid and accurate method for paternity testing and human identification. Automated DNA profilling by fluorescence-based technology, as well as its application, have been studied and validated for use in paternity testing routine.
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Research Products
(14 results)
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[Publications] Harashima, N., Liu, C., Katsuyama, Y., Ota, M., Hama, Y., Fukushima, H.: "Automated DNA typing of ACTBP2 : a comparsion of allelic ladder with GS 2500 as internal marker." Jpn J Legal Med.50. 27-29 (1996)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Harashima, N., Ota, M., Katsuyama, Y., Sugiyama, E., Liu, C.Y., Fukushima, H.: "The allele frequency distribution at VNTR locus D17S5 (YNZ22) in a Japanese population sample." Jpn J Legal Med.50. 237-240 (1996)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Katsuyama, Y., Harashima, N., Tsuchikane, A., Ota, M., Liu, C.Y., Zenda, H., Fukushima, H.: "Allele distribution of a VNTR polymorphism at D4S43 locus in the Japanese population." Jpn J Legal Med.50. 6-9 (1996)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Saito, S., Ota, S., Hashizume, K., Yamada, E., Kaneshige, T., Kinoshita, T., Hashimoto, M., Oguchi, H., Ishii, E., Fukushima, H.: "A new HLA-DQB1^<**>0306 allele sharing motifs from DQB1^<**>03032 and DQB1^<**>04 sequences." Tissue Antigens. 48. 580-585 (1996)
Description
「研究成果報告書概要(欧文)」より
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