1998 Fiscal Year Final Research Report Summary
Construction of mouse embryonic stem cells for the detection of gene mutation
| Project/Area Number |
08559016
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
広領域
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| Research Institution | National Institute of Health Sciences |
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Principal Investigator |
INOUE Tohru National Institute of Health Sciences, Division of cellular and molecular toxicology, director, 安全性生物試験研究センター・毒性部, 部長 (50100110)
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| Co-Investigator(Kenkyū-buntansha) |
SAGA Yumiko National Instituteof Health Seiences, Divesion of cellular and molecular toxicology, section chief, 安全性生物試験研究センター・毒性部, 室長 (50221271)
HIRABAYASHI Yoko National Instituteof Health Seiences, Divesion of cellular and molecular toxicology, senior researrcher, 安全性生物試験研究センター・毒性部, 主任研究官 (30291115)
TAKAGI Atsuya National Instituteof Health Seiences, Divesion of cellular and molecular toxicology, senior researrcher, 安全性生物試験研究センター・毒性部, 主任研究官 (00179417)
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| Project Period (FY) |
1996 – 1998
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| Keywords | Embryonic stem cell / developmental toxicity / gene mutation |
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| Research Abstract |
The mouse ES cells can be maintained in tissue culture with pluripotency. ES cells can also be induced to differentiate in vitro. These properties make it an excellent tool for the study of early embryogenesis and, potentially, teratogenesis in vitro. We have established genetic reporter systems in the ES cells for the detection of mutations. E.coli xanthine-guanine phosporibosyl transferase (XPRT) gene (gpt) was transfected to the HPRT- ES cells. After transfection with the gpt gene (pSV2-gpt), surviving cells producing XPRT can be selectively grown with xanthine as the sole precursor for guanine nucleotide formation in a medium containing inhibitors (aminopterin and mycophenolic acid) that block de novo purinenucleotide synthesis. After methyl methane sulfonate (MMS) exposure, signifcant increases of 6-TG resistant colony were observed.
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