1998 Fiscal Year Final Research Report Summary
Pathological study of adnesion molecoles on multinvcleation of ostooclasts in GCT
Project/Area Number |
08670194
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Human pathology
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Research Institution | Okinaka Memorial Institute for Medical Research (1998) The University of Tokyo (1996-1997) |
Principal Investigator |
HORIUCHI Hajime Okinaka Memorial Institute for Medical Research, 研究員 (10190240)
|
Co-Investigator(Kenkyū-buntansha) |
KASHIWA Takeshi Tokyo University, Department of Pathology, Assistant, 大学院・医学系研究科, 助手 (70292729)
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Project Period (FY) |
1996 – 1998
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Keywords | GCT / adhesion molecde / osteoclast / immunohisto chemistry / monoclonal antibody |
Research Abstract |
Giant cell tumor of bone (GCT) is a kind of benign bone tumor which shows a unique histologic feature, that is the tumor is composed of mononuclear stromal cells and multinucleated osteoclast-like giant cells.In this study, we investigated expression of adhesion molecules to clarify that giant cells might be developed from stromal cells by fusion and raised monoclonal antibodies to GCT to determine the phenotypic relationship between GCT and osteoclasts. We studies expression of E-cadherin, OB-cadherin and CD44 in GCT.RT-PCR method revealed expression of CD44H m-RNA in all GCT cases.In immunohistochemistry, stromal cells and giant cells expressed CD44H.OB-cadherin m-RNA expression was observed in surgically removed GCT tissues, and showed normal form predominance.Expression of E-cadherin wasn't detected.CD44H is a marker of osteoclastic phenotype and that is expressed in stromal and giant cells of GCT, which means both cells of GCT showed osteoclastic differentiation.Furthermore, CD44 was reported to play a role in multinucleation of osteoclasts and it might be also in case in GCT. We raised two monoclonal antibodies to GCT and designated 1D8 and 3C8.Both antibodies reacted with stromal and giant cells of GCT.In normal tissues, only osteoclasts reacted with these two antibodies simultaneously.These results suggested stromal cells and giant cells of GCT belongs to osteoclastic lineage.
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