1997 Fiscal Year Final Research Report Summary
The study of intrathymic islet transplantation.
| Project/Area Number |
08671334
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Chiba University |
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Principal Investigator |
KENMOCHI Takashi Department of Surgery (II), Chiba University, School of Medicine, Assistant, 医学部・付属病院, 助手 (50215133)
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| Co-Investigator(Kenkyū-buntansha) |
ISONO Kaichi Department of Surgery (II), Chiba University, School of Medicine, Professor, 医学部, 教授 (70009489)
NAKAGOHRI Toshio Department of Surgery (II), Chiba University, School of Medicine, Assistant, 医学部, 助手 (10261918)
NAKAJIMA Kazuaki Department of Surgery (II), Chiba University, School of Medicine, Assistant, 医学部, 助手 (20261919)
ASANO Takehide Department of Surgery (II), Chiba University, School of Medicine, Lecturer, 医学部, 講師 (80143311)
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| Project Period (FY) |
1996 – 1997
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| Keywords | Islet transplantation / Intrathymic transplantation / Anti lymphocyte gloublin / Gene transfection / Major histocompatibility complex |
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| Research Abstract |
For the purpose of immunosuppression after pancreatic islet alloranft, the following three projects were studied ; 1.Efficacy of intrathymic islet alloranft, 2.Gene transfection into isolated islets, 3.The role of Major histocompatibility complex in porcine islet allograft. 1.Using rat islet allograft model, the islets transplanted into the thymus survived more than 100 days with a single antilymphocyte gloublin. In contrast, the islets transplanted beneith the kidney capsel survived 31.5 days (median survival). Furthermore, intrathymic islet allograft induced the donor-specific torelance evaluated by in vitro assay (MLR). 2.The islets isolated from Rat pancreata were transfected with pZeoSV2/lacZ Vector using a liposomal method Transfer rates evaluated by X-gal staining showed over 90% with good endocrine function assayd by static incubation. 3.Using a MHC (SLA) matched NIH miniature pigs, the islet allograft without immunosuppression was perfomed. The recipient pigs showed normoglycemia more than two weeks. This result suggested the important role of MHC on islet allograft. These results demonstrated that various approach might realize the islet allograft wothout continuous immnosuppression.
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