Fundamental study of endothelial cell growth and collagen production on functional protein chemically fixed vascular prosthesis

1997 Fiscal Year Final Research Report Summary

• Project/Area Number: 08671539
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Rakuno Gakuen University
• Principal Investigator: IZUMISAWA Yasuharu Rakuno Gakuen Univ., School of Vet. Med. Associate Professor, 獣医学部, 助教授 (60193380)
• Co-Investigator(Kenkyū-buntansha): SAKUMA Makoto Hokkaido Univ., School of Medicine Associate Professor, 医学部, 助教授 (70170636) ; YAMASHITA Kazuto Rakuno Gakuen Univ., School of Vet. Med. Assistant Professor, 獣医学部, 講師 (60244844) ; TANIYAMA Hiroyuki Rakuno Gakuen Univ., School of Vet. Med. Associate Professor, 獣医学部, 助教授 (90133800)
• Project Period (FY): 1996 – 1997
• Keywords: functional protein / chemical fixing / small caliber vascular prosthesis / endothelialcell / canine / common carotid artery / collagen

Research Abstract

Canine cultured endothelial cells were seeded on ePTFE that were chemically fixed with ten kinds of functional protein (BPE/MA,TGF-alpha/MA,TGF-alpha/FN/MA,apo-transferrin/MA,apo-transferrin/MA,Fe-citrate/MA,Fe-citrate/FN/MA,insulin/MA,insulin/FN/MA,transferrin/MA), and their growth was evaluated. Endothelial cells grewwell on Insulin/MA/ePTFE and transferrin/MA/ePTFE,with high total cell counts and high number of colony-forming cells.
On the basis of this result, end-to-end anastomoses of apo-transferrin/MA/ePTFE,apo-transferrin/FN/MA/ePTFE,insulin/MA/ePTFE,and insulin/FN/MA were performed on both common carotid arteries of 8 dogs. The sizes of the ePTFE were 3-4mm in internal diameter and 10mm in length. The patency of the grafts within two weeks was examined by using a doppler ultrasound blood flow meter, B-mode imaging ultrasonography and angiography. Histologically, implanted ePTFE were evaluated by light microscopy with HA staining and immuno-histochemical staining of collagen type I,III and alpha-smooth muscle actin. Fifty % of apo-transferrin/FN/MA/ePTFE and insulin/FN/MA/ePTFE were patent, and early endothelialization and vascularization were confirmed at one week after surgery.
From these results, it was concluded that early endothelialization is an important factor for patency of small-caliber vascular grafts such as ePTFE and that apo-transferrin/FN/MA or insulin/FN/MA chemically fixed ePTFE,which possess a higher endothelial cell proliferation, are useful.