1997 Fiscal Year Final Research Report Summary
Diagnosis of malaria by RT-PCR
| Project/Area Number |
08672633
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | The University of Tokyo |
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Principal Investigator |
FURUTA Takahisa The University of Tokyo, Institute of Medical Science, Research associate, 医科学研究所, 教務職員 (30143514)
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| Co-Investigator(Kenkyū-buntansha) |
KIMURA Mikio The University of Tokyo, Institute of Medical Science, Research associate, 医科学研究所, 助手 (90114462)
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| Project Period (FY) |
1996 – 1997
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| Keywords | malaria / RT-PCR / Diagnosis |
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| Research Abstract |
We describe here a reverse transcriptase-polymerase chain method for the detection of malaria parasites. Ten in vitro-cultured isolates of Plasmodium falciparum and 16 specimens from patients infected with P.falciparum were used to examine the specificity and sensitivity of the test. The sensitivity of the test was 0.3 parasites per microliter of blood. Specificity was determined by matching the sequences of the specimens' DNA to published sequences of 18S ribosomal RNA gene in the species-specific region. the test proved to be very sensitive and specific for the detection of P.falciparum infection.
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