1997 Fiscal Year Final Research Report Summary
Studies on the interaction between cell adhesion molecules and the membrane skeleton in the nervous system
| Project/Area Number |
08680875
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neuroscience in general
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| Research Institution | National Institute For Environmental Studies |
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Principal Investigator |
KUNIMOTO Manabu National Institute for Environmental Studies, Environmental Health Sciences Division, Senior Researcher, 環境健康部, 主任研究 (20142101)
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| Project Period (FY) |
1996 – 1997
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| Keywords | Cell adhesion molecule / Nervous system / Membrane skeleton / Brain ankyrin / Development |
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| Research Abstract |
Ankyrins are a family of spectrin-binding proteins that link the spectrin/actin network to cytoplasmic domains of integral membrane proteins. AnkyrinB,the major ankyrin in the brain includes at least two isoforms of 220 kD,the major ankyrin isoform in adult rat brain and 440 kD, which is maximally expressed in developing neonatal rat brain and is most abundant in regions comprised of unmyelinated axons. In this study, I examined the expression and localization of the ankyrinB isoforms in developing nervous system of prenatal rats and compared with those of other neuronal proteins, including cell adhesion molecule L1 known as an ankyrin-binding protein. 440-kD ankyrinB appeared as early as on embryonic day 12 and increased progressively towards the day of birth, which was similar to the expression pattern of L1, GAP-43 and tau. On the other hand, 220-kD ankyrinB was expressed at a low level but constitutively throughout the latter prenatal period and was even a major isoform before embryonic day 14. While the localization of 440-kD ankyrinB was essentially confined to the axons, judging from the colocalization with GAP-43 and tau, 220-kD ankyrinB showed rather general distribution. The localization of L1 was similar to that of 440-kD ankyrinB,suggesting their specific interaction.
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