2000 Fiscal Year Final Research Report Summary
Ecological surveys and establishment of propagation techniques of Cypripedium plants in Japan, North America and Western Europe.
| Project/Area Number |
09041141
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| Research Category |
Grant-in-Aid for Scientific Research (A).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
生態
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| Research Institution | Tochigi Junior College, Kokugakuin University (1999-2000)
Hokkaido University of Education (1997-1998) |
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Principal Investigator |
TANIGUCHI Hirokazu Tochigi Junior College, Kokugakuin University, Professor, 初等教育学科, 教授 (60002771)
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| Co-Investigator(Kenkyū-buntansha) |
KATSUMI Masayuki Division of Natural Sciences, International Christian University, Professor, 名誉教授 (10052242)
SANO Hiroshi Research and Education Center for Genetic Information, Nara Institute of Science and Technology, Professor, 教授 (20178809)
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| Project Period (FY) |
1997 – 2000
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| Keywords | The genus Cypripedium / DNA analysis / in vitro seed germination / C.macranthos L.var.rebunenese / C.calceolus |
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| Research Abstract |
In this study, we have made(1)comparative ecological surveys of Cypripedium plants growing in North America, Western European, Fareast Russia and Japan, (2) comparative DNA analysis on these plants to understand their phylogetetic relationships and surveys of current situations of in vitro propagation techniques developed in foreign countries. We have also tried in vitro germination of C.macranthos L.cv.rebunenese(4).
The area where we made surveys in the past three years are Canadian Rocky Mountains(2 times), Prince Edward Island and halifax(once), the vicinities of Oregon and the Five Lakes District in USA(7 times), Switzerland and England(2 times)and Nadhodka(once). Leaf samples collected in these surveys were subjected to chloroplast DNA analysis. The results suggest that the geological rout of evolution of C.calceolus is likely from Fareast to North America via Japan. An interesting finding is that C.calceolus plants found in Rebun Island and Kitamai, Hokkaido belongs to the group on the basis the present DNA analysis. They are clearly different from those in Europe and China, suggesting that those found in Rebun Island and kitami are not denizens from other countries. In vitro propagation techniques of Cypripedium in general are probably most advanced in North America. We also have succeeded in seed germination and seedling formation of C.macranthos L.var.rebunenese and C.calceolus. However, it will take a considerably long time to induce flowering.
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[Publications] Taniguchi, H., Nakamura, T., Mizukami, H., Kawano, S., Sano, H.and Katsumi, M.: "Identity of Cypripedium calceolus(Orchidacese)in Rebun Island-Comparative DNA analysis on the related species."Genes Genet.Syst.. 76. 181-188 (2001)
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