1998 Fiscal Year Annual Research Report

哺乳類の受精における卵と精子の相互作用に関する研究

Research Project

Project/Area Number	09044240
Research Institution	Okazaki National Research Institutes
Principal Investigator	毛利秀雄岡崎国立共同研究機構, 基礎生物学研究所, 所長 (70012268)
Co-Investigator(Kenkyū-buntansha)	STEWART・SAVA ジェイニューオリンズ大学, 生物科学, 副教授 SUAREZ S.S. コーネル大学, 獣医学部, 副教授 YANAGIMACHI アールハワイ大学, 医学部, 教授石島純夫東京工業大学, 理学部, 助手 (70193315) 奥野誠東京大学, 総合文化研究科, 助教授 (40143325)
Keywords	哺乳類精子 / 超活性化 / 受精能獲得 / 鞭毛運動 / 先体反応 / 卵・精子相互作用
Research Abstract	今年度は以下のような成果が得られた。 1)ハムスターとサルの精子を用いて、超活性化と先体反応の鞭毛運動に対する影響を調べた。受精能獲得用溶液で培養3時間でほとんどのハムスター精子は鞭毛屈曲の非対称性のため頭を大きく振る円運動を示し、更に1時間後先体反応がおこると、中片部の異常な屈曲も加わり前進しなくなることが分かった。サルでも同様であった。2)ハムスター精子がグルコースにより一時的にその運動が阻害される現象につき解析がなされた。エネルギー代謝系に何らかの作用をしていると考えられる。また運動活性化の際にタンパク質のリン酸化だけでなく、脱リン酸化される低分子ダンパク質の関与が示唆された。3)ウシ精子と軸卵管上皮苧の結合が先体の細胞膜にあるレクチン様の分子を介しておこることを明らかにした。この分子はフコースを認識する。精子が受精能獲得すると、フコースへの結合能が失われ、精子は上皮から離れる。4)精子の先体部分に緑色蛍光タンパク質を発現させて、先体反応を非侵襲的に観察できる系が確立された。今後の解析に用いる予定である。5)t-ハロタイプ・マウスは非メンデル型遺伝をしてもハロタイプが集団に残るが、この現象に関与するTctex遺伝子の2つが、精子鞭毛運動のモーターであるダイニンの2本の軽鎖に相当することが明らかにされた。6)カナダのモントリオールで第8回国際精子シンポジウムが行われた機会を捉えて、ほとんどの班員が集まり、現況報告と今後の方針についての検討を行った。

Research Products
(22 results)

All Other

All Publications (22 results)

[Publications] Kubo-Irie,M.et al.(Mohri,H.): "Morphological evidence that spermatogenic cells undergo apoptosis within testis of diapausing swallowtail pupa (Atrophaneura alcinous)" Journal of Experimental Zoology. 283. 194-201 (1999)
[Publications] Kubo-Irie,M.et al.(Mohri,H.): "Morphological changes in eupyrene and apyrene spermatozoa in the reproductive tract of the male butterfly Atrophaneura alcinous Klug" Invertebrate Reproduction and Development. 34. 259-268 (1998)
[Publications] Si,Y.and Okuno,M.: "Rebulation of microtubule sliding by a 36-kDa phosphoprotein in Hamster sperm flagella" Molecular Reproduction and Development. 52. 328-334 (1999)
[Publications] Ishijima,S., Hara,M.and Okiyama M.: "Comparative studies on the spermatozoan motility of Japanese fshes." Bull.Ocean Res.Inst.Univ.Tokyo. 33. 139-152 (1998)
[Publications] Hadjantonakis,A. et al.(Okaze,M.): "Non-invasive sexing of preimplantation stage mammalian embryos." Nat Genet. 19. 220-222 (1998)
[Publications] Ikawa,M. et al.(Okabe,M.): "Green mice' and their potential usage in biological research." FEBS Lett. 430. 83-87 (1998)
[Publications] Tsujimura,A. et al.(Okabe,M.): "Molecular cloning of a murine homologue of membarane cofactor protein (CD46) : preferential expression in testicular germ cells." Biochem.J.330. 163-168 (1998)
[Publications] Yamagata,K. et al.(Okabe,M.): "Acrosin accelerates the dispersal of sperm acrosomal proteins during acrosome reaction." J.Biol.Chem.273. 10470-10474 (1998)
[Publications] Kagami,O. et al.(Ogawa,K.): "A dynein light chain of sea urchin sperm flagella is a homolog of mouse T ctex L which is encoded by a gene of the t complex sterility locus" Gene. 211. 383-386 (1998)
[Publications] Suarez,S.S. et al.: "Bull sperm binding to oviductal epithelium is mediated by a Ca2+- dependent lectin on sperm that recognizes Lewis-a grisaccharide." Biol.Reprod.59. 39-44 (1998)
[Publications] Suarez,S.S. et al.: "Minireview. The Oviductal sperm reservoir in mammals : Mechanisms of Formation." Biol.Reprod.58. 1105-1107 (1998)
[Publications] Ho,H.C., Suarez,S.S.: "Three-dimensional structure of the Golgi complex in mouse spermatids : a scanning electron microscope study." Proceedings of the International Meeting of Spermatology, Montreal, Canada, August 1998. Abs.2-43 (1998)
[Publications] Imai,E. et al.(Okabe,M.): "Glowing podocytes in living mouse : transgenic mouse carrying a podocyte-specific promoter (In Process Citation)" Exp Nephorol. 7. 63-66 (1999)
[Publications] Koga,M. et al.(Okabe,M.): "Expression of selenoprotein-P messenger ribonucleic acid in the rat testis." Biol.Rep.58. 261-265 (1998)
[Publications] Matsuki,M. et al.(Okabe,M.): "Defective stratum corneum and early neonatal death in mice lacking the gene for transglutaminase 1 (keratinocyte transglutaminase)" Proc Natl Acad Sci USA. 95. 1044-1049 (1998)
[Publications] Mikata,S. et al.(Okabe,M.): "Revention of hyperacute rejection by phosphatidylinositol-anchored mini-complement receptor type 1." Transpl Immunol. 6. 107-110 (1998)
[Publications] Okaze,M. et al.: "Green fluorescent protein and its usage in reproductive biology." Zygote. 6. 117-117 (1998)
[Publications] Takahama,Y. et al.(Okabe,M.): "Functional competence of T cells in the absence glycosylphosphatidylinositol-anchored proteins caused by T cell-specific disruption of the Pig-a gene." Eur.J.Immunol.28. 2159-2166 (1998)
[Publications] Revah I, et al.(Suarez,S.S.): "Changes in capacity of bull sperm to bind fucose." Biol Repro 58 (Suppl 1). Abs.147-147 (1998)
[Publications] Maleszewski,M. et al.(Yanagimachi,R.): "Behavior of transgenic mouse spermatozoa with galline protamine." Biol.Reprod.58. 8-14 (1998)
[Publications] Stewart-Savage,J. et al.: "Developmental basis of phenotypic variation in egg production in a colonial ascidian : primary oocyte production versus oocyte development." Biol.Bull.196. 63-69 (1999)
[Publications] Stewart-Savage,J. et al.: "Suppression of motility in actively moving hamster sperm by hexose." Eighth International Symposium on Spermatology, August 1998, Montreal, Canada. (Poster presentation). (1998)

1998 Fiscal Year Annual Research Report

哺乳類の受精における卵と精子の相互作用に関する研究

Principal Investigator

毛利 秀雄 岡崎国立共同研究機構, 基礎生物学研究所, 所長 (70012268)

Research Products

[Publications] Kubo-Irie,M.et al.(Mohri,H.): "Morphological evidence that spermatogenic cells undergo apoptosis within testis of diapausing swallowtail pupa (Atrophaneura alcinous)" Journal of Experimental Zoology. 283. 194-201 (1999)

[Publications] Kubo-Irie,M.et al.(Mohri,H.): "Morphological changes in eupyrene and apyrene spermatozoa in the reproductive tract of the male butterfly Atrophaneura alcinous Klug" Invertebrate Reproduction and Development. 34. 259-268 (1998)

[Publications] Si,Y.and Okuno,M.: "Rebulation of microtubule sliding by a 36-kDa phosphoprotein in Hamster sperm flagella" Molecular Reproduction and Development. 52. 328-334 (1999)

[Publications] Ishijima,S., Hara,M.and Okiyama M.: "Comparative studies on the spermatozoan motility of Japanese fshes." Bull.Ocean Res.Inst.Univ.Tokyo. 33. 139-152 (1998)

[Publications] Hadjantonakis,A. et al.(Okaze,M.): "Non-invasive sexing of preimplantation stage mammalian embryos." Nat Genet. 19. 220-222 (1998)

[Publications] Ikawa,M. et al.(Okabe,M.): "Green mice' and their potential usage in biological research." FEBS Lett. 430. 83-87 (1998)

[Publications] Tsujimura,A. et al.(Okabe,M.): "Molecular cloning of a murine homologue of membarane cofactor protein (CD46) : preferential expression in testicular germ cells." Biochem.J.330. 163-168 (1998)

[Publications] Yamagata,K. et al.(Okabe,M.): "Acrosin accelerates the dispersal of sperm acrosomal proteins during acrosome reaction." J.Biol.Chem.273. 10470-10474 (1998)

[Publications] Kagami,O. et al.(Ogawa,K.): "A dynein light chain of sea urchin sperm flagella is a homolog of mouse T ctex L which is encoded by a gene of the t complex sterility locus" Gene. 211. 383-386 (1998)

[Publications] Suarez,S.S. et al.: "Bull sperm binding to oviductal epithelium is mediated by a Ca2+- dependent lectin on sperm that recognizes Lewis-a grisaccharide." Biol.Reprod.59. 39-44 (1998)

[Publications] Suarez,S.S. et al.: "Minireview. The Oviductal sperm reservoir in mammals : Mechanisms of Formation." Biol.Reprod.58. 1105-1107 (1998)

[Publications] Ho,H.C., Suarez,S.S.: "Three-dimensional structure of the Golgi complex in mouse spermatids : a scanning electron microscope study." Proceedings of the International Meeting of Spermatology, Montreal, Canada, August 1998. Abs.2-43 (1998)

[Publications] Imai,E. et al.(Okabe,M.): "Glowing podocytes in living mouse : transgenic mouse carrying a podocyte-specific promoter (In Process Citation)" Exp Nephorol. 7. 63-66 (1999)

[Publications] Koga,M. et al.(Okabe,M.): "Expression of selenoprotein-P messenger ribonucleic acid in the rat testis." Biol.Rep.58. 261-265 (1998)

[Publications] Matsuki,M. et al.(Okabe,M.): "Defective stratum corneum and early neonatal death in mice lacking the gene for transglutaminase 1 (keratinocyte transglutaminase)" Proc Natl Acad Sci USA. 95. 1044-1049 (1998)

[Publications] Mikata,S. et al.(Okabe,M.): "Revention of hyperacute rejection by phosphatidylinositol-anchored mini-complement receptor type 1." Transpl Immunol. 6. 107-110 (1998)

[Publications] Okaze,M. et al.: "Green fluorescent protein and its usage in reproductive biology." Zygote. 6. 117-117 (1998)

[Publications] Takahama,Y. et al.(Okabe,M.): "Functional competence of T cells in the absence glycosylphosphatidylinositol-anchored proteins caused by T cell-specific disruption of the Pig-a gene." Eur.J.Immunol.28. 2159-2166 (1998)

[Publications] Revah I, et al.(Suarez,S.S.): "Changes in capacity of bull sperm to bind fucose." Biol Repro 58 (Suppl 1). Abs.147-147 (1998)

[Publications] Maleszewski,M. et al.(Yanagimachi,R.): "Behavior of transgenic mouse spermatozoa with galline protamine." Biol.Reprod.58. 8-14 (1998)

[Publications] Stewart-Savage,J. et al.: "Developmental basis of phenotypic variation in egg production in a colonial ascidian : primary oocyte production versus oocyte development." Biol.Bull.196. 63-69 (1999)

[Publications] Stewart-Savage,J. et al.: "Suppression of motility in actively moving hamster sperm by hexose." Eighth International Symposium on Spermatology, August 1998, Montreal, Canada. (Poster presentation). (1998)

毛利秀雄岡崎国立共同研究機構, 基礎生物学研究所, 所長 (70012268)