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1998 Fiscal Year Final Research Report Summary

Isolation and transformation of genes from woody plants that have high ability to clean up air polllutants

Research Project

Project/Area Number 09355028
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 生物・生体工学
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

MORIKAWA Hiromichi  Fac.of Science, HIROSHIMA UNIVERSITY Professor, 理学部, 教授 (00089129)

Co-Investigator(Kenkyū-buntansha) GOSHIMA Naoki  Fac.of Science, Associate Professor, 理学部, 助教授 (70215482)
TAKAHASHI Misa  Fac.of Science, Research Associate, 理学部, 助手 (10294513)
OOSAWA Shigeyoshi  Green Tech Co., Executive Researcher, 取締役研究部長
Project Period (FY) 1997 – 1998
KeywordsNitrogen dioxide / Air Pollution / Remediation of Environment / Nitrate assimilation / Rhododedron / Ficus thunbergii / Woody plants / 2-D gel electrophoresis of proteins
Research Abstract

This project is aimed at the isolation of genes from woody plants such as Rhododendron mucronatum that have high ability to clean up nitrogen dioxide, a typical air pollutant. Using 2-D gel electrophoresis of proteins, we have found that polypeptide spots of p1 5.6 and MW 25-26kD increased their intensities by four to five times in response to fumigation with nitrogen dioxide. About 20 amino acid sequences at the amino terminals of these polypeptides were determined by vapor phase method. Homology search indicated that these polypeptide had 57-68% identity with so-called gemin from Arabidopsis thaliana. Cloning of the full length of cDNA that encodes this polypeptide was investigated. Using differential display method, we have found that several mRNA from the leaves Rhododendron mucronatum increased or decreased their intensities in response to fumigation with nitrogen dioxide. The DNA sequences of several bands were determined, and homology search indicated these DNAs did not have any distinct homology with reported proteins or cDNAs, suggesting that some of them are novel genes whose expression is induced or decreased upon fumigation with nitrogen dioxide. Transformation of Arabidopsis thaliana and Ficus thunbergii using particle bombardment method has also been investigated.

  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] H.Inada: "Existence of three regulatory regions each containing a highly conserved motif in the promoter of plastid-encoded RNA polymerase gene (rpo B)." Plant Journal. 11・4. 883-890 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Seki: "Transient expression of foreign genes in tissues of Arabidopsis thaliana by bombardment-mediated transformation" Methods in Molecular Biology. 82・4. 219-225 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Morikawa: "More than a 600-fold variation in nitrogen dioxide assimilation among 217 plant taxa" Plant,Cell & Environment. 21・2. 180-190 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Takahashi: "Stable transformation of Eustoma grandiflorum by particle bombardment" Plant Cell Reports. 17・4. 504-507 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] G.Arimura: "Expression of the β-glucuronidase gene introduced into intact leaves attached to Arabidopsis thaliana plants by particle gun" Plant Biotechnology. 15・1. 109-111 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Sawasaki: "The structure of transgene loci in transgenic Arabidopsis plants obtained by particle bombardment:Junction regions have the ability to bind to nuclear matrices." Gene. 218・1. 27-35 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] G.Arimura: "Unidentified nitrogen in the metabolites of nitrogen dioxide in plant leaves" Proceedings of the XIth International Congress on Photosynthesis,Kluwer Academic Publishers. in press. (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Takahashi: "Is nitrite reductase essential in the metabolism of nitrogen in plants?" Proceedings of the XIth International Congress on Photosynthesis,Kluwer Academic Publishers. in press. (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Morikawa: "In "Pollen biotechnology for crop production and improvement" eds.By R.K.Sawhney and K.V.Shivanna,Cambridge University Press,pp.423-437 (1997)." Use of pollen in gene transfer, 448 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Morikawa: "In "Stress Responses of Photosynthetic Organisms",ed.by K.Satoh and N.Murata,Elsevier,pp.227-237(1997)." Molecular mechanism of the metabolism of nitrogen dioxide as an alternative fertilizer in plants., 260 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Morikawa: "Air Pollution and Biotechnology in Plants ed.by K.Oomasa et al." Springer-Verlag, 256 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Inada, M.Seki, H.Morikawa, M.Nishimura and K.Iba: "Existence of three regulatory regions each containing a highly conserved motif in the promoter of plastid-encoded RNA polymerase gene (rpo B)" Plant Journal. 11(4). 883-890 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Seki, A.Iida and H.Morikawa: "Transient expression of foreign genes in tissues of Arabidopsis thaliana by bombardment-mediated transformation" Methods in Molecular Biology. 82(4). 219-225 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Morikawa, A.Higaki, M.Nohno, M.Takahashi, M.Kamada, M.Nakata, G.Toyohara, Y.Okamura, K.Matsui, S.Kitani, K.Fujita, K.Irifune, and N.Goshima: "More than a 600-fold variation in nitrogen dioxide assimilation among 217 plant taxa" Plant Cell & Environment. 21(2). 180-190 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Takahashim, M.Nishihara, S.Yamamura, S.Nishizawa, K.Irifune and H.Morikawa: "Stable transformation of Eustoma grandiflorum by particle bombardment." Plant Cell Reports. 17(4). 504-507 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] G.Arimura, H.Banjo, K.Irifune, K.-H.Pwee, M.Takahashi and H.Morikawa: "Expression of the beta-glucuronidase gene introduced into intact leaves attached to Arabidopsis thaliana plants by particle gun" Plant Biotechnology. 15(1). 109-111 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Sawasaki, M.Takahashi, N.Goshima and H.Morikawa : The structure of transgene loci in transgenic Arabidopsis plants obtained by particle bombardment: "Junction regions have the ability to bind to nuclear matrices" Gene. 218(1). 27-35 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] G.Arimura, M.Takahashi, N.Goshima and H.Morikawa: "Unidentified nitrogen in the metabolites of nitrogen dioxide in plant leaves" Proceedings of the XIth International Congress on Photosynthesis, Kluwer Academic Publishers. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Takahashi, M.Caboche and H.Morikawa: "Is nitrite rerductase essential in the metabolism of nitrogen in plants?" Proceedings of the XIth International Congress on Photosynthesis, Kluwer Academic Publishers. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Morikawa and M.Nishihara: "Use of pollen in gene transfer." In "Pollen biotechnology for crop production and improvement" eds.by R.K.Sawhney and K.V.Shivanna, Cambridge University Press. 423-437 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Morikawa, M.Takahashi and K.Irifune: "Molecular mechanism of the metabolism of nitrogen dioxide as an alternative fertilizer in plants." In "Stress Responses of Photosynthetic Organisms", ed.by K.Satoh and N.Murata, Elsevier. 227-237 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Morikawa, M.Takahashi and G.Arimura: "Manipulation of genes for nitrogen metabolism in plants." In Air Pollution and Biotechnology in Plants ed.by K.Oomasa et al., Springer-Verlag. (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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