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2000 Fiscal Year Final Research Report Summary

Developments in the new glycotechnology using the specific activity of endo-glycosidases

Research Project

Project/Area Number 09358013
Research Category

Grant-in-Aid for Scientific Research (A).

Allocation TypeSingle-year Grants
Section展開研究
Research Field Structural biochemistry
Research InstitutionHirosaki University

Principal Investigator

ENDO Masahiko  Hirosaki University, Biochemistry, Professor, 医学部, 教授 (20004616)

Co-Investigator(Kenkyū-buntansha) ITO Makoto  Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Bioscience and Biotechnology, Associate Professor, 大学院・生物資源環境科学研究科, 助教授 (40253512)
YAMAMOTO Kenji  Graduate School of Biostudies, Kyoto University Integrant Life Science, Professor, 大学院・農学研究科, 教授 (70109049)
HASE Sumihiro  Graduate School of Science, Osaka University, Chemistry, Professor, 大学院・理学研究科, 教授 (80028232)
ITO Kazuo  Osaka City University, Biology of Science, Associate Professor, 理学部, 講師 (20183171)
TAKEGAWA Kaoru  Kagawa University, Life Science, Associate Professor, 農学部, 助教授 (50197282)
Project Period (FY) 1997 – 2000
Keywordsendo-type glycosidase / transglycosylation / carbohydrate transferation in to peptide / cutting out carbohydrate chain / glycoconjugate / linkage region / glycotechnology
Research Abstract

The objective
This research group is composed of researchers who have each independently discovered some new endo-type glycosidases. It is extremely important that these enzymes act on the linkage region between a core molecule (protein or lipid) and a carbohydrate chain of glycoconjugates, and as a result release a long carbohydrate chain from a core molecule. The enzymes are endo-β-N-acetylglucosaminidase and endo-β-mannosidase acting on N-glycan, endo-α-N-acetylgalactosaminidase acting on O-glycan, endo-β-xylosidase, endo-β-galactosidase and endo-β-glucuronidase acting on proteoglycan, and endo-glucoceramidase acting on glycolipid. Therefore, the object of the research is to open a new avenue for the glycotechnology using the specific properties of the enzyme, that is the reconstruction of carbohydrate chains and the transferation of carbohydrate chaing into peptide or lipid.
The fundamental research policy
The fundamental research policy is as follows :
1. To establish the purification methods for and elucidate the properties of the enzymes.
2. To establish the effective methods for cutting out the carbohydrate chains from glycoconjugate.
3. To establish the transglycosylation and reconstruction methods for carbohydrate chains for glycoconjugate.
The results
1. Purification methods for each enzyme were established and the properties of each enzyme were elucidated. Moreover, the cloning of some of the enzymes is in progress.
2. Methods of cutting out the carbohydrate chains from glycoconjutate were established for each enzyme.
3. The conditions for the transferation and reconstruction of each enzyme were estimated as a reverse reaction of hydrolysis reaction of endo-type glycosidases. However, as the conditions for each of those reactions were different, the establishment of effective conditions is an important remaining problem.

  • Research Products

    (69 results)

All Other

All Publications (69 results)

  • [Publications] Izu,Y.et al.: "Molecular cloning, expression, and sequence analysis of the endoglycoceramidase II gene from Rhodococcus sp. strain M-777."J.Biol.Chem.. 272. 19846-19850 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishii-Karakasa, I. et al.: "Structural determination of the O-linked sialyl oligosaccharides liberated from fetuin with endo-α-N-acetyl galactosaminidase-S by HPLC analysis and 600-MHz ^1H-NMR spectroscopy."Eur.J.Biochem.. 247. 661-664 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamamoto,K. et al.: "Chemo-enzymatic synthesis of a novel glycopeptide using a microbial endoglycosidase."Carbohyd.Res.. 305. 415-422 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Haneda,K. et al.: "Chemo-enzymatic synthesis of calcitonin derivatives containing N-Linked oligosaccharides."Bioorg.Med.Chem.Lett.. 8. 1303-1306 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okino,N. et al.: "Purification and characterization of a novel ceramidase from Pseudomonas aeruginosa."J.Biol.Chem.. 273. 14368-14373 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ji,L. et al.: "Effects of endoglycoceramidase or D-threo PDMP on glucose uptake, glycolysis and mitochondrial respiration in HL60 cells."Arch.Biochem.Biophys.. 359. 107-114 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takegawa,K. et al.: "Enzymatic synthesis of a neoglycoconjugate by transglycosylation with Arthrobacter endo-β-N-acetylglucosaminidase : a substrate for colorimetric detection of endo-β-N-acetylglucosaminidase Activity."Anal.Biochem.. 257. 218-223 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Deras,I.L. et al.: "Synthesis of a high-mannose-type oligosaccharide glycopeptide analog containing a glucose-asparagine linkage."Bioorg.Med.Chem.Lett.. 8. 1763-1766 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Anindyawati,T. et al.: "Three different types of a-amylases from Aspergillus aw amori KT-11 : Their purifications, properties, and specificities."Biosci.Biotechnol.Biochem.. 62. 1351-1357 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tanaka,Y. et al.: "Screening and fermentation of endo-α-N-acetylgalactosaminidase S, a mucin-hydrolyzing enzyme from Streptomyces acting on the GalNAc-O-Ser(Thr) Linkage."Journal of Fermentation and Bioengineering.. 85. 381-387 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mizuno,M. et al.: "Synthesis of a glycopeptide containing oligosaccharides."J.Am.Chem.Soc.. 121. 284-290 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Komori,H. et al.: "Regulation of intracellular ceramide content in B 16 melanoma cells Biological implications of ceramide glycosylation."J.Biol.Chem.. 274. 8981-8987 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sakaguchi,K. et al.: "The Glu residue in the conserved Asn-Glu-Pro sequence of endoglycoceramidase is essential for enzymatic activity."Biochem.Biophys.Res.Commun.. 260. 89-93 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miura,Y. et al.: "Requirement for a different hydrophobic moiety and a reliable chromogenic substrate for endo-type glycosylceramidases."Glycobiology.. 9. 957-960 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okino,N. et al.: "Molecular cloning, sequencing, and expression of the gene encoding alkaline ceramidase from Pseudomonas aeruginosa. -Cloning of a eramidase homologue from Mvcobacterium tuberculosis-"J.Biol.Chem.. 274. 36616-36622 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamamoto,K. et al.: "Chemo-enzymatic synthesis of a calcitonin derivative containing a high-mannose type oligosacchride by endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae."J.Biosci.Bioeng.. 87. 175-179 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Munakata H. et al.: "Interaction between collagens and glycosaminoglycans investigated using a surface plasmon resonance biosensor"Glycobiology.. 9. 1023-1027 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takagaki,K. et al.: "Enzymatic reconstruction of a hybrid glycosaminoglycan containing 6-sulfated, 4-sulfated, and unsulfated N-acetylgalactosamine."Biochem.Biophys.Res.Commun.. 258. 741-744 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Vejaratpimol,R. et al.: "Detection and serological relationships of cymbisium mosaic potexvirus isolates."J.Biosci.Bioeng.. 87. 161-168 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Makino,Y. et al.: "Structural analysis of N-linked sugar chains of human blood clotting factor IX."J.Biochem.. 128・2. 175-180 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Makino,Y. et al: "Processing pathway deduced from the structures of N-glycans in Carica papaya."J.Biochem.. 127・6. 1121-1126 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ashida,H. et al.: "Characterization of Endo-α-N-acetylgalactosaminidase from Bacillus sp.and Syntheses of Neo-oligosaccharides Using Its Transglycosylation Activity."Arch.Biochem.Biophys.. 373. 394-400 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tani,M. et al.: "Purification and characterization of a novel ceramidase from mouse liver."J.Biol.Chem.. 275. 3462-3468 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tani,M. et al.: "Molecular cloning of the full-length cDNA encoding mouse neutral ceramidase"J.Biol.Chem.. 275. 11229-11234 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Komori,H. et al.: "Regulation of UDP-glucose : ceramide glucosyltransferase-1 by ceramide."FEBS Letter.. 475. 247-250 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Horibata,Y. et al.: "Purification, characterization and cDNA cloning of a novel acidic endoglycoceramidase from the jellyfish, Cyanea nozakii."J.Biol.Chem.. 275. 31297-31304 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sakaguchi,K. et al: "Cloning and expression of gene encoding a novel endoglycoceramidase of Rhodococcus sp. strain C9. "J.Biochem.. 128. 145-152 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fujita,K. et al.: "Synthesis of nioglycoenzymes with homogeneous N-linked oligosaccharides using immobilized endo-β-N-acetylglucosaminidase A."Biochem.Biophys.Res.Commun.. 267. 134-138 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fujita,K. et al.: "A plate assay for endo-β-N-acetylglucosaminidase activity using a chromogenic substrate synthesized by transglycosylation with Arthrobacter endo-β-N-acetylglucosaminidase."J.Biosci.Bioeng.. 90. 462-464 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takagaki,K. et al.: "Enzymatic reconstruction of dermatan sulfate."Biochem.Biophys.Res.Commun.. 270. 588-593 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takagaki,K. et al.: "Chimeric glycosaminoglycan oligosaccharides synthesized by enzymatic reconstruction and their use in substrate specificity determination of Streptococcus hyaluronidase."J.Biochem.. 127. 695-702 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Otake,Y. et al.: "Analysis of N-linked oligosaccharides expressed in human sera from healthy controls and patients with non-small cell lung cancer."J.Biochem.,. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fujita,K. et al: "Identification of amino acid residues essential for the substrate specificity of Flavobacterium sp.endo-β-N-acetylglucosaminidase."Biosci.Biotechnol.Biochem.,. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ashida,H., et al.: "Enzymatic syntheses of T antigen-containing glycolipid mimicry using the transglycosylation activity of endo-α-N-acetylgalactosaminidase."Carbohyd.Res.,. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Haneda,K. et al.: "Chemo-enzymatic synthesis of a bioactive peptide containing a glutamine-linked oligosaccharide and its characterization."Biochem.Biophys.Acta.,. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishii-Karakasa,I. et al.: "Convenient method for structural determination of glycans related to diseases."New Develoment in Glycomedicine,. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishii-Karakasa,I. et al.: "Convenient method for full-assignment of ^1H-NMR spectrum of oligosaccharides."Bunseki Kagaku,. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ajisaka,K. et al.: "Efficient synthesis of O-linked glycopeptide by a transglycosylation using endo α-N-acetylgalactosaminidase from Streptomyces sp."Biosci.Biotechnol.Biochem.,. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Izu, Y., Izumi, Y., Kurome, Y., Sano, M., Kondo, A., Kato, I., and Ito, M.: "Molecular cloning, expression, and sequence analysis of the endoglycoceramidase II gene from Rhodococcus sp.strain M-777."J.Biol.Chem.. 272. 19846-19850 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishii-Karakasa, I., Iwase., I., and Hotta, K.: "Structural determination of the O-linked sialyl oligosaccharides liberated from fetuin with endo-α-N-acetylgalactosaminidase-S by HPLC analysis and 600-MHz ^1H-NMR spectroscopy."Eur.J.Biochem.. 247. 709-715 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamamoto, K., Fujimori, K., Haneda, K., Mizuno, M., Inazu, T., and Kumagai, H.: "Chemo-enzymatic synthesis of a novel glycopeptide using a microbial endoglycosidase."Carbohyd.Res.. 305. 415-422 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Haneda, K., Inazu, T., Mizuno, M., Iguchi, R., Yamamoto, K., Kumagai, H., Aimoto, S., Suzuki, H., and Noda, T.: "Chemo-enzymatic synthesis of calcitonin derivatives containing N-Linked oligosaccharides."Bioorg.Med.Chem.Lett.. 8. 1303-1306

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okino, N., Tani, T., Imayama, S., and Ito, M.: "Purification and characterization of a novel ceramidase from Pseudomonas aeruginosa."J.Biol.Chem.. 273. 14368-14373 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ji, L., Ito, M., Zhang, G., Hirabayashi, Y., Inokuchi, J., and Yamagata, T.: "Effects of endoglycoceramidase or D-threo PDMP on glucose uptake, glycolysis and mitochondrial respiration in HL60 cells."Arch.Biochem.Biophys.. 359. 107-114 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takegawa, K., Fujita, K., Fan, J.-Q., Tabuchi, M., Tanaka, N., Kondo, A., Iwamoto, H., Kato, I., Lee, Y,C., and Iwahara, S.: "Enzymatic synthesis of a neoglycoconjugate by transglycosylation with Arthrobacter endo-β-N-acetylglucosaminidase : a substrate for colorimetric detection of endo-β-N-acetylglucosaminidase Activity."Anal.Biochem.. 257. 218-223 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Deras, I.L., Takegawa, K., Kondo, A., Kato, I., and Lee, Y.C.: "Synthesis of a high-mannose-type oligosaccharide glycopeptide analog containing a glucose-asparagine linkage."Bioorg.Med.Chem.Lett.. 8. 1763-1766 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Anindyawati, T., Melliawati, R., Ito, K., Iizuka M.and Minamiura, N.: "Three different types of a-amylases from Aspergillus awamori KT-11 : Their purifications, properties, and specificities."Biosci.Biotechnol.Biochem.. 62. 1351-1357 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tanaka, Y., Takahashi, Y., Shinose, M., Omura, S., Ishii-Karakasa, I., Iwase, H., and Hotta, K.: "Screening and fermentation of endo-α-N-acetylgalactosaminidase S, a mucin-hydrolyzing enzyme from Streptomyces acting on the GalNAc-O-Ser (Thr) Linkage."Journal of Fermentation and Bioengineering. 85. 381-387 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mizuno, M., Haneda, H., Iguchi, R., Muramoto, I., Kawakami, T., Aimoto, S., Yamamoto, K., and Inazu, T.: "Synthesis of a glycopeptide containing oligosaccharides."J.Am.Chem.Soc.. 121. 284-290 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Komori, H., Ichikawa, S., Hirabayashi, Y., and Ito, M.: "Regulation of intracellular ceramide content in B16 melanoma cells Biological implications of ceramide glycosylation."J.Biol.Chem.. 274. 8981-8987 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sakaguchi, K., Okino, N., Izu, H., and Ito, M.: "The Glu residue in the conserved Asn-Glu-Pro sequence of endoglycoceramidase is essential for enzymatic activity."Biochem.Biophys.Res.Commun.. 260. 89-93 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miura, Y., Arai, T., Ito, M., Yamamoto, K., and Yamagata, T.: "Requirement for a different hydrophobic moiety and a reliable chromogenic substrate for endo-type glycosylceramidases."Glycobiology. 9. 957-960 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okino, N., Ichinose, S., Imayama, S., Nakamura, T., and Ito, M.: "Molecular cloning, sequencing, and expression of the gene encoding alkaline ceramidase from Pseudomonas aeruginosa. -Cloning of a eramidase homolo from Mycobacterium tuberculosis-"J.Biol.Chem.. 274. 36616-36622 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamamto, K., Haneda, K., Iguchi, R., Inazu, T., Mizuno, M., Takegawa, K., Kondo, A., and Kato, I.: "Chemo-enzymatic synthesis of a calcitonin derivative containing a high-mann type oligosacchride by endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae."J.Biosci.Bioeng.. 87. 175-179 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Munakata, H., Takagaki, K., Majima, M., and Endo.M.: "Interaction between collagens and glycosaminoglycans investigated using a surface plasmon resonance biosensor."Glycobiology. 9. 1023-1027 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takagaki, K., Munakata, H., Majima, M., and Endo.M.: "Enzymatic reconstruction of a hybrid glycosaminoglycan containing 6-sulfated, 4-sulfated, and unsulfated N-acetylgalactosamine."Biochem.Biophys.Res.Commun.. 258. 741-744 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Vejaratpimol, R., Channuntapipat, C., Pewnim, T., Ito, K., Iizuka, M.and Minamiura, N.: "Detection and serological relationships of cymbisium mosaic potexvirus isolates."J.Biosci.Bioeng.. 87. 161-168 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Makino, Y., Omichi, K., Kuraya, N., Ogawa, H., Nishimura, H., Iwanaga, S., and Hase, S.: "Structural Analysis of N-linked Sugar Chains of Human Blood Clotting Factor IX."J.Biochem.. 128(2). 175-180 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Makino, Y., Shimazaki, A., Omichi, K., and Hase, S.: "Processing pathway deduced from the structures of N-glycans in Carica papaya."J.Biochem.. 127(6). 1121-1126 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ashida, H., Yamamoto, K., Murata, T., Usui, T., and Kumagai, H.: "Characterization of Endo-α-N-acetylgalactosaminidase from Bacillus sp. and Syntheses of Neo-oligosaccharides Using Its Transglycosylation Activity."Arch.Biochem.Biophys.. 373. 394-400 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tani, M., Okino, Mitsutake, S., Tanigawa, T., Izu, H., and Ito, M.: "Purification and characterization of a novel ceramidase from mouse liver."J.Biol.Chem.. 275. 3462-3468 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tani, M., Okino, N., Mori, K., Tanigawa, T., Izu, H., and Ito, M.: "Molecular cloning of the full-length cDNA encoding mouse neutral ceramidase"J.Biol.Chem.. 275. 11229-11234 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Komori, H., Ichikawa, S., Hirabayashi, Y., and Ito, M.: "Regulation of UDP-glucose : ceramide glucosyltransferase-1 by ceramide."FEBS Letter. 475. 247-250 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Horibata, Y., Okino, N., Ichinose, S., Oomori, A., and Ito, M.: "Purification, characterization and cDNA cloning of a novel acidic endoglycoceramidase from the jellyfish, Cyanea nozakii."J.Biol.Chem.. 275. 31297-31304 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sakaguchi, K., Okino, N., Sueyoshi, N., Izu, H., and Ito, M.: "Cloning and expression of gene encoding a novel endoglycoceramidase of Rhodococcus sp.strain C9."J.Biochem.. 128. 145-152 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fujita, K., Tanaka, N., Sano, M., Kato, I., Asada, Y., and Takegawa, K.: "Synthesis of neoglycoenzymes with homogeneous N-linked oligosaccharides using immobilized endo-β-N-acetylglucosaminidase A."Biochem.Biophys.Res.Commun.. 67. 134-138 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fujita, K., Asada, Y., Yamamoto, K., and Takagaki, K.: "A plate assay for endo-β-N-acetylglucosaminidase activity using a chromogenic substrate synthesized by transglycosylation with Arthrobacter endo-β-N-acetylglucosaminidase."J.Biosci.Bioeng.. 90. 462-464 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takagaki, K., Munakata, H., Kakizaki, I., Majima, M., and Endo.M.: "Enzymatic reconstruction of dermatan sulfate."Biochem.Biophys.Res.Commun.. 270. 588-593 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takagaki, K., Munakata, H., Majima, M., Kakizaki, I., and Endo.M.: "Chimeric glycosaminoglycan oligosaccharides synthesized by enzymatic reconstruction and their use in substrate specificity determination of Streptococcus hyaluronidase"J.Biochem.. 127. 695-702 (2000)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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