2000 Fiscal Year Final Research Report Summary
Intrinsic optical imaging of neural activities using the laser speckle phenomenon
| Project/Area Number |
09358016
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| Research Category |
Grant-in-Aid for Scientific Research (A).
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Neuroscience in general
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
SATO Katsushige Tokyo Medical and Dental University Graduate School Assistant professor, 大学院・医歯学総合研究科, 助手 (80291342)
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| Co-Investigator(Kenkyū-buntansha) |
SAKAI Tetsuro Tokyo Medical and Dental University Graduate School Associate professor, 大学院・医歯学総合研究科, 助教授 (40153845)
MOMOSE-SATO Yoko Tokyo Medical and Dental University Graduate School Lecturer, 大学院・医歯学総合研究科, 講師 (70251501)
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| Project Period (FY) |
1997 – 2000
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| Keywords | laser speckle / cortical activity / noninvasive recording / brain mapping / optical recording / imaging |
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| Research Abstract |
We have constructed a new optical imaging system using the laser speckle phenomenon to detect neural activities from the central nervous system. This system makes us possible to detect not only intrinsic optical signals but also extrinsic optical signals with voltage-sensitive dyes. We have made a preliminary examination of feasibility of the new optical imaging system. Optical reflectance changes were detected in the rat somatosensory cortex in response to small whisker movements. This system was composed of a low noise CCD camera that provides a spatial resolution of 756×574 pixels, a 464ch photodiode array, an analog video enhancement amplifier and high resolution video displays, which were controlled by a high speed PC computer. A reference image was subtracted from the video signal. The resulting signal was then amplified and digitized by an image processor. This digitization of the differential-enhanced image was equivalent to more than 12 bit digitization. For the functional imaging, a single whisker was deflected for 1 or 2 sec while 8 frames of 500 msec each were recorded. For the first measurements, we examined the action spectrum of the intrinsic signal and compared the time course of the intrinsic signal at different wavelength. Moreover, we analyzed FFT spectrum of the evoked intrinsic signals. Consequently, we have been able to detect the relatively clear optical responses elicited during the whisker movement, and we could detect changes in FFT spectrum of the response.
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[Publications] Momose-Sato, Y., Komuro, H., Hirota, A., Sakai, T., Sato, K.and Kamino, K.: "Optical imaging of spatiotemporal patterning of neural responses in the embryonic chick superior cervical ganglion."Neuroscience. 90. 1069-1083 (1999)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Momose-Sato, Y., Sato, K., Arai, Y., Yazawa, I., Mochida, H.and Kamino, K.: "Evaluation of voltage-sensitive dyes for monitoring for long-term recording of neural activity in the hippocampus."Journal of Membrane Biology. 172. 145-157 (1999)
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[Publications] Sato, K., Yazawa, I., Mochida, H., Sasaki, S., Kamino, K.and Momose-Sato, Y.: "Optical detection of embryogenetic expression of vagal excitability in the rat brain stem."Neuroreport. 11. 3759-3763 (2000)
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「研究成果報告書概要(欧文)」より
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[Publications] Sato, K., Mochida, H., Sasaki, S., Yazawa, I., Kamino, K.and Momose-Sato, Y.: "Optical responses to micro-application of GABA agonists in the embryonic chick brain stem."Neuroreport. 12. 95-98 (2000)
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[Publications] Momose-Sato, Y., Sato, K., Mochida, H., Yazawa, I., Sasaki, S.and Kamino, K.: "Spreading depolarization waves triggered by vagal stimulation in the embryonic chick brain : optical evidence for intercellular communication in the developing CNS"Neuroscience. (in press). (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Mochida, H., Sato, K., Sasaki, S., Yazawa, I., Kamino, K.and Momose-Sate, Y.: "Effects of anisomycin on LTP in the hippocampal CA1 : Long-term analysis using optical recording."Neuroreport. (in press). (2001)
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[Publications] Mochida, H., Sato, K., Arai, Y., Sasaki, S., Itaru, Y., Kamino, K.and Momose-Sato, Y.: "Multiple-site optical recording reveals embryonic organization of synaptic networks in the chick spinal cord."European Journal of Neuroscience. (in press). (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Yazawa, I., Sasaki, S., Mochida, H., Kamino, K., Momose-Sato, Y.and Sato, K.: "Developmental changes in trial-to-trial variations in whisker barrel responses studied using intrinsic optical imaging : Comparison between normal and de-whiskered rats."Journal of Neurophysiology. (in press). (2001)
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「研究成果報告書概要(欧文)」より
